Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation
Despite Neospora caninum being a major cause of bovine abortion worldwide, its pathogenesis is not completely understood. Neospora infection stimulates host cell-mediated immune responses, which may be responsible for the placental damage leading to abortion. The aim of the current study was to char...
| Autores principales: | , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
BMC
2018
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| Materias: | |
| Acceso en línea: | https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-44-60 http://hdl.handle.net/20.500.12123/3948 https://doi.org/10.1186/1297-9716-44-60 |
| _version_ | 1855035233215184896 |
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| author | Canton, German Jose Katzer, Frank Benavides-Silván, Julio Maley, Stephen W. Palarea-Albaladejo, Javier Pang, Yvonne Smith, Sionagh Bartley, Paul M. Rocchi, Mara Innes, Elisabeth A. Chianini, Francesca |
| author_browse | Bartley, Paul M. Benavides-Silván, Julio Canton, German Jose Chianini, Francesca Innes, Elisabeth A. Katzer, Frank Maley, Stephen W. Palarea-Albaladejo, Javier Pang, Yvonne Rocchi, Mara Smith, Sionagh |
| author_facet | Canton, German Jose Katzer, Frank Benavides-Silván, Julio Maley, Stephen W. Palarea-Albaladejo, Javier Pang, Yvonne Smith, Sionagh Bartley, Paul M. Rocchi, Mara Innes, Elisabeth A. Chianini, Francesca |
| author_sort | Canton, German Jose |
| collection | INTA Digital |
| description | Despite Neospora caninum being a major cause of bovine abortion worldwide, its pathogenesis is not completely understood. Neospora infection stimulates host cell-mediated immune responses, which may be responsible for the placental damage leading to abortion. The aim of the current study was to characterize the placental immune response following an experimental inoculation of pregnant cattle with N. caninum tachyzoites at day 210 of gestation. Cows were culled at 14, 28, 42 and 56 days post inoculation (dpi). Placentomes were examined by immunohistochemistry using antibodies against macrophages, T-cell subsets (CD4, CD8 and γδ), NK cells and B cells. Macrophages were detected mainly at 14 days post inoculation. Inflammation was generally mild and mainly characterized by CD3+, CD4+ and γδ T-cells; whereas CD8+ and NK cells were less numerous. The immune cell repertoire observed in this study was similar to those seen in pregnant cattle challenged with N. caninum at early gestation. However, cellular infiltrates were less severe than those seen during first trimester Neospora infections. This may explain the milder clinical outcome observed when animals are infected late in gestation. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3948 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | BMC |
| publisherStr | BMC |
| record_format | dspace |
| spelling | INTA39482018-11-22T14:45:35Z Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation Canton, German Jose Katzer, Frank Benavides-Silván, Julio Maley, Stephen W. Palarea-Albaladejo, Javier Pang, Yvonne Smith, Sionagh Bartley, Paul M. Rocchi, Mara Innes, Elisabeth A. Chianini, Francesca Neospora caninum Ganado Bovino Placenta Fenotipos Gestación Inoculación Cattle Phenotypes Pregnancy Inoculation Despite Neospora caninum being a major cause of bovine abortion worldwide, its pathogenesis is not completely understood. Neospora infection stimulates host cell-mediated immune responses, which may be responsible for the placental damage leading to abortion. The aim of the current study was to characterize the placental immune response following an experimental inoculation of pregnant cattle with N. caninum tachyzoites at day 210 of gestation. Cows were culled at 14, 28, 42 and 56 days post inoculation (dpi). Placentomes were examined by immunohistochemistry using antibodies against macrophages, T-cell subsets (CD4, CD8 and γδ), NK cells and B cells. Macrophages were detected mainly at 14 days post inoculation. Inflammation was generally mild and mainly characterized by CD3+, CD4+ and γδ T-cells; whereas CD8+ and NK cells were less numerous. The immune cell repertoire observed in this study was similar to those seen in pregnant cattle challenged with N. caninum at early gestation. However, cellular infiltrates were less severe than those seen during first trimester Neospora infections. This may explain the milder clinical outcome observed when animals are infected late in gestation. EEA Balcarce Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Katzer, Frank. Moredun Research Institute; Reino Unido Fil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; España Fil: Maley, Stephen W. Moredun Research Institute; Reino Unido Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido Fil: Pang, Yvonne. Moredun Research Institute; Reino Unido Fil: Smith, Sionagh. University of Edinburgh. Royal (Dick) School of Veterinary Studies and Roslin Institute; Reino Unido Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido Fil: Rocchi, Mara. Moredun Research Institute; Reino Unido Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido 2018-11-22T14:44:14Z 2018-11-22T14:44:14Z 2013-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-44-60 http://hdl.handle.net/20.500.12123/3948 0928-4249 1297-9716 https://doi.org/10.1186/1297-9716-44-60 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BMC Veterinary Research 44 : 60 (2013) |
| spellingShingle | Neospora caninum Ganado Bovino Placenta Fenotipos Gestación Inoculación Cattle Phenotypes Pregnancy Inoculation Canton, German Jose Katzer, Frank Benavides-Silván, Julio Maley, Stephen W. Palarea-Albaladejo, Javier Pang, Yvonne Smith, Sionagh Bartley, Paul M. Rocchi, Mara Innes, Elisabeth A. Chianini, Francesca Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation |
| title | Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation |
| title_full | Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation |
| title_fullStr | Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation |
| title_full_unstemmed | Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation |
| title_short | Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation |
| title_sort | phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with neospora caninum in late gestation |
| topic | Neospora caninum Ganado Bovino Placenta Fenotipos Gestación Inoculación Cattle Phenotypes Pregnancy Inoculation |
| url | https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-44-60 http://hdl.handle.net/20.500.12123/3948 https://doi.org/10.1186/1297-9716-44-60 |
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