Optimization of production of chlamydospores of the nematode-trapping fungus Duddingtonia flagrans in solid culture media
The large-scale production of nematophagous fungi as agents of biological control is one of the main challenges to be commercially used. In order to improve growth of microorganism in a culture medium, the addition of growth inducer is common. At the moment, the action of their addition in the mycel...
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Springer
2018
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| Online Access: | https://link.springer.com/article/10.1007/s00436-012-3231-0 http://hdl.handle.net/20.500.12123/4004 https://doi.org/10.1007/s00436-012-3231-0 |
| _version_ | 1855483346674515968 |
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| author | Sagüés, María Federica Fusé, Luis Alberto Iglesias, Lucía Emilia Moreno, Fabiana Carina Saumell, Carlos Alfredo |
| author_browse | Fusé, Luis Alberto Iglesias, Lucía Emilia Moreno, Fabiana Carina Sagüés, María Federica Saumell, Carlos Alfredo |
| author_facet | Sagüés, María Federica Fusé, Luis Alberto Iglesias, Lucía Emilia Moreno, Fabiana Carina Saumell, Carlos Alfredo |
| author_sort | Sagüés, María Federica |
| collection | INTA Digital |
| description | The large-scale production of nematophagous fungi as agents of biological control is one of the main challenges to be commercially used. In order to improve growth of microorganism in a culture medium, the addition of growth inducer is common. At the moment, the action of their addition in the mycelia growth and sporulation rate of nematophagous fungi is not known. The purpose of this trial was to evaluate the sporulation rate of Duddingtonia flagrans by adding two growth inducers, meso-inositol and Tween 80, both at 0.5 % in a traditional culture medium Sabouraud glucose agar (SGA) and also in a traditional culture medium enriched with wheat flour and milk powder. From a traditional sterile culture of D. flagrans, four groups were made: SGA; Sabouraud glucose agar–meso-inositol 0.5 %; Sabouraud glucose agar–Tween 80 0.5 %; and Sabouraud glucose agar-enriched (SGA-E). These media were placed at a constant temperature of 27 °C for 4 weeks. Following this, chlamydospores were gently rinsed off with sterile water and counted using a Neubauer haematocytometer to estimate the number of chlamydospores per millilitre of water. The addition of meso-inositol 0.5 % to SGA promoted a significant increase (p < 0.05) in chlamydospore production obtaining an average of 51,715,000 chlamydospores per Petri dish. The highest chlamydospore concentration was observed in the SGA-E in comparison with SGA (p < 0.01) obtaining an average of 208,760,000 chlamydospores. The aim of this study was to obtain basic knowledge regarding the effect of enriched culture medium and growth-inducing meso-inositol and Tween 80 on mycelial growth and production of chlamydospores. |
| format | Artículo |
| id | INTA4004 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA40042018-12-04T12:07:20Z Optimization of production of chlamydospores of the nematode-trapping fungus Duddingtonia flagrans in solid culture media Sagüés, María Federica Fusé, Luis Alberto Iglesias, Lucía Emilia Moreno, Fabiana Carina Saumell, Carlos Alfredo Clamidosporas Hongos Nematófagos Control Biológico Experimentación en Laboratorio Nematoda Medio de Cultivo Chlamydospores Nematophagous Fungi Biological Control Laboratory Experimentation Culture Media Duddingtonia flagrans Nematodes Gastrointestinales The large-scale production of nematophagous fungi as agents of biological control is one of the main challenges to be commercially used. In order to improve growth of microorganism in a culture medium, the addition of growth inducer is common. At the moment, the action of their addition in the mycelia growth and sporulation rate of nematophagous fungi is not known. The purpose of this trial was to evaluate the sporulation rate of Duddingtonia flagrans by adding two growth inducers, meso-inositol and Tween 80, both at 0.5 % in a traditional culture medium Sabouraud glucose agar (SGA) and also in a traditional culture medium enriched with wheat flour and milk powder. From a traditional sterile culture of D. flagrans, four groups were made: SGA; Sabouraud glucose agar–meso-inositol 0.5 %; Sabouraud glucose agar–Tween 80 0.5 %; and Sabouraud glucose agar-enriched (SGA-E). These media were placed at a constant temperature of 27 °C for 4 weeks. Following this, chlamydospores were gently rinsed off with sterile water and counted using a Neubauer haematocytometer to estimate the number of chlamydospores per millilitre of water. The addition of meso-inositol 0.5 % to SGA promoted a significant increase (p < 0.05) in chlamydospore production obtaining an average of 51,715,000 chlamydospores per Petri dish. The highest chlamydospore concentration was observed in the SGA-E in comparison with SGA (p < 0.01) obtaining an average of 208,760,000 chlamydospores. The aim of this study was to obtain basic knowledge regarding the effect of enriched culture medium and growth-inducing meso-inositol and Tween 80 on mycelial growth and production of chlamydospores. EEA Balcarce Fil: Sagüés, María Federica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina Fil: Fusé, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Iglesias, Lucía Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Moreno, Fabiana Carina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Saumell, Carlos Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina 2018-12-04T12:04:42Z 2018-12-04T12:04:42Z 2013-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s00436-012-3231-0 http://hdl.handle.net/20.500.12123/4004 0932-0113 1432-1955 https://doi.org/10.1007/s00436-012-3231-0 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Parasitology Research 112 (3) : 1047–1051 (March 2013) |
| spellingShingle | Clamidosporas Hongos Nematófagos Control Biológico Experimentación en Laboratorio Nematoda Medio de Cultivo Chlamydospores Nematophagous Fungi Biological Control Laboratory Experimentation Culture Media Duddingtonia flagrans Nematodes Gastrointestinales Sagüés, María Federica Fusé, Luis Alberto Iglesias, Lucía Emilia Moreno, Fabiana Carina Saumell, Carlos Alfredo Optimization of production of chlamydospores of the nematode-trapping fungus Duddingtonia flagrans in solid culture media |
| title | Optimization of production of chlamydospores of the nematode-trapping fungus Duddingtonia flagrans in solid culture media |
| title_full | Optimization of production of chlamydospores of the nematode-trapping fungus Duddingtonia flagrans in solid culture media |
| title_fullStr | Optimization of production of chlamydospores of the nematode-trapping fungus Duddingtonia flagrans in solid culture media |
| title_full_unstemmed | Optimization of production of chlamydospores of the nematode-trapping fungus Duddingtonia flagrans in solid culture media |
| title_short | Optimization of production of chlamydospores of the nematode-trapping fungus Duddingtonia flagrans in solid culture media |
| title_sort | optimization of production of chlamydospores of the nematode trapping fungus duddingtonia flagrans in solid culture media |
| topic | Clamidosporas Hongos Nematófagos Control Biológico Experimentación en Laboratorio Nematoda Medio de Cultivo Chlamydospores Nematophagous Fungi Biological Control Laboratory Experimentation Culture Media Duddingtonia flagrans Nematodes Gastrointestinales |
| url | https://link.springer.com/article/10.1007/s00436-012-3231-0 http://hdl.handle.net/20.500.12123/4004 https://doi.org/10.1007/s00436-012-3231-0 |
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