Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis
Onion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determinati...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Royal Society of Chemistry
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/3905 https://pubs.rsc.org/en/content/articlelanding/2014/ay/c4ay00240g#!divAbstract |
| _version_ | 1855035225540657152 |
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| author | Soto Vargas, Verónica Carolina Fernández, María de Los Ángeles Galmarini, Claudio Romulo Silva, María Fernanda |
| author_browse | Fernández, María de Los Ángeles Galmarini, Claudio Romulo Silva, María Fernanda Soto Vargas, Verónica Carolina |
| author_facet | Soto Vargas, Verónica Carolina Fernández, María de Los Ángeles Galmarini, Claudio Romulo Silva, María Fernanda |
| author_sort | Soto Vargas, Verónica Carolina |
| collection | INTA Digital |
| description | Onion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determination of 4-vinylphenol, catechin, naringenin, rutin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in onion nectar lines by CZE-UV is reported. Limited nectar amounts make it difficult to apply the most commonly employed methods of extraction for phenolic compounds. Thus, the extraction of phenolic compounds in nectar was performed by solid phase extraction (SPE) using a home-made minicolumn packed with a suitable filtering material (C18, 50 mg) connected to a vacuum pump. Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire the optimum conditions. Under the proposed conditions, the analytes could be separated within 8 min in a 50 cm effective length capillary (75 μm ID) at a separation voltage of 25 kV in 30 mmol L−1 boric acid as BGE (pH 9.5). Sample results suggest that the phenolic composition has great influence on bee preference. Furthermore, the markedly qualitative and quantitative analytical differences in the phenolic profile of onion lines found in this study contribute to the understanding of the factors that affect onion pollination for hybrid seed production. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3905 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Royal Society of Chemistry |
| publisherStr | Royal Society of Chemistry |
| record_format | dspace |
| spelling | INTA39052018-11-15T15:04:28Z Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis Soto Vargas, Verónica Carolina Fernández, María de Los Ángeles Galmarini, Claudio Romulo Silva, María Fernanda Compuestos Fenólicos Néctar Cebolla Allium Cepa Electroforesis Phenolic Compounds Onions Electrophoresis Capillary Electrophoresis Solid Phase Extraction SPE Onion is an important vegetable crop which depends heavily on cross-pollinating insects for any significant increase in seed production. Nectar is the most important floral reward offered by plants. Minor components such as phenolic compounds in nectar affect bee foraging. A simultaneous determination of 4-vinylphenol, catechin, naringenin, rutin, cinnamic acid, syringic acid, chlorogenic acid, apigenin, vanillic acid, luteolin, quercetin, and caffeic acid in onion nectar lines by CZE-UV is reported. Limited nectar amounts make it difficult to apply the most commonly employed methods of extraction for phenolic compounds. Thus, the extraction of phenolic compounds in nectar was performed by solid phase extraction (SPE) using a home-made minicolumn packed with a suitable filtering material (C18, 50 mg) connected to a vacuum pump. Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire the optimum conditions. Under the proposed conditions, the analytes could be separated within 8 min in a 50 cm effective length capillary (75 μm ID) at a separation voltage of 25 kV in 30 mmol L−1 boric acid as BGE (pH 9.5). Sample results suggest that the phenolic composition has great influence on bee preference. Furthermore, the markedly qualitative and quantitative analytical differences in the phenolic profile of onion lines found in this study contribute to the understanding of the factors that affect onion pollination for hybrid seed production. EEA La Consulta Fil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Fernández, María de Los Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Galmarini, Claudio Romulo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina 2018-11-15T14:41:54Z 2018-11-15T14:41:54Z 2014 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3905 https://pubs.rsc.org/en/content/articlelanding/2014/ay/c4ay00240g#!divAbstract 1759-9679 10.1039/c4ay00240g eng info:eu-repo/semantics/restrictedAccess application/pdf Royal Society of Chemistry Analytical methods 13 : 4878–4884. (2014) |
| spellingShingle | Compuestos Fenólicos Néctar Cebolla Allium Cepa Electroforesis Phenolic Compounds Onions Electrophoresis Capillary Electrophoresis Solid Phase Extraction SPE Soto Vargas, Verónica Carolina Fernández, María de Los Ángeles Galmarini, Claudio Romulo Silva, María Fernanda Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis |
| title | Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis |
| title_full | Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis |
| title_fullStr | Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis |
| title_full_unstemmed | Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis |
| title_short | Analysis of phenolic compounds in onion nectar by miniaturized off-line solid phase extraction-capillary zone electrophoresis |
| title_sort | analysis of phenolic compounds in onion nectar by miniaturized off line solid phase extraction capillary zone electrophoresis |
| topic | Compuestos Fenólicos Néctar Cebolla Allium Cepa Electroforesis Phenolic Compounds Onions Electrophoresis Capillary Electrophoresis Solid Phase Extraction SPE |
| url | http://hdl.handle.net/20.500.12123/3905 https://pubs.rsc.org/en/content/articlelanding/2014/ay/c4ay00240g#!divAbstract |
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