Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina
Peach (Prunus persica L.) plants with symptoms of yellowing, reddening, curling and leaf necrosis, premature defoliation and internode shortening were observed in production fields in Jujuy province (Argentina). A phytoplasma was detected by PCR using the universal primer pairs P1/P7 and R16F2n/R16R...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2018
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| Online Access: | http://hdl.handle.net/20.500.12123/3784 https://link.springer.com/article/10.1007/s10658-012-0109-z#citeas https://doi.org/10.1007/s10658-012-0109-z |
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| author | Fernandez, Franco Daniel Guzman, Fabiana Curzel, Viviana Bejarano, Noemí Conci, Luis Rogelio |
| author_browse | Bejarano, Noemí Conci, Luis Rogelio Curzel, Viviana Fernandez, Franco Daniel Guzman, Fabiana |
| author_facet | Fernandez, Franco Daniel Guzman, Fabiana Curzel, Viviana Bejarano, Noemí Conci, Luis Rogelio |
| author_sort | Fernandez, Franco Daniel |
| collection | INTA Digital |
| description | Peach (Prunus persica L.) plants with symptoms of yellowing, reddening, curling and leaf necrosis, premature defoliation and internode shortening were observed in production fields in Jujuy province (Argentina). A phytoplasma was detected by PCR using the universal primer pairs P1/P7 and R16F2n/R16R2 in all the symptomatic samples analysed. The RFLP profile of PCR products, amplified with R16F2n/R16R2 primers, shows that this phytoplasma, named Argentinean Peach Yellows (ArPY), belongs to subgroup 16Sr III-B. The phylogenetic analysis of the 1244 bp 16S rDNA cloned sequence, grouped the ArPY phytoplasma into the X-disease group with a closer relationship with CFSD, PssWB and ChTDIII phytoplasmas. This is the first report of a phytoplasma infecting peach trees in Argentina. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3784 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA37842022-04-20T16:09:44Z Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina Fernandez, Franco Daniel Guzman, Fabiana Curzel, Viviana Bejarano, Noemí Conci, Luis Rogelio Prunus Persica Fitoplasmas Durazno Clorosis Phytoplasmas Peaches Chlorosis PCR Molecular Characterization 16S rDNA Yellowing PCR-RFLP Argentina Peach (Prunus persica L.) plants with symptoms of yellowing, reddening, curling and leaf necrosis, premature defoliation and internode shortening were observed in production fields in Jujuy province (Argentina). A phytoplasma was detected by PCR using the universal primer pairs P1/P7 and R16F2n/R16R2 in all the symptomatic samples analysed. The RFLP profile of PCR products, amplified with R16F2n/R16R2 primers, shows that this phytoplasma, named Argentinean Peach Yellows (ArPY), belongs to subgroup 16Sr III-B. The phylogenetic analysis of the 1244 bp 16S rDNA cloned sequence, grouped the ArPY phytoplasma into the X-disease group with a closer relationship with CFSD, PssWB and ChTDIII phytoplasmas. This is the first report of a phytoplasma infecting peach trees in Argentina. Instituto de Patología Vegetal Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Guzman, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Curzel, Viviana. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias. Laboratorio de Diagnóstico de Enfermedades de las Plantas; Argentina Fil: Bejarano, Noemí. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias. Laboratorio de Diagnóstico de Enfermedades de las Plantas; Argentina Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Agronomía y Veterinaria; Argentina 2018-11-05T14:09:31Z 2018-11-05T14:09:31Z 2013-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3784 https://link.springer.com/article/10.1007/s10658-012-0109-z#citeas 0929-1873 1573-8469 (Online) https://doi.org/10.1007/s10658-012-0109-z eng info:eu-repo/semantics/restrictedAccess application/pdf Springer European journal of plant pathology 135 (4) : 627–631. (April 2013) |
| spellingShingle | Prunus Persica Fitoplasmas Durazno Clorosis Phytoplasmas Peaches Chlorosis PCR Molecular Characterization 16S rDNA Yellowing PCR-RFLP Argentina Fernandez, Franco Daniel Guzman, Fabiana Curzel, Viviana Bejarano, Noemí Conci, Luis Rogelio Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina |
| title | Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina |
| title_full | Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina |
| title_fullStr | Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina |
| title_full_unstemmed | Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina |
| title_short | Detection and molecular characterization of a phytoplasma affecting Prunus persica L. in Jujuy, Argentina |
| title_sort | detection and molecular characterization of a phytoplasma affecting prunus persica l in jujuy argentina |
| topic | Prunus Persica Fitoplasmas Durazno Clorosis Phytoplasmas Peaches Chlorosis PCR Molecular Characterization 16S rDNA Yellowing PCR-RFLP Argentina |
| url | http://hdl.handle.net/20.500.12123/3784 https://link.springer.com/article/10.1007/s10658-012-0109-z#citeas https://doi.org/10.1007/s10658-012-0109-z |
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