Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina
Brucellosis is a zoonotic disease transmitted from an animal reservoir to humans. Both, wildlife and domestic animals, contribute to the spreading of these zoonosis. The surveillance of the animal health status is strictly regulated for domestic animals, whereas disease monitoring in wildlife does n...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2018
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| Online Access: | http://hdl.handle.net/20.500.12123/3847 https://www.sciencedirect.com/science/article/pii/S0378113514000741?via%3Dihub https://doi.org/10.1016/j.vetmic.2014.01.039 |
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| author | Kin, Marta Susana Fort, Marcelo Cristian Torioni, Susana Marta Casanave, Emma Beatriz |
| author_browse | Casanave, Emma Beatriz Fort, Marcelo Cristian Kin, Marta Susana Torioni, Susana Marta |
| author_facet | Kin, Marta Susana Fort, Marcelo Cristian Torioni, Susana Marta Casanave, Emma Beatriz |
| author_sort | Kin, Marta Susana |
| collection | INTA Digital |
| description | Brucellosis is a zoonotic disease transmitted from an animal reservoir to humans. Both, wildlife and domestic animals, contribute to the spreading of these zoonosis. The surveillance of the animal health status is strictly regulated for domestic animals, whereas disease monitoring in wildlife does not exist. The aim of the present study was to provide data on the prevalence of anti-Brucella antibodies in Chaetophractus villosus from a region of La Pampa, Argentina to assess public health risks. The C. villosus is endemic to South America, and in Argentina it represents a food resource for human consumption. A total of 150 sera of armadillos bleeding between 2007 and 2010 were tested using buffered plate antigen test (BPAT), serum agglutination test (SAT), 2-mercaptoethanol (2-ME) and complement fixation test (CFT), for the detection of anti-Brucella antibodies. Antibodies to Brucella sp. were found in 16% (24:150) of the armadillos tested using the BPAT test. All 24 positive samples were confirmed by the SAT, 2-ME and CFT tests. Strain isolation was attempted from liver and spleen samples of two animals with positive serology. Isolates were characterized by conventional biotyping and identification of specific DNA using polymerase chain reaction (PCR). A total of 2 isolates were recovered from spleen and liver. Both of them were identified as Brucella suis biovar 1. This preliminary study provides the first report on the seroprevalence of brucellosis and describes the first isolate of B. suis biovar 1 in C. villosus in Argentina. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3847 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA38472018-11-09T13:52:04Z Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina Kin, Marta Susana Fort, Marcelo Cristian Torioni, Susana Marta Casanave, Emma Beatriz Brucella Suis Armadillo Xenarthra zoonosis Anticuerpos Zoonoses Antibodies Chaetophractus Villosus La Pampa Brucellosis is a zoonotic disease transmitted from an animal reservoir to humans. Both, wildlife and domestic animals, contribute to the spreading of these zoonosis. The surveillance of the animal health status is strictly regulated for domestic animals, whereas disease monitoring in wildlife does not exist. The aim of the present study was to provide data on the prevalence of anti-Brucella antibodies in Chaetophractus villosus from a region of La Pampa, Argentina to assess public health risks. The C. villosus is endemic to South America, and in Argentina it represents a food resource for human consumption. A total of 150 sera of armadillos bleeding between 2007 and 2010 were tested using buffered plate antigen test (BPAT), serum agglutination test (SAT), 2-mercaptoethanol (2-ME) and complement fixation test (CFT), for the detection of anti-Brucella antibodies. Antibodies to Brucella sp. were found in 16% (24:150) of the armadillos tested using the BPAT test. All 24 positive samples were confirmed by the SAT, 2-ME and CFT tests. Strain isolation was attempted from liver and spleen samples of two animals with positive serology. Isolates were characterized by conventional biotyping and identification of specific DNA using polymerase chain reaction (PCR). A total of 2 isolates were recovered from spleen and liver. Both of them were identified as Brucella suis biovar 1. This preliminary study provides the first report on the seroprevalence of brucellosis and describes the first isolate of B. suis biovar 1 in C. villosus in Argentina. EEA Anguil Fil: Kin, Marta Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Fort, Marcelo Cristian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Torioni, Susana Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Casanave, Emma Beatriz. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico Bahia Blanca. Instituto de Ciencias Biologicas y Biomedicas del Sur; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina 2018-11-09T13:47:03Z 2018-11-09T13:47:03Z 2014-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3847 https://www.sciencedirect.com/science/article/pii/S0378113514000741?via%3Dihub 0378-1135 https://doi.org/10.1016/j.vetmic.2014.01.039 eng info:eu-repo/semantics/restrictedAccess application/pdf La Pampa (province) Elsevier Veterinary microbiology 170 (3–4) : 442-445. (4 June 2014) |
| spellingShingle | Brucella Suis Armadillo Xenarthra zoonosis Anticuerpos Zoonoses Antibodies Chaetophractus Villosus La Pampa Kin, Marta Susana Fort, Marcelo Cristian Torioni, Susana Marta Casanave, Emma Beatriz Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina |
| title | Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina |
| title_full | Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina |
| title_fullStr | Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina |
| title_full_unstemmed | Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina |
| title_short | Brucella suis in armadillos (Chaetophractus villosus) from La Pampa, Argentina |
| title_sort | brucella suis in armadillos chaetophractus villosus from la pampa argentina |
| topic | Brucella Suis Armadillo Xenarthra zoonosis Anticuerpos Zoonoses Antibodies Chaetophractus Villosus La Pampa |
| url | http://hdl.handle.net/20.500.12123/3847 https://www.sciencedirect.com/science/article/pii/S0378113514000741?via%3Dihub https://doi.org/10.1016/j.vetmic.2014.01.039 |
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