Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil
The objectives of the present study were to assess the population structure and genetic diversity in traditional sweet cassava cultivars collected in “backyard” cultivations in the municipalities of Maringá, Cianorte, and Toledo, State of Paraná, Southern Brazil, using 13 SSR molecular markers. All...
| Main Authors: | , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Springer
2018
|
| Subjects: | |
| Online Access: | https://link.springer.com/article/10.1007/s11105-016-0995-x http://hdl.handle.net/20.500.12123/3996 https://doi.org/10.1007/s11105-016-0995-x |
| _version_ | 1855035241687678976 |
|---|---|
| author | Ortiz, Alex Henrique Tiene Rocha, Vanesca Priscila Camargo Moiana, Leonel Domingos Vidigal, Maria Celeste Gonçalves Galvan, Marta Zulema Vidigal Filho, Pedro Soares |
| author_browse | Galvan, Marta Zulema Moiana, Leonel Domingos Ortiz, Alex Henrique Tiene Rocha, Vanesca Priscila Camargo Vidigal Filho, Pedro Soares Vidigal, Maria Celeste Gonçalves |
| author_facet | Ortiz, Alex Henrique Tiene Rocha, Vanesca Priscila Camargo Moiana, Leonel Domingos Vidigal, Maria Celeste Gonçalves Galvan, Marta Zulema Vidigal Filho, Pedro Soares |
| author_sort | Ortiz, Alex Henrique Tiene |
| collection | INTA Digital |
| description | The objectives of the present study were to assess the population structure and genetic diversity in traditional sweet cassava cultivars collected in “backyard” cultivations in the municipalities of Maringá, Cianorte, and Toledo, State of Paraná, Southern Brazil, using 13 SSR molecular markers. All the loci analyzed were considered polymorphic with a mean of 3.15 alleles per locus; the mean polymorphism information content (PIC) value found was 0.4598, indicating that the primers were reasonably informative and the heterozygosity amplitude observed ranged from 0.0270 (GA134) to 0.8718 (SSRY45), with a mean of 0.4762, while the mean genetic diversity obtained was 0.5407, ranging from 0.3138 (GA21) to 0.6502 (GA140). The most divergent combinations were BGM434T-BGM20M, BGM35C-BGM20M, BGM430T-BGM232M, BGM430T-BGM164M, BGM430T-BGM322M, and BGM35C-BGM164M. The analysis of population structure distributed the traditional cultivars assessed in four different groups. The analysis of molecular variance (AMOVA) estimated that 11, 15, and 74 % of the variance were between groups, between individuals within groups, and between individuals within the population as a whole, respectively. The F is (0.170) and F it (0.259) values indicated a number of heterozygotes present in the population under study lower than that necessary to reach the Hardy-Weinberg equilibrium. The genetic variability found among the traditional sweet cassava cultivars assessed was considered wide, and the groups that were most distant were mostly cultivars from Toledo and Maringá. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3996 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA39962018-12-03T12:29:48Z Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil Ortiz, Alex Henrique Tiene Rocha, Vanesca Priscila Camargo Moiana, Leonel Domingos Vidigal, Maria Celeste Gonçalves Galvan, Marta Zulema Vidigal Filho, Pedro Soares Mandioca Variedades Genética Variación Genética Marcadores Genéticos Polimorfismo Cassava Varieties Genetics Genetic Variation Genetic Markers Polymorphism Marcadores Moleculares Mandioca Dulce Brasil The objectives of the present study were to assess the population structure and genetic diversity in traditional sweet cassava cultivars collected in “backyard” cultivations in the municipalities of Maringá, Cianorte, and Toledo, State of Paraná, Southern Brazil, using 13 SSR molecular markers. All the loci analyzed were considered polymorphic with a mean of 3.15 alleles per locus; the mean polymorphism information content (PIC) value found was 0.4598, indicating that the primers were reasonably informative and the heterozygosity amplitude observed ranged from 0.0270 (GA134) to 0.8718 (SSRY45), with a mean of 0.4762, while the mean genetic diversity obtained was 0.5407, ranging from 0.3138 (GA21) to 0.6502 (GA140). The most divergent combinations were BGM434T-BGM20M, BGM35C-BGM20M, BGM430T-BGM232M, BGM430T-BGM164M, BGM430T-BGM322M, and BGM35C-BGM164M. The analysis of population structure distributed the traditional cultivars assessed in four different groups. The analysis of molecular variance (AMOVA) estimated that 11, 15, and 74 % of the variance were between groups, between individuals within groups, and between individuals within the population as a whole, respectively. The F is (0.170) and F it (0.259) values indicated a number of heterozygotes present in the population under study lower than that necessary to reach the Hardy-Weinberg equilibrium. The genetic variability found among the traditional sweet cassava cultivars assessed was considered wide, and the groups that were most distant were mostly cultivars from Toledo and Maringá. EEA Salta Fil: Ortiz, Alex Henrique Tiene. Universidade Estadual de Maringá. Pós-Graduação em Genética e Melhoramento; Brasil Fil: Rocha, Vanesca Priscila Camargo. Universidade Estadual de Maringá. Pós-Graduação em Genética e Melhoramento; Brasil Fil: Moiana, Leonel Domingos. Universidade Estadual de Maringá. Pós-Graduação em Genética e Melhoramento; Brasil. Instituto de Investigação Agrária; Mozambique Fil: Vidigal, Maria Celeste Gonçalves. Universidade Estadual de Maringá. Pós-Graduação em Genética e Melhoramento; Brasil Fil: Galvan, Marta Zulema. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Fil: Vidigal Filho, Pedro Soares. Universidade Estadual de Maringá. Pós-Graduação em Genética e Melhoramento; Brasil 2018-12-03T12:28:20Z 2018-12-03T12:28:20Z 2016-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s11105-016-0995-x http://hdl.handle.net/20.500.12123/3996 0735-9640 1572-9818 https://doi.org/10.1007/s11105-016-0995-x eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Plant Molecular Biology Reporter 34 (6) : 1153–1166 (December 2016) |
| spellingShingle | Mandioca Variedades Genética Variación Genética Marcadores Genéticos Polimorfismo Cassava Varieties Genetics Genetic Variation Genetic Markers Polymorphism Marcadores Moleculares Mandioca Dulce Brasil Ortiz, Alex Henrique Tiene Rocha, Vanesca Priscila Camargo Moiana, Leonel Domingos Vidigal, Maria Celeste Gonçalves Galvan, Marta Zulema Vidigal Filho, Pedro Soares Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil |
| title | Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil |
| title_full | Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil |
| title_fullStr | Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil |
| title_full_unstemmed | Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil |
| title_short | Population Structure and Genetic Diversity in Sweet Cassava Cultivars from Paraná, Brazil |
| title_sort | population structure and genetic diversity in sweet cassava cultivars from parana brazil |
| topic | Mandioca Variedades Genética Variación Genética Marcadores Genéticos Polimorfismo Cassava Varieties Genetics Genetic Variation Genetic Markers Polymorphism Marcadores Moleculares Mandioca Dulce Brasil |
| url | https://link.springer.com/article/10.1007/s11105-016-0995-x http://hdl.handle.net/20.500.12123/3996 https://doi.org/10.1007/s11105-016-0995-x |
| work_keys_str_mv | AT ortizalexhenriquetiene populationstructureandgeneticdiversityinsweetcassavacultivarsfromparanabrazil AT rochavanescapriscilacamargo populationstructureandgeneticdiversityinsweetcassavacultivarsfromparanabrazil AT moianaleoneldomingos populationstructureandgeneticdiversityinsweetcassavacultivarsfromparanabrazil AT vidigalmariacelestegoncalves populationstructureandgeneticdiversityinsweetcassavacultivarsfromparanabrazil AT galvanmartazulema populationstructureandgeneticdiversityinsweetcassavacultivarsfromparanabrazil AT vidigalfilhopedrosoares populationstructureandgeneticdiversityinsweetcassavacultivarsfromparanabrazil |