Leptospira species molecular epidemiology in the genomic era
Leptospirosis is a zoonotic disease which global burden is increasing often related to climatic change. Hundreds of whole genome sequences from worldwide isolates of Leptospira spp. are available nowadays, together with online tools that permit to assign MLST sequence types (STs) directly from raw s...
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2018
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S1567134817302708?via%3Dihub http://hdl.handle.net/20.500.12123/3978 http://dx.doi.org/10.1016/j.meegid.2017.08.013 |
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| author | Caimi, Karina Cynthia Repetto, Silvia A. Varni, Vanina Delia Ruybal, Paula |
| author_browse | Caimi, Karina Cynthia Repetto, Silvia A. Ruybal, Paula Varni, Vanina Delia |
| author_facet | Caimi, Karina Cynthia Repetto, Silvia A. Varni, Vanina Delia Ruybal, Paula |
| author_sort | Caimi, Karina Cynthia |
| collection | INTA Digital |
| description | Leptospirosis is a zoonotic disease which global burden is increasing often related to climatic change. Hundreds of whole genome sequences from worldwide isolates of Leptospira spp. are available nowadays, together with online tools that permit to assign MLST sequence types (STs) directly from raw sequence data. In this work we have applied R7L-MLST to near 500 genomes and strains collection globally distributed. All 10 pathogenic species as well as intermediate were typed using this MLST scheme. The correlation observed between STs and serogroups in our previous work, is still satisfied with this higher dataset sustaining the implementation of MLST to assist serological classification as a complementary approach. Bayesian phylogenetic analysis of concatenated sequences from R7-MLST loci allowed us to resolve taxonomic inconsistencies but also showed that events such as recombination, gene conversion or lateral gene transfer played an important role in the evolution of Leptospira genus. Whole genome sequencing allows us to contribute with suitable epidemiologic information useful to apply in the design of control strategies and also in diagnostic methods for this illness. |
| format | Artículo |
| id | INTA3978 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA39782018-11-28T11:03:14Z Leptospira species molecular epidemiology in the genomic era Caimi, Karina Cynthia Repetto, Silvia A. Varni, Vanina Delia Ruybal, Paula Leptospira Genómica Evolución Genomics Evolution Zoonoses Zoonosis MLST Leptospirosis is a zoonotic disease which global burden is increasing often related to climatic change. Hundreds of whole genome sequences from worldwide isolates of Leptospira spp. are available nowadays, together with online tools that permit to assign MLST sequence types (STs) directly from raw sequence data. In this work we have applied R7L-MLST to near 500 genomes and strains collection globally distributed. All 10 pathogenic species as well as intermediate were typed using this MLST scheme. The correlation observed between STs and serogroups in our previous work, is still satisfied with this higher dataset sustaining the implementation of MLST to assist serological classification as a complementary approach. Bayesian phylogenetic analysis of concatenated sequences from R7-MLST loci allowed us to resolve taxonomic inconsistencies but also showed that events such as recombination, gene conversion or lateral gene transfer played an important role in the evolution of Leptospira genus. Whole genome sequencing allows us to contribute with suitable epidemiologic information useful to apply in the design of control strategies and also in diagnostic methods for this illness. Instituto de Biotecnología Fil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Repetto, Silvia A. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Microbiología y Parasitología Médica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Varni, Vanina Delia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ruybal, Paula. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Microbiología y Parasitología Médica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-11-28T10:56:20Z 2018-11-28T10:56:20Z 2017-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S1567134817302708?via%3Dihub http://hdl.handle.net/20.500.12123/3978 1567-1348 http://dx.doi.org/10.1016/j.meegid.2017.08.013 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Infection, Genetics and Evolution 54 : 478-485 (Octubre 2017) |
| spellingShingle | Leptospira Genómica Evolución Genomics Evolution Zoonoses Zoonosis MLST Caimi, Karina Cynthia Repetto, Silvia A. Varni, Vanina Delia Ruybal, Paula Leptospira species molecular epidemiology in the genomic era |
| title | Leptospira species molecular epidemiology in the genomic era |
| title_full | Leptospira species molecular epidemiology in the genomic era |
| title_fullStr | Leptospira species molecular epidemiology in the genomic era |
| title_full_unstemmed | Leptospira species molecular epidemiology in the genomic era |
| title_short | Leptospira species molecular epidemiology in the genomic era |
| title_sort | leptospira species molecular epidemiology in the genomic era |
| topic | Leptospira Genómica Evolución Genomics Evolution Zoonoses Zoonosis MLST |
| url | https://www.sciencedirect.com/science/article/pii/S1567134817302708?via%3Dihub http://hdl.handle.net/20.500.12123/3978 http://dx.doi.org/10.1016/j.meegid.2017.08.013 |
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