Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses
We have determined the full-length 14,491-nucleotide genome sequence of a new plant rhabdovirus, alfalfa dwarf virus (ADV). Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3′-N-P-P3-M-G-P6-L-5′. The ORFs a...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/3803 https://www.sciencedirect.com/science/article/pii/S0042682215002457?via%3Dihub https://doi.org/10.1016/j.virol.2015.05.001 |
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| author | Bejerman, Nicolas Esteban Giolitti, Fabian De Breuil, Soledad Trucco, Veronica Milagros Nome Docampo, Claudia Lenardon, Sergio Luis Dietzgen, Ralf Georg |
| author_browse | Bejerman, Nicolas Esteban De Breuil, Soledad Dietzgen, Ralf Georg Giolitti, Fabian Lenardon, Sergio Luis Nome Docampo, Claudia Trucco, Veronica Milagros |
| author_facet | Bejerman, Nicolas Esteban Giolitti, Fabian De Breuil, Soledad Trucco, Veronica Milagros Nome Docampo, Claudia Lenardon, Sergio Luis Dietzgen, Ralf Georg |
| author_sort | Bejerman, Nicolas Esteban |
| collection | INTA Digital |
| description | We have determined the full-length 14,491-nucleotide genome sequence of a new plant rhabdovirus, alfalfa dwarf virus (ADV). Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3′-N-P-P3-M-G-P6-L-5′. The ORFs are separated by conserved intergenic regions and the genome coding region is flanked by complementary 3′ leader and 5′ trailer sequences. Phylogenetic analysis of the nucleoprotein amino acid sequence indicated that this alfalfa-infecting rhabdovirus is related to viruses in the genus Cytorhabdovirus. When transiently expressed as GFP fusions in Nicotiana benthamiana leaves, most ADV proteins accumulated in the cell periphery, but unexpectedly P protein was localized exclusively in the nucleus. ADV P protein was shown to have a homotypic, and heterotypic nuclear interactions with N, P3 and M proteins by bimolecular fluorescence complementation. ADV appears unique in that it combines properties of both cytoplasmic and nuclear plant rhabdoviruses. |
| format | Artículo |
| id | INTA3803 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA38032018-11-06T18:08:39Z Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses Bejerman, Nicolas Esteban Giolitti, Fabian De Breuil, Soledad Trucco, Veronica Milagros Nome Docampo, Claudia Lenardon, Sergio Luis Dietzgen, Ralf Georg Medicago Sativa Genomas Cytorhabdovirus Nucleorhabdovirus Proteínas Fosfoproteínas Genomes Proteins Phosphoproteins Alfalfa Rabdovirus We have determined the full-length 14,491-nucleotide genome sequence of a new plant rhabdovirus, alfalfa dwarf virus (ADV). Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3′-N-P-P3-M-G-P6-L-5′. The ORFs are separated by conserved intergenic regions and the genome coding region is flanked by complementary 3′ leader and 5′ trailer sequences. Phylogenetic analysis of the nucleoprotein amino acid sequence indicated that this alfalfa-infecting rhabdovirus is related to viruses in the genus Cytorhabdovirus. When transiently expressed as GFP fusions in Nicotiana benthamiana leaves, most ADV proteins accumulated in the cell periphery, but unexpectedly P protein was localized exclusively in the nucleus. ADV P protein was shown to have a homotypic, and heterotypic nuclear interactions with N, P3 and M proteins by bimolecular fluorescence complementation. ADV appears unique in that it combines properties of both cytoplasmic and nuclear plant rhabdoviruses. Instituto de Patología Vegetal Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Trucco, Veronica Milagros: Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Dietzgen, Ralf Georg. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia 2018-11-06T18:04:21Z 2018-11-06T18:04:21Z 2015-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3803 https://www.sciencedirect.com/science/article/pii/S0042682215002457?via%3Dihub 0042-6822 https://doi.org/10.1016/j.virol.2015.05.001 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Virology 483 : 275-283. (September 2015) |
| spellingShingle | Medicago Sativa Genomas Cytorhabdovirus Nucleorhabdovirus Proteínas Fosfoproteínas Genomes Proteins Phosphoproteins Alfalfa Rabdovirus Bejerman, Nicolas Esteban Giolitti, Fabian De Breuil, Soledad Trucco, Veronica Milagros Nome Docampo, Claudia Lenardon, Sergio Luis Dietzgen, Ralf Georg Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses |
| title | Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses |
| title_full | Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses |
| title_fullStr | Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses |
| title_full_unstemmed | Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses |
| title_short | Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses |
| title_sort | complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus which combines properties of both cytoplasmic and nuclear plant rhabdoviruses |
| topic | Medicago Sativa Genomas Cytorhabdovirus Nucleorhabdovirus Proteínas Fosfoproteínas Genomes Proteins Phosphoproteins Alfalfa Rabdovirus |
| url | http://hdl.handle.net/20.500.12123/3803 https://www.sciencedirect.com/science/article/pii/S0042682215002457?via%3Dihub https://doi.org/10.1016/j.virol.2015.05.001 |
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