Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster
The range of thermal tolerance is one of the main factors influencing the geographic distribution of species. Climate change projections predict increases in average and extreme temperatures over the coming decades; hence, the ability of living beings to resist these changes will depend on physiolo...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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John Wiley & Sons Ltd
2018
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| Online Access: | http://hdl.handle.net/20.500.12123/3860 https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.4409 https://doi.org/10.1002/ece3.4409 |
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| author | Rolandi, Carmen Lighton, John R.B. De La Vega, Gerardo Schilman, Pablo Ernesto Mensch, Julián |
| author_browse | De La Vega, Gerardo Lighton, John R.B. Mensch, Julián Rolandi, Carmen Schilman, Pablo Ernesto |
| author_facet | Rolandi, Carmen Lighton, John R.B. De La Vega, Gerardo Schilman, Pablo Ernesto Mensch, Julián |
| author_sort | Rolandi, Carmen |
| collection | INTA Digital |
| description | The range of thermal tolerance is one of the main factors influencing the geographic distribution of species. Climate change projections predict increases in average and extreme temperatures over the coming decades; hence, the ability of living beings to
resist these changes will depend on physiological and adaptive responses. On an evolutionary scale, changes will occur as the result of selective pressures on individual heritable differences. In this work, we studied the genetic basis of tolerance to high temperatures in the fly Drosophila melanogaster and whether this species presents sufficient genetic variability to allow expansion of its upper thermo-tolerance limit.
To do so, we used adult flies derived from a natural population belonging to the Drosophila Genetic Reference Panel, for which genomic sequencing data are available. We characterized the phenotypic variation of the upper thermal limit in 34 lines by measuring knockdown temperature (i.e., critical thermal maximum [CTmax]) by exposing flies to a ramp of increasing temperature (0.25°C/min). Fourteen percent of the variation in CTmax is explained by the genetic variation across lines, without a significant sexual dimorphism. Through a genomewide association study, 12 single nucleotide polymorphisms associated with the CTmax were identified. In most of
these SNPs, the less frequent allele increased the upper thermal limit suggesting that this population harbors raw genetic variation capable of expanding its heat tolerance. This potential upper thermal tolerance increase has implications under the global
warming scenario. Past climatic records show a very low incidence of days above CTmax (10 days over 25 years); however, future climate scenarios predict 243 days with extreme high temperature above CTmax from 2045 to 2070. Thus, in the context of the future climate warming, rising temperatures might drive the evolution of heat tolerance in this population by increasing the frequency of the alleles associated with higher CTmax. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3860 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | John Wiley & Sons Ltd |
| publisherStr | John Wiley & Sons Ltd |
| record_format | dspace |
| spelling | INTA38602018-11-12T12:37:17Z Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster Rolandi, Carmen Lighton, John R.B. De La Vega, Gerardo Schilman, Pablo Ernesto Mensch, Julián Drosophila Variación Genética Cambio Climático Genetic Variation Climate Change Temperature Heat Tolerance Drosophila melanogaster Temperatura Tolerancia al Calor Moscas The range of thermal tolerance is one of the main factors influencing the geographic distribution of species. Climate change projections predict increases in average and extreme temperatures over the coming decades; hence, the ability of living beings to resist these changes will depend on physiological and adaptive responses. On an evolutionary scale, changes will occur as the result of selective pressures on individual heritable differences. In this work, we studied the genetic basis of tolerance to high temperatures in the fly Drosophila melanogaster and whether this species presents sufficient genetic variability to allow expansion of its upper thermo-tolerance limit. To do so, we used adult flies derived from a natural population belonging to the Drosophila Genetic Reference Panel, for which genomic sequencing data are available. We characterized the phenotypic variation of the upper thermal limit in 34 lines by measuring knockdown temperature (i.e., critical thermal maximum [CTmax]) by exposing flies to a ramp of increasing temperature (0.25°C/min). Fourteen percent of the variation in CTmax is explained by the genetic variation across lines, without a significant sexual dimorphism. Through a genomewide association study, 12 single nucleotide polymorphisms associated with the CTmax were identified. In most of these SNPs, the less frequent allele increased the upper thermal limit suggesting that this population harbors raw genetic variation capable of expanding its heat tolerance. This potential upper thermal tolerance increase has implications under the global warming scenario. Past climatic records show a very low incidence of days above CTmax (10 days over 25 years); however, future climate scenarios predict 243 days with extreme high temperature above CTmax from 2045 to 2070. Thus, in the context of the future climate warming, rising temperatures might drive the evolution of heat tolerance in this population by increasing the frequency of the alleles associated with higher CTmax. Estación Experimental Agropecuaria Bariloche Fil: Rolandi, Carmen. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Lighton, John R. Sable Systems International; Estados Unidos Fil: De la Vega, Gerardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Ecología de Poblaciones de Insectos; Argentina Fil: Schilman, Pablo Ernesto. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Mensch, Julián. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina 2018-11-12T12:26:47Z 2018-11-12T12:26:47Z 2018-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3860 https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.4409 2045-7758 https://doi.org/10.1002/ece3.4409 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf John Wiley & Sons Ltd Ecology and Evolution 8 (20) : 1-10 (2018) |
| spellingShingle | Drosophila Variación Genética Cambio Climático Genetic Variation Climate Change Temperature Heat Tolerance Drosophila melanogaster Temperatura Tolerancia al Calor Moscas Rolandi, Carmen Lighton, John R.B. De La Vega, Gerardo Schilman, Pablo Ernesto Mensch, Julián Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster |
| title | Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster |
| title_full | Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster |
| title_fullStr | Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster |
| title_full_unstemmed | Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster |
| title_short | Genetic variation for tolerance to high temperatures in a population of Drosophila melanogaster |
| title_sort | genetic variation for tolerance to high temperatures in a population of drosophila melanogaster |
| topic | Drosophila Variación Genética Cambio Climático Genetic Variation Climate Change Temperature Heat Tolerance Drosophila melanogaster Temperatura Tolerancia al Calor Moscas |
| url | http://hdl.handle.net/20.500.12123/3860 https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.4409 https://doi.org/10.1002/ece3.4409 |
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