A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus
The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mu...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Nature Publishing Group
2018
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| Acceso en línea: | https://www.nature.com/articles/s41598-017-17309-x http://hdl.handle.net/20.500.12123/3988 https://doi.org/10.1038/s41598-017-17309-x |
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| author | Garcia Guizzo, Melina Parizi, Luís Fernando Nunes, Rodrigo Dutra Schama, Renata Albano, Rodolpho M. Tirloni, Lucas Oldiges, Daiane Patrícia Pilz Vieira, Ricardo Cruz Oliveira, Wanderson Henrique De Souza Leite, Milane Gonzalez, Sergio Alberto Farber, Marisa Diana Martins, Orlando Da Silva Vaz, Itabajara Oliveira, Pedro L. |
| author_browse | Albano, Rodolpho M. Cruz Oliveira, Wanderson Henrique Da Silva Vaz, Itabajara De Souza Leite, Milane Farber, Marisa Diana Garcia Guizzo, Melina Gonzalez, Sergio Alberto Martins, Orlando Nunes, Rodrigo Dutra Oldiges, Daiane Patrícia Oliveira, Pedro L. Parizi, Luís Fernando Pilz Vieira, Ricardo Schama, Renata Tirloni, Lucas |
| author_facet | Garcia Guizzo, Melina Parizi, Luís Fernando Nunes, Rodrigo Dutra Schama, Renata Albano, Rodolpho M. Tirloni, Lucas Oldiges, Daiane Patrícia Pilz Vieira, Ricardo Cruz Oliveira, Wanderson Henrique De Souza Leite, Milane Gonzalez, Sergio Alberto Farber, Marisa Diana Martins, Orlando Da Silva Vaz, Itabajara Oliveira, Pedro L. |
| author_sort | Garcia Guizzo, Melina |
| collection | INTA Digital |
| description | The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mutualist symbiont, comprising 98% of the 16S rRNA sequences in both eggs and larvae. Sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic cofactors that may provide a nutritional complement to the tick host. The CERM was abundant in ovary and Malpighian tubule of fully engorged female. Tetracycline treatment of either the tick or the vertebrate host reduced levels of bacteria in progeny in 74% for eggs and 90% for larvae without major impact neither on the reproductive fitness of the adult female or on embryo development. However, CERM proved to be essential for the tick to reach the adult life stage, as under antibiotic treatment no tick was able to progress beyond the metanymph stage. Data presented here suggest that interference in the symbiotic CERM-R. microplus relationship may be useful to the development of alternative control methods, highlighting the interdependence between ticks and their endosymbionts. |
| format | Artículo |
| id | INTA3988 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Nature Publishing Group |
| publisherStr | Nature Publishing Group |
| record_format | dspace |
| spelling | INTA39882018-11-29T11:24:53Z A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus Garcia Guizzo, Melina Parizi, Luís Fernando Nunes, Rodrigo Dutra Schama, Renata Albano, Rodolpho M. Tirloni, Lucas Oldiges, Daiane Patrícia Pilz Vieira, Ricardo Cruz Oliveira, Wanderson Henrique De Souza Leite, Milane Gonzalez, Sergio Alberto Farber, Marisa Diana Martins, Orlando Da Silva Vaz, Itabajara Oliveira, Pedro L. Coxiella Rhipicephalus Metastigmata Simbionte Garrapatas Symbiont Ticks Rhipicephalus microplus The cattle tick Rhipicephalus microplus is a hematophagous ectoparasite that causes important economic losses in livestock. Different species of ticks harbor a symbiont bacterium of the genus Coxiella. It was showed that a Coxiella endosymbiont from R. microplus (CERM) is a vertically transmitted mutualist symbiont, comprising 98% of the 16S rRNA sequences in both eggs and larvae. Sequencing of the bacterial genome revealed genes for biosynthetic pathways for several vitamins and key metabolic cofactors that may provide a nutritional complement to the tick host. The CERM was abundant in ovary and Malpighian tubule of fully engorged female. Tetracycline treatment of either the tick or the vertebrate host reduced levels of bacteria in progeny in 74% for eggs and 90% for larvae without major impact neither on the reproductive fitness of the adult female or on embryo development. However, CERM proved to be essential for the tick to reach the adult life stage, as under antibiotic treatment no tick was able to progress beyond the metanymph stage. Data presented here suggest that interference in the symbiotic CERM-R. microplus relationship may be useful to the development of alternative control methods, highlighting the interdependence between ticks and their endosymbionts. Instituto de Biotecnología Fil: Garcia Guizzo, Melina. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil Fil: Parizi, Luís Fernando. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil Fil: Nunes, Rodrigo Dutra. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil Fil: Schama, Renata. Laboratório de Biologia Computacional e Sistemas; Brasil Fil: Albano, Rodolpho M. Universidade do Estado do Rio de Janeiro. Departamento de Bioquímica; Brasil Fil: Tirloni, Lucas. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil Fil: Oldiges, Daiane Patrícia. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil Fil: Pilz Vieira, Ricardo. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil Fil: Cruz Oliveira, Wanderson Henrique. Universidade Federal Rural do Rio de Janeiro. Departamento de Química; Brasil Fil: De Souza Leite, Milane. Universidade Federal Rural do Rio de Janeiro. Departamento de Química; Brasil Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Martins, Orlando. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil Fil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil. Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária; Brasil. Universidade Federal Rural do Rio de Janeiro. Departamento de Química; Brasil Fil: Oliveira, Pedro L.. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil 2018-11-29T11:13:12Z 2018-11-29T11:13:12Z 2017-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.nature.com/articles/s41598-017-17309-x http://hdl.handle.net/20.500.12123/3988 2045-2322 https://doi.org/10.1038/s41598-017-17309-x eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Nature Publishing Group Scientific Reports 7: 17554 (Diciembre 2017) |
| spellingShingle | Coxiella Rhipicephalus Metastigmata Simbionte Garrapatas Symbiont Ticks Rhipicephalus microplus Garcia Guizzo, Melina Parizi, Luís Fernando Nunes, Rodrigo Dutra Schama, Renata Albano, Rodolpho M. Tirloni, Lucas Oldiges, Daiane Patrícia Pilz Vieira, Ricardo Cruz Oliveira, Wanderson Henrique De Souza Leite, Milane Gonzalez, Sergio Alberto Farber, Marisa Diana Martins, Orlando Da Silva Vaz, Itabajara Oliveira, Pedro L. A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus |
| title | A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus |
| title_full | A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus |
| title_fullStr | A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus |
| title_full_unstemmed | A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus |
| title_short | A Coxiella mutualist symbiont is essential to the development of rhipicephalus microplus |
| title_sort | coxiella mutualist symbiont is essential to the development of rhipicephalus microplus |
| topic | Coxiella Rhipicephalus Metastigmata Simbionte Garrapatas Symbiont Ticks Rhipicephalus microplus |
| url | https://www.nature.com/articles/s41598-017-17309-x http://hdl.handle.net/20.500.12123/3988 https://doi.org/10.1038/s41598-017-17309-x |
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