Redox-related metabolites and gene expression modulated by sugar in sunflower leaves: similarities with Sunflower chlorotic mottle virus-induced symptom
Sugars are part of an integrated redox system, since they are key regulators of respiration and photosynthesis, and therefore of the levels of reducing power, ATP and ROS. These elements are major determinants of the cellular redox state, which is involved in the perception and regulation of many en...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Taylor & Francis
2018
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| Acceso en línea: | https://www.tandfonline.com/doi/full/10.1179/1351000212Y.0000000035 http://hdl.handle.net/20.500.12123/3985 https://doi.org/10.1179/1351000212Y.0000000035 |
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| author | Rodriguez, Marianela Muñoz, Nacira Belen Lenardon, Sergio Luis Lascano, Hernan Ramiro |
| author_browse | Lascano, Hernan Ramiro Lenardon, Sergio Luis Muñoz, Nacira Belen Rodriguez, Marianela |
| author_facet | Rodriguez, Marianela Muñoz, Nacira Belen Lenardon, Sergio Luis Lascano, Hernan Ramiro |
| author_sort | Rodriguez, Marianela |
| collection | INTA Digital |
| description | Sugars are part of an integrated redox system, since they are key regulators of respiration and photosynthesis, and therefore of the levels of reducing power, ATP and ROS. These elements are major determinants of the cellular redox state, which is involved in the perception and regulation of many endogenous and environmental stimuli. Our previous findings suggested that early sugar increase produced during compatible Sunflower chlorotic mottle virus (SuCMoV) infection might modulate chlorotic symptom development through redox state alteration in sunflower. The purpose of this work was to characterize redox-related metabolites and gene expression changes associated with high sugar availability and symptom development induced by SuCMoV. The results show that sugar caused an increase in glutathione, ascorbate, pyridine nucleotides, and ATP. In addition, higher sugar availability reduced hydrogen peroxide and ΦPSII. This finding suggests that high sugar availability would be associated with cellular redox alteration and photoinhibitory process. The expression of the genes analyzed was also strongly affected by sugar, such as the down-regulation of psbA and up-regulation of psbO and cp29. The expression level of cytoplasmic (apx-1 and gr)- and chloroplastic (Fe-sod)-targeted genes was also significantly enhanced in sugar-treated leaves. Therefore, all these responses suggest that sugars induce chloroplastic redox state alteration with photoinhibition process that could be contributing to chlorotic symptom development during SuCMoV infection. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3985 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Taylor & Francis |
| publisherStr | Taylor & Francis |
| record_format | dspace |
| spelling | INTA39852018-11-28T15:25:53Z Redox-related metabolites and gene expression modulated by sugar in sunflower leaves: similarities with Sunflower chlorotic mottle virus-induced symptom Rodriguez, Marianela Muñoz, Nacira Belen Lenardon, Sergio Luis Lascano, Hernan Ramiro Helianthus Annuus Virus de las Plantas Metabolitos Expresión Génica Potencial Redox Azúcar Plant Viruses Metabolites Gene Expression Redox Potential Sugar Girasol Virus del Moteado Clorótico del Girasol Sugars are part of an integrated redox system, since they are key regulators of respiration and photosynthesis, and therefore of the levels of reducing power, ATP and ROS. These elements are major determinants of the cellular redox state, which is involved in the perception and regulation of many endogenous and environmental stimuli. Our previous findings suggested that early sugar increase produced during compatible Sunflower chlorotic mottle virus (SuCMoV) infection might modulate chlorotic symptom development through redox state alteration in sunflower. The purpose of this work was to characterize redox-related metabolites and gene expression changes associated with high sugar availability and symptom development induced by SuCMoV. The results show that sugar caused an increase in glutathione, ascorbate, pyridine nucleotides, and ATP. In addition, higher sugar availability reduced hydrogen peroxide and ΦPSII. This finding suggests that high sugar availability would be associated with cellular redox alteration and photoinhibitory process. The expression of the genes analyzed was also strongly affected by sugar, such as the down-regulation of psbA and up-regulation of psbO and cp29. The expression level of cytoplasmic (apx-1 and gr)- and chloroplastic (Fe-sod)-targeted genes was also significantly enhanced in sugar-treated leaves. Therefore, all these responses suggest that sugars induce chloroplastic redox state alteration with photoinhibition process that could be contributing to chlorotic symptom development during SuCMoV infection. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina 2018-11-28T15:23:49Z 2018-11-28T15:23:49Z 2013 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.tandfonline.com/doi/full/10.1179/1351000212Y.0000000035 http://hdl.handle.net/20.500.12123/3985 1351-0002 1743-2928 https://doi.org/10.1179/1351000212Y.0000000035 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Taylor & Francis Redox Report 18 (1) : 27-35 (2013) |
| spellingShingle | Helianthus Annuus Virus de las Plantas Metabolitos Expresión Génica Potencial Redox Azúcar Plant Viruses Metabolites Gene Expression Redox Potential Sugar Girasol Virus del Moteado Clorótico del Girasol Rodriguez, Marianela Muñoz, Nacira Belen Lenardon, Sergio Luis Lascano, Hernan Ramiro Redox-related metabolites and gene expression modulated by sugar in sunflower leaves: similarities with Sunflower chlorotic mottle virus-induced symptom |
| title | Redox-related metabolites and gene expression modulated by sugar in sunflower leaves: similarities with Sunflower chlorotic mottle virus-induced symptom |
| title_full | Redox-related metabolites and gene expression modulated by sugar in sunflower leaves: similarities with Sunflower chlorotic mottle virus-induced symptom |
| title_fullStr | Redox-related metabolites and gene expression modulated by sugar in sunflower leaves: similarities with Sunflower chlorotic mottle virus-induced symptom |
| title_full_unstemmed | Redox-related metabolites and gene expression modulated by sugar in sunflower leaves: similarities with Sunflower chlorotic mottle virus-induced symptom |
| title_short | Redox-related metabolites and gene expression modulated by sugar in sunflower leaves: similarities with Sunflower chlorotic mottle virus-induced symptom |
| title_sort | redox related metabolites and gene expression modulated by sugar in sunflower leaves similarities with sunflower chlorotic mottle virus induced symptom |
| topic | Helianthus Annuus Virus de las Plantas Metabolitos Expresión Génica Potencial Redox Azúcar Plant Viruses Metabolites Gene Expression Redox Potential Sugar Girasol Virus del Moteado Clorótico del Girasol |
| url | https://www.tandfonline.com/doi/full/10.1179/1351000212Y.0000000035 http://hdl.handle.net/20.500.12123/3985 https://doi.org/10.1179/1351000212Y.0000000035 |
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