Assessment of cold tolerance at early developmental stages and allelic variation at candidate genes in South American rice germplasm
Rice is susceptible to cold during early developmental stages. Most tolerant cultivars have been developed for other conditions than those occurring in South America’s rice growing areas, or their grain type is not suitable for the local markets. If locally adapted varieties were available, growers...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2018
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| Acceso en línea: | https://link.springer.com/article/10.1007/s10681-014-1078-4 http://hdl.handle.net/20.500.12123/4026 https://doi.org/10.1007/s10681-014-1078-4 |
| _version_ | 1855483350816391168 |
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| author | Pachecoy, Maria Ines Ramirez, Ignacio Abel Marín, Alfredo Pontaroli, Ana Clara |
| author_browse | Marín, Alfredo Pachecoy, Maria Ines Pontaroli, Ana Clara Ramirez, Ignacio Abel |
| author_facet | Pachecoy, Maria Ines Ramirez, Ignacio Abel Marín, Alfredo Pontaroli, Ana Clara |
| author_sort | Pachecoy, Maria Ines |
| collection | INTA Digital |
| description | Rice is susceptible to cold during early developmental stages. Most tolerant cultivars have been developed for other conditions than those occurring in South America’s rice growing areas, or their grain type is not suitable for the local markets. If locally adapted varieties were available, growers could anticipate sowing date, making flowering time coincide with the moment of maximum solar radiation and increasing yields. In this work, 116 rice inbred lines and varieties of diverse origin within the South American gene pool were tested for seedling survival and germination percentage under low temperature in controlled conditions. As a result, lines used as controls responded as expected, whereas lines with similar behavior to controls, intermediate between susceptible and tolerant controls and more extreme than controls were detected at both seedling and germination stages. Allelic variation at candidate genes OsGSTZ1, OsGSTZ2 and OsCDPK13 was analyzed in a subset of ten contrasting lines. Ten out of thirty-four polymorphisms detected in all three genes were associated with cold tolerance in these lines. A functional marker, designed from an aminoacid-changing SNP found in gene OsGSTZ2, was tested in the 116 lines. As a result, a tight association was observed between cold tolerance and marker score. In conclusion, wide variability for cold tolerance at early developmental stages has been detected in rice advanced breeding materials that are adapted to local growing conditions. Also, a functional marker tightly associated with the trait is available for performing marker assisted selection |
| format | Artículo |
| id | INTA4026 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA40262018-12-07T14:20:46Z Assessment of cold tolerance at early developmental stages and allelic variation at candidate genes in South American rice germplasm Pachecoy, Maria Ines Ramirez, Ignacio Abel Marín, Alfredo Pontaroli, Ana Clara Arroz Tolerancia al Frío Variación Genética Germoplasma Marcadores Genéticos Rice Cold Tolerance Genetic Variation Germplasm Genetic Markers Resistencia al Frío América del Sur Rice is susceptible to cold during early developmental stages. Most tolerant cultivars have been developed for other conditions than those occurring in South America’s rice growing areas, or their grain type is not suitable for the local markets. If locally adapted varieties were available, growers could anticipate sowing date, making flowering time coincide with the moment of maximum solar radiation and increasing yields. In this work, 116 rice inbred lines and varieties of diverse origin within the South American gene pool were tested for seedling survival and germination percentage under low temperature in controlled conditions. As a result, lines used as controls responded as expected, whereas lines with similar behavior to controls, intermediate between susceptible and tolerant controls and more extreme than controls were detected at both seedling and germination stages. Allelic variation at candidate genes OsGSTZ1, OsGSTZ2 and OsCDPK13 was analyzed in a subset of ten contrasting lines. Ten out of thirty-four polymorphisms detected in all three genes were associated with cold tolerance in these lines. A functional marker, designed from an aminoacid-changing SNP found in gene OsGSTZ2, was tested in the 116 lines. As a result, a tight association was observed between cold tolerance and marker score. In conclusion, wide variability for cold tolerance at early developmental stages has been detected in rice advanced breeding materials that are adapted to local growing conditions. Also, a functional marker tightly associated with the trait is available for performing marker assisted selection EEA Balcarce Fil: Pachecoy, Maria Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Corrientes; Argentina Fil: Ramirez, Ignacio Abel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Monsanto’s Beachell-Borlaug International Fellowship Program; Argentina Fil: Marín, Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Corrientes; Argentina Fil: Pontaroli, Ana Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-12-07T14:19:20Z 2018-12-07T14:19:20Z 2014-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s10681-014-1078-4 http://hdl.handle.net/20.500.12123/4026 0014-2336 1573-5060 https://doi.org/10.1007/s10681-014-1078-4 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Euphytica 197 (3) : 423–434 (June 2014) |
| spellingShingle | Arroz Tolerancia al Frío Variación Genética Germoplasma Marcadores Genéticos Rice Cold Tolerance Genetic Variation Germplasm Genetic Markers Resistencia al Frío América del Sur Pachecoy, Maria Ines Ramirez, Ignacio Abel Marín, Alfredo Pontaroli, Ana Clara Assessment of cold tolerance at early developmental stages and allelic variation at candidate genes in South American rice germplasm |
| title | Assessment of cold tolerance at early developmental stages and allelic variation at candidate genes in South American rice germplasm |
| title_full | Assessment of cold tolerance at early developmental stages and allelic variation at candidate genes in South American rice germplasm |
| title_fullStr | Assessment of cold tolerance at early developmental stages and allelic variation at candidate genes in South American rice germplasm |
| title_full_unstemmed | Assessment of cold tolerance at early developmental stages and allelic variation at candidate genes in South American rice germplasm |
| title_short | Assessment of cold tolerance at early developmental stages and allelic variation at candidate genes in South American rice germplasm |
| title_sort | assessment of cold tolerance at early developmental stages and allelic variation at candidate genes in south american rice germplasm |
| topic | Arroz Tolerancia al Frío Variación Genética Germoplasma Marcadores Genéticos Rice Cold Tolerance Genetic Variation Germplasm Genetic Markers Resistencia al Frío América del Sur |
| url | https://link.springer.com/article/10.1007/s10681-014-1078-4 http://hdl.handle.net/20.500.12123/4026 https://doi.org/10.1007/s10681-014-1078-4 |
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