Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method for the identification of this microorganism was designed ba...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/3935 https://www.sciencedirect.com/science/article/pii/S0034528811000853 https://doi.org/10.1016/j.rvsc.2011.02.007 |
| _version_ | 1855483333218140160 |
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| author | Pavan, María Elisa Robles, Carlos Alejandro Cairó, Fabián A. Marcellino, Romanela Pettinari, María Julia |
| author_browse | Cairó, Fabián A. Marcellino, Romanela Pavan, María Elisa Pettinari, María Julia Robles, Carlos Alejandro |
| author_facet | Pavan, María Elisa Robles, Carlos Alejandro Cairó, Fabián A. Marcellino, Romanela Pettinari, María Julia |
| author_sort | Pavan, María Elisa |
| collection | INTA Digital |
| description | Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method for the identification of this microorganism was designed based on the hypervariable region of the polymorphic RNA polymerase b-subunit gene (rpoB). All available Corynebacterium rpoB sequences were analyzed by computer-assisted restriction analysis. The rpoB
PCR–RFLP pattern predicted by using endonucleases MseI and StuI clearly differentiated C. pseudotuberculosis from sixty-one other Corynebacterium species. This method was successfully applied to identify twelve wild C. pseudotuberculosis ovine isolates and one caprine isolate. It was also used to differentiate C. pseudotuberculosis from Arcanobacterium pyogenes, an ovine pathogen with similar clinical characteristics. These results indicate that this new molecular method can be used for the reliable identification of
the pathogen, essential for the timely detection of infected animals and for epidemiological studies. |
| format | Artículo |
| id | INTA3935 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA39352018-11-21T12:55:38Z Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB) Pavan, María Elisa Robles, Carlos Alejandro Cairó, Fabián A. Marcellino, Romanela Pettinari, María Julia Corynebacterium Sanidad Animal Ovinos Animal Health Sheep Caseous Lymphadenitis Identification PCR Linfoadenitis Caseosa Corynebacterium pseudotuberculosis Identificación Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method for the identification of this microorganism was designed based on the hypervariable region of the polymorphic RNA polymerase b-subunit gene (rpoB). All available Corynebacterium rpoB sequences were analyzed by computer-assisted restriction analysis. The rpoB PCR–RFLP pattern predicted by using endonucleases MseI and StuI clearly differentiated C. pseudotuberculosis from sixty-one other Corynebacterium species. This method was successfully applied to identify twelve wild C. pseudotuberculosis ovine isolates and one caprine isolate. It was also used to differentiate C. pseudotuberculosis from Arcanobacterium pyogenes, an ovine pathogen with similar clinical characteristics. These results indicate that this new molecular method can be used for the reliable identification of the pathogen, essential for the timely detection of infected animals and for epidemiological studies. Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo de Salud Animal Fil: Paván, María Elisa. Biochemiq S.A. Laboratorio de Biología Molecular; Argentina Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo Salud Animal; Argentina Fil: Cairó, Fabián A. Biochemiq S.A. Laboratorio de Biología Molecular; Argentina Fil: Marcellino, Romanela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo Salud Animal; Argentina FiL: Pettinari, María Julia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina 2018-11-21T12:42:40Z 2018-11-21T12:42:40Z 2012-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3935 https://www.sciencedirect.com/science/article/pii/S0034528811000853 0034-5288 https://doi.org/10.1016/j.rvsc.2011.02.007 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Research in Veterinary Science 92 : 202–206 (2012) |
| spellingShingle | Corynebacterium Sanidad Animal Ovinos Animal Health Sheep Caseous Lymphadenitis Identification PCR Linfoadenitis Caseosa Corynebacterium pseudotuberculosis Identificación Pavan, María Elisa Robles, Carlos Alejandro Cairó, Fabián A. Marcellino, Romanela Pettinari, María Julia Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB) |
| title | Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB) |
| title_full | Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB) |
| title_fullStr | Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB) |
| title_full_unstemmed | Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB) |
| title_short | Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB) |
| title_sort | identification of corynebacterium pseudotuberculosis from sheep by pcr restriction analysis using the rna polymerase β subunit gene rpob |
| topic | Corynebacterium Sanidad Animal Ovinos Animal Health Sheep Caseous Lymphadenitis Identification PCR Linfoadenitis Caseosa Corynebacterium pseudotuberculosis Identificación |
| url | http://hdl.handle.net/20.500.12123/3935 https://www.sciencedirect.com/science/article/pii/S0034528811000853 https://doi.org/10.1016/j.rvsc.2011.02.007 |
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