Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay
Antigens with aromatic amino groups can be conjugated to proteins by a diazo bond. This property was used to synthesise an antigen-biotin conjugate using bovine serum albumin (BSA) as a bridge, and tested this conjugate in an enzymoimmunoassay for clenbuterol detection. The assay included a highly s...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Taylor & Francis
2018
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| Online Access: | https://www.tandfonline.com/doi/full/10.1080/09540105.2011.629314 http://hdl.handle.net/20.500.12123/3977 https://doi.org/10.1080/09540105.2011.629314 |
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| author | Spotorno, Viviana Graciela Tezón, Jorge Guillermo |
| author_browse | Spotorno, Viviana Graciela Tezón, Jorge Guillermo |
| author_facet | Spotorno, Viviana Graciela Tezón, Jorge Guillermo |
| author_sort | Spotorno, Viviana Graciela |
| collection | INTA Digital |
| description | Antigens with aromatic amino groups can be conjugated to proteins by a diazo bond. This property was used to synthesise an antigen-biotin conjugate using bovine serum albumin (BSA) as a bridge, and tested this conjugate in an enzymoimmunoassay for clenbuterol detection. The assay included a highly specific antibody and the biotin-avidin amplification system. Its coefficient of variation was lower than 20% in the range of 100–8000 pg per well and the limit of detection was 6.4 pg. The synthesis of clenbuterol–BSA–biotin conjugate was reproducible, the reaction product easily purified and the amplification capacity could be set by adjusting the clenbuterol:biotin ratio |
| format | info:ar-repo/semantics/artículo |
| id | INTA3977 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Taylor & Francis |
| publisherStr | Taylor & Francis |
| record_format | dspace |
| spelling | INTA39772018-11-27T14:53:29Z Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay Spotorno, Viviana Graciela Tezón, Jorge Guillermo Técnicas Inmunológicas Antígenos Biotina Técnicas Inmunoenzimáticas Clenbuterol Immunological Techniques Antigens Biotin Immunoenzyme Techniques Inmunoensayos Antigens with aromatic amino groups can be conjugated to proteins by a diazo bond. This property was used to synthesise an antigen-biotin conjugate using bovine serum albumin (BSA) as a bridge, and tested this conjugate in an enzymoimmunoassay for clenbuterol detection. The assay included a highly specific antibody and the biotin-avidin amplification system. Its coefficient of variation was lower than 20% in the range of 100–8000 pg per well and the limit of detection was 6.4 pg. The synthesis of clenbuterol–BSA–biotin conjugate was reproducible, the reaction product easily purified and the amplification capacity could be set by adjusting the clenbuterol:biotin ratio Instituto de Tecnología de Alimentos Fil: Spotorno, Viviana Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Tecnología de Alimentos; Argentina Fil: Tezon, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina 2018-11-27T14:44:34Z 2018-11-27T14:44:34Z 2012-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.tandfonline.com/doi/full/10.1080/09540105.2011.629314 http://hdl.handle.net/20.500.12123/3977 0954-0105 1465-3443 https://doi.org/10.1080/09540105.2011.629314 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Taylor & Francis Food and Agricultural Immunology 23 (4) : 315-328 (December 2012) |
| spellingShingle | Técnicas Inmunológicas Antígenos Biotina Técnicas Inmunoenzimáticas Clenbuterol Immunological Techniques Antigens Biotin Immunoenzyme Techniques Inmunoensayos Spotorno, Viviana Graciela Tezón, Jorge Guillermo Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay |
| title | Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay |
| title_full | Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay |
| title_fullStr | Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay |
| title_full_unstemmed | Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay |
| title_short | Development of an antigen-biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay |
| title_sort | development of an antigen biotin conjugate with a protein bridge and its application for the development of an enzyme immunoassay |
| topic | Técnicas Inmunológicas Antígenos Biotina Técnicas Inmunoenzimáticas Clenbuterol Immunological Techniques Antigens Biotin Immunoenzyme Techniques Inmunoensayos |
| url | https://www.tandfonline.com/doi/full/10.1080/09540105.2011.629314 http://hdl.handle.net/20.500.12123/3977 https://doi.org/10.1080/09540105.2011.629314 |
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