Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation
This study examined the immunological responses of pregnant cattle and their foetuses following an experimental challenge with live Neospora caninum tachyzoites at day 210 of gestation. Animals were bled prior to and weekly throughout the experiment and sacrificed at 14, 28, 42 and 56 days post inoc...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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BMC
2018
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| Materias: | |
| Acceso en línea: | https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-44-91 http://hdl.handle.net/20.500.12123/4000 https://doi.org/10.1186/1297-9716-44-91 |
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| author | Bartley, Paul M. Katzer, Frank Rocchi, Mara Maley, Stephen W. Benavides-Silván, Julio Nath, Mintu Pang, Yvonne Canton, German Jose Thomson, Jackie Chianini, Francesca Innes, Elisabeth A. |
| author_browse | Bartley, Paul M. Benavides-Silván, Julio Canton, German Jose Chianini, Francesca Innes, Elisabeth A. Katzer, Frank Maley, Stephen W. Nath, Mintu Pang, Yvonne Rocchi, Mara Thomson, Jackie |
| author_facet | Bartley, Paul M. Katzer, Frank Rocchi, Mara Maley, Stephen W. Benavides-Silván, Julio Nath, Mintu Pang, Yvonne Canton, German Jose Thomson, Jackie Chianini, Francesca Innes, Elisabeth A. |
| author_sort | Bartley, Paul M. |
| collection | INTA Digital |
| description | This study examined the immunological responses of pregnant cattle and their foetuses following an experimental challenge with live Neospora caninum tachyzoites at day 210 of gestation. Animals were bled prior to and weekly throughout the experiment and sacrificed at 14, 28, 42 and 56 days post inoculation (dpi). At post mortem examination, samples of lymph nodes and spleen were collected from both dam and foetus for immunological analysis. Subcutaneous (sc) inoculation over the left prefemoral (LPF) lymph node of pregnant cattle at day 210 of gestation, led to the vertical transmission of parasites by 14 dpi, however no foetal deaths were observed in the infected animals. Foetuses from infected dams mounted Neospora-specific humoral and cell-mediated immune (CMI) responses by 14 dpi. These responses involved anti-Neospora IgG, antigen-specific lymphocyte proliferation, and the production of the cytokines IFN–γ, interleukin (IL)-4 and IL-10. There was also evidence of innate immunity during the response against Neospora from infected dams, with statistically significant (p < 0.05) increases in mean expression of toll like receptors (TLR)-2 on 56 dpi in maternal spleen, LPF, right prefemoral (RPF), left uterine (LUL) and right uterine (RUL) lymph nodes and TLR-9 in retropharyngeal (RLN), LPF and RPF lymph nodes from 28 dpi. Statistically significant (p < 0.05) increases in mean TLR-9 were detected in spleen samples from foetuses of infected dams, compared to the foetuses from control animals. Our results show that vertical transmission of the parasite occurred in all infected dams, with their foetuses showing effective Neospora-specific cell mediated, humoral and innate immune responses. |
| format | Artículo |
| id | INTA4000 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | BMC |
| publisherStr | BMC |
| record_format | dspace |
| spelling | INTA40002018-12-03T17:22:30Z Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation Bartley, Paul M. Katzer, Frank Rocchi, Mara Maley, Stephen W. Benavides-Silván, Julio Nath, Mintu Pang, Yvonne Canton, German Jose Thomson, Jackie Chianini, Francesca Innes, Elisabeth A. Ganado Bovino Enfermedades de los Animales Neospora caninum Respuesta Inmunológica Feto Cattle Animal Diseases Immune Response Foetus This study examined the immunological responses of pregnant cattle and their foetuses following an experimental challenge with live Neospora caninum tachyzoites at day 210 of gestation. Animals were bled prior to and weekly throughout the experiment and sacrificed at 14, 28, 42 and 56 days post inoculation (dpi). At post mortem examination, samples of lymph nodes and spleen were collected from both dam and foetus for immunological analysis. Subcutaneous (sc) inoculation over the left prefemoral (LPF) lymph node of pregnant cattle at day 210 of gestation, led to the vertical transmission of parasites by 14 dpi, however no foetal deaths were observed in the infected animals. Foetuses from infected dams mounted Neospora-specific humoral and cell-mediated immune (CMI) responses by 14 dpi. These responses involved anti-Neospora IgG, antigen-specific lymphocyte proliferation, and the production of the cytokines IFN–γ, interleukin (IL)-4 and IL-10. There was also evidence of innate immunity during the response against Neospora from infected dams, with statistically significant (p < 0.05) increases in mean expression of toll like receptors (TLR)-2 on 56 dpi in maternal spleen, LPF, right prefemoral (RPF), left uterine (LUL) and right uterine (RUL) lymph nodes and TLR-9 in retropharyngeal (RLN), LPF and RPF lymph nodes from 28 dpi. Statistically significant (p < 0.05) increases in mean TLR-9 were detected in spleen samples from foetuses of infected dams, compared to the foetuses from control animals. Our results show that vertical transmission of the parasite occurred in all infected dams, with their foetuses showing effective Neospora-specific cell mediated, humoral and innate immune responses. EEA Balcarce Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido Fil: Katzer, Frank. Moredun Research Institute; Reino Unido Fil: Rocchi, Mara. Moredun Research Institute; Reino Unido Fil: Maley, Stephen W. Moredun Research Institute; Reino Unido Fil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; España Fil: Nath, Mintu. Biomathematics & Statistics Scotland; Reino Unidos Fil: Pang, Yvonne. Moredun Research Institute; Reino Unido Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Thomson, Jackie. Moredun Research Institute; Reino Unido Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido 2018-12-03T17:21:18Z 2018-12-03T17:21:18Z 2013-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-44-91 http://hdl.handle.net/20.500.12123/4000 0928-4249 1297-9716 https://doi.org/10.1186/1297-9716-44-91 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BMC Veterinary Research 44 : 91 (2013) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Neospora caninum Respuesta Inmunológica Feto Cattle Animal Diseases Immune Response Foetus Bartley, Paul M. Katzer, Frank Rocchi, Mara Maley, Stephen W. Benavides-Silván, Julio Nath, Mintu Pang, Yvonne Canton, German Jose Thomson, Jackie Chianini, Francesca Innes, Elisabeth A. Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation |
| title | Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation |
| title_full | Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation |
| title_fullStr | Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation |
| title_full_unstemmed | Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation |
| title_short | Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation |
| title_sort | development of maternal and foetal immune responses in cattle following experimental challenge with neospora caninum at day 210 of gestation |
| topic | Ganado Bovino Enfermedades de los Animales Neospora caninum Respuesta Inmunológica Feto Cattle Animal Diseases Immune Response Foetus |
| url | https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-44-91 http://hdl.handle.net/20.500.12123/4000 https://doi.org/10.1186/1297-9716-44-91 |
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