Control of Rhizoctonia solani with extracts from Ovidia andina = Control de Rhizoctonia solani con extractos de Ovidia andina
New alternatives are needed to control ubiquitous soilborne fungi like Rhizoctonia solani. This work evaluates the activity of extracts from Ovidia andina on this pathogen. Powdered dried leaves and stems were extracted by maceration with dichloromethane (CH2Cl2), methanol and ethanol. An aqueous ex...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Universidad Nacional de Cuyo
2018
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| Materias: | |
| Acceso en línea: | http://revista.fca.uncu.edu.ar/images/stories/pdfs/2018-02/Cp_25_Rivera_Marta-.pdf http://hdl.handle.net/20.500.12123/4086 |
| _version_ | 1855035257846235136 |
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| author | Mansilla, Javier Tito Tarcaya, Verónica P. Cufre, Ingrid M. Fabrizio, María del Carmen Wright, Eduardo Roberto Broussalis, Adriana M. Rivera, Marta Carolina |
| author_browse | Broussalis, Adriana M. Cufre, Ingrid M. Fabrizio, María del Carmen Mansilla, Javier Tito Rivera, Marta Carolina Tarcaya, Verónica P. Wright, Eduardo Roberto |
| author_facet | Mansilla, Javier Tito Tarcaya, Verónica P. Cufre, Ingrid M. Fabrizio, María del Carmen Wright, Eduardo Roberto Broussalis, Adriana M. Rivera, Marta Carolina |
| author_sort | Mansilla, Javier Tito |
| collection | INTA Digital |
| description | New alternatives are needed to control ubiquitous soilborne fungi like Rhizoctonia solani. This work evaluates the activity of extracts from Ovidia andina on this pathogen. Powdered dried leaves and stems were extracted by maceration with dichloromethane (CH2Cl2), methanol and ethanol. An aqueous extract was obtained by decoction, and the hydrodistillation residual water was the aqueous phase after essences production. A strain of R. solani was cultivated on potato dextrose agar supplemented with the extracts, at 1:100 and 1:1000 v/v. The CH2Cl2 extract at 1:100 reduced colony growth (p< 0.0001) and changed hyphal morphology. Five fractions were obtained from the CH2Cl2 extract in a glass column, and TLC analysis showed the presence of coumarins and flavonoids. The CH2Cl2 extract and fractions were diluted in CH2Cl2 or acetone. The efficiency to reduce pathogen growth of fractions 2f and 5f diluted in CH2Cl2 at 1:100 did not differ from the whole extract (p<0.0001). For an in vivo test, beetroot seed balls were incubated in soil infested with R. solani and treated by immersion in the CH2Cl2 extract at 1:100 for different periods of time. As a result, pathogen colonization was diminished by immersion for 120 s and seed germination was normal (p< 0.0001). Additionally, infested soil was treated with the CH2Cl2 extract, and inoculum concentration estimated using beetroot seed balls as baits diminished (p< 0.0001). These results lead us to conclude that O. andina is a source of antifungal components for crop protection against R. solani. |
| format | info:ar-repo/semantics/artículo |
| id | INTA4086 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Universidad Nacional de Cuyo |
| publisherStr | Universidad Nacional de Cuyo |
| record_format | dspace |
| spelling | INTA40862019-06-10T17:10:48Z Control of Rhizoctonia solani with extracts from Ovidia andina = Control de Rhizoctonia solani con extractos de Ovidia andina Mansilla, Javier Tito Tarcaya, Verónica P. Cufre, Ingrid M. Fabrizio, María del Carmen Wright, Eduardo Roberto Broussalis, Adriana M. Rivera, Marta Carolina Rhizoctonia solani Control de Enfermedades Hongos Entomopatogenos Extractos Vegetales Thymelaeaceae Disease Control Entomogenous Fungi Plant Extracts Ovidia andina New alternatives are needed to control ubiquitous soilborne fungi like Rhizoctonia solani. This work evaluates the activity of extracts from Ovidia andina on this pathogen. Powdered dried leaves and stems were extracted by maceration with dichloromethane (CH2Cl2), methanol and ethanol. An aqueous extract was obtained by decoction, and the hydrodistillation residual water was the aqueous phase after essences production. A strain of R. solani was cultivated on potato dextrose agar supplemented with the extracts, at 1:100 and 1:1000 v/v. The CH2Cl2 extract at 1:100 reduced colony growth (p< 0.0001) and changed hyphal morphology. Five fractions were obtained from the CH2Cl2 extract in a glass column, and TLC analysis showed the presence of coumarins and flavonoids. The CH2Cl2 extract and fractions were diluted in CH2Cl2 or acetone. The efficiency to reduce pathogen growth of fractions 2f and 5f diluted in CH2Cl2 at 1:100 did not differ from the whole extract (p<0.0001). For an in vivo test, beetroot seed balls were incubated in soil infested with R. solani and treated by immersion in the CH2Cl2 extract at 1:100 for different periods of time. As a result, pathogen colonization was diminished by immersion for 120 s and seed germination was normal (p< 0.0001). Additionally, infested soil was treated with the CH2Cl2 extract, and inoculum concentration estimated using beetroot seed balls as baits diminished (p< 0.0001). These results lead us to conclude that O. andina is a source of antifungal components for crop protection against R. solani. Resulta necesario generar alternativas para el control de hongos patógenos de suelo como Rhizoctonia solani. En este trabajo, se evaluó la actividad de extractos de Ovidia andina sobre una cepa del patógeno. Se realizaron extracciones a partir de hojas y tallos secos pulverizados, macerados con diclorometano (CH2Cl2), metanol y etanol. Se obtuvo un extracto acuoso por decocción y aguas residuales consistentes en la fase acuosa luego de la producción de esencias. Se cultivó una cepa de R. solani en agar papa glucosa suplementado con los extractos diluidos 1:100 o 1:1000 v/v. El extracto CH2Cl2 1:100 redujo el crecimiento de las colonias (p< 0,0001) y alteró la morfología de las hifas. Se obtuvieron 5 fracciones del extracto CH2Cl2 en una columna de vidrio y mediante TLC se detectó la presencia de cumarinas y flavonoides. Posteriormente, el extracto CH2Cl2 y sus fracciones se diluyeron en CH2Cl2 o acetona. La eficiencia de las fracciones 2f y 5f diluidas CH2Cl2 1:100 para reducir el crecimiento fúngico no difirió de la del extracto completo (p<0,0001). En un experimento in vivo, se incubaron glomérulos de remolacha en suelo infestado con R. solani y se sumergieron en el extracto CH2Cl2 1:100 durante tiempos variables. La colonización fúngica disminuyó mediante la inmersión durante 120 segundos, sin afectar la germinación (p< 0,0001). Adicionalmente, se trató suelo infestado con el extracto CH2Cl2 y disminuyó la concentración de inóculo del patógeno estimada utilizando glomérulos de remolacha como trampas (p< 0,0001). Estos resultados permiten concluir que O. andina es una fuente de componentes antifúngicos para la protección vegetal contra R. solani. Instituto de Floricultura Fil: Mansilla, Javier Tito. Universidad Nacional de San Martín. Posgrado; Argentina Fil: Tarcaya, Verónica P. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Cufre, Ingrid M. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Fabrizio, María del Carmen. Universidad de Buenos Aires. Facultad de Agronomía; Argentina Fil: Wright, Eduardo Roberto. Universidad de Buenos Aires. Facultad de Agronomía; Argentina Fil: Broussalis, Adriana M. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Rivera, Marta Carolina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina 2018-12-14T13:08:40Z 2018-12-14T13:08:40Z 2018 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://revista.fca.uncu.edu.ar/images/stories/pdfs/2018-02/Cp_25_Rivera_Marta-.pdf http://hdl.handle.net/20.500.12123/4086 0370-4661 1853-8665 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Universidad Nacional de Cuyo Revista de la Facultad de Ciencias Agrarias / Universidad Nacional de Cuyo 50 (2) : 355-368 (2018) |
| spellingShingle | Rhizoctonia solani Control de Enfermedades Hongos Entomopatogenos Extractos Vegetales Thymelaeaceae Disease Control Entomogenous Fungi Plant Extracts Ovidia andina Mansilla, Javier Tito Tarcaya, Verónica P. Cufre, Ingrid M. Fabrizio, María del Carmen Wright, Eduardo Roberto Broussalis, Adriana M. Rivera, Marta Carolina Control of Rhizoctonia solani with extracts from Ovidia andina = Control de Rhizoctonia solani con extractos de Ovidia andina |
| title | Control of Rhizoctonia solani with extracts from Ovidia andina = Control de Rhizoctonia solani con extractos de Ovidia andina |
| title_full | Control of Rhizoctonia solani with extracts from Ovidia andina = Control de Rhizoctonia solani con extractos de Ovidia andina |
| title_fullStr | Control of Rhizoctonia solani with extracts from Ovidia andina = Control de Rhizoctonia solani con extractos de Ovidia andina |
| title_full_unstemmed | Control of Rhizoctonia solani with extracts from Ovidia andina = Control de Rhizoctonia solani con extractos de Ovidia andina |
| title_short | Control of Rhizoctonia solani with extracts from Ovidia andina = Control de Rhizoctonia solani con extractos de Ovidia andina |
| title_sort | control of rhizoctonia solani with extracts from ovidia andina control de rhizoctonia solani con extractos de ovidia andina |
| topic | Rhizoctonia solani Control de Enfermedades Hongos Entomopatogenos Extractos Vegetales Thymelaeaceae Disease Control Entomogenous Fungi Plant Extracts Ovidia andina |
| url | http://revista.fca.uncu.edu.ar/images/stories/pdfs/2018-02/Cp_25_Rivera_Marta-.pdf http://hdl.handle.net/20.500.12123/4086 |
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