Long-term preservation of Lotus tenuis adventitious buds
Encapsulation-dehydration, encapsulation-vitrification, and vitrification were tested for cryopreservation of Lotus tenuis (Fagaceae) adventitious buds clusters (ABCs) obtained by a direct regeneration system from leaves cultures. Among them, the PVS3-based vitrification procedure was found to be us...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/3983 https://link.springer.com/article/10.1007/s11240-018-1522-6 https://doi.org/10.1007/s11240-018-1522-6 |
| _version_ | 1855483342097481728 |
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| author | Espasandin, Fabiana Daniela Brugnoli, Elsa Andrea Ayala, Paula G. Ayala, Lilian P. Ruiz, Oscar Adolfo Sansberro, Pedro Alfonso |
| author_browse | Ayala, Lilian P. Ayala, Paula G. Brugnoli, Elsa Andrea Espasandin, Fabiana Daniela Ruiz, Oscar Adolfo Sansberro, Pedro Alfonso |
| author_facet | Espasandin, Fabiana Daniela Brugnoli, Elsa Andrea Ayala, Paula G. Ayala, Lilian P. Ruiz, Oscar Adolfo Sansberro, Pedro Alfonso |
| author_sort | Espasandin, Fabiana Daniela |
| collection | INTA Digital |
| description | Encapsulation-dehydration, encapsulation-vitrification, and vitrification were tested for cryopreservation of Lotus tenuis (Fagaceae) adventitious buds clusters (ABCs) obtained by a direct regeneration system from leaves cultures. Among them, the PVS3-based vitrification procedure was found to be useful for survival and regrowth of the preserved explants. For vitrification, the ABCs were dehydrated in a solution containing 2 M glycerol + 0.4 M sucrose for 25 min at room temperature, submerged in PVS3 solution for 1 h at 0 °C, then immersed in liquid nitrogen for 48 h and rapidly rewarmed. Afterword, the explants were unloaded in MS liquid medium with 1.2 M sucrose for 30 min. The washed tissues were dried superficially on filter paper and cultured in semisolid hormone-free MS medium containing 0.1 M sucrose. All cultures were maintained at 25 °C in the dark for 10 days and transferred to the light conditions. With this procedure, 79 ± 5.3% survival and more than 80% of the plantlets displaying a phenotype similar to the non-treated control after acclimatization. The data settled from ISSR showed no genetic dissimilarities between in vitro regenerants derived from cryopreserved tissues and the non-treated plants. Thus, our results indicate that the use of vitrification-based PVS3 solution offers a simple, accurate, and appropriate procedure for the cryopreservation of L. tenuis adventitious buds. |
| format | Artículo |
| id | INTA3983 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA39832025-02-19T13:13:08Z Long-term preservation of Lotus tenuis adventitious buds Espasandin, Fabiana Daniela Brugnoli, Elsa Andrea Ayala, Paula G. Ayala, Lilian P. Ruiz, Oscar Adolfo Sansberro, Pedro Alfonso Lotus Tenuis Criopreservación Marcadores Genéticos Vitrificación Yema (planta) Cryopreservation Genetic Markers Vitrification Buds Marcadores Moleculares Encapsulation-dehydration, encapsulation-vitrification, and vitrification were tested for cryopreservation of Lotus tenuis (Fagaceae) adventitious buds clusters (ABCs) obtained by a direct regeneration system from leaves cultures. Among them, the PVS3-based vitrification procedure was found to be useful for survival and regrowth of the preserved explants. For vitrification, the ABCs were dehydrated in a solution containing 2 M glycerol + 0.4 M sucrose for 25 min at room temperature, submerged in PVS3 solution for 1 h at 0 °C, then immersed in liquid nitrogen for 48 h and rapidly rewarmed. Afterword, the explants were unloaded in MS liquid medium with 1.2 M sucrose for 30 min. The washed tissues were dried superficially on filter paper and cultured in semisolid hormone-free MS medium containing 0.1 M sucrose. All cultures were maintained at 25 °C in the dark for 10 days and transferred to the light conditions. With this procedure, 79 ± 5.3% survival and more than 80% of the plantlets displaying a phenotype similar to the non-treated control after acclimatization. The data settled from ISSR showed no genetic dissimilarities between in vitro regenerants derived from cryopreserved tissues and the non-treated plants. Thus, our results indicate that the use of vitrification-based PVS3 solution offers a simple, accurate, and appropriate procedure for the cryopreservation of L. tenuis adventitious buds. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Espasandin, Fabiana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Brugnoli, Elsa Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Ayala, Paula G. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Ayala, Lilian P. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina 2018-11-28T14:33:20Z 2018-11-28T14:33:20Z 2018-11-14 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3983 https://link.springer.com/article/10.1007/s11240-018-1522-6 0167-6857 1573-5044 https://doi.org/10.1007/s11240-018-1522-6 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Plant Cell, Tissue and Organ Culture : 1–10 First Online: 14 November 2018 |
| spellingShingle | Lotus Tenuis Criopreservación Marcadores Genéticos Vitrificación Yema (planta) Cryopreservation Genetic Markers Vitrification Buds Marcadores Moleculares Espasandin, Fabiana Daniela Brugnoli, Elsa Andrea Ayala, Paula G. Ayala, Lilian P. Ruiz, Oscar Adolfo Sansberro, Pedro Alfonso Long-term preservation of Lotus tenuis adventitious buds |
| title | Long-term preservation of Lotus tenuis adventitious buds |
| title_full | Long-term preservation of Lotus tenuis adventitious buds |
| title_fullStr | Long-term preservation of Lotus tenuis adventitious buds |
| title_full_unstemmed | Long-term preservation of Lotus tenuis adventitious buds |
| title_short | Long-term preservation of Lotus tenuis adventitious buds |
| title_sort | long term preservation of lotus tenuis adventitious buds |
| topic | Lotus Tenuis Criopreservación Marcadores Genéticos Vitrificación Yema (planta) Cryopreservation Genetic Markers Vitrification Buds Marcadores Moleculares |
| url | http://hdl.handle.net/20.500.12123/3983 https://link.springer.com/article/10.1007/s11240-018-1522-6 https://doi.org/10.1007/s11240-018-1522-6 |
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