Mapping a region within the 1RS.1BL translocation in common wheat affecting grain yield and canopy water status
The translocation of the short arm of rye (Secale cereale L.) chromosome one (1RS) onto wheat (Triticum aestivum L.) chromosome 1B (1RS.1BL) is used in wheat breeding programs worldwide due to its positive effect on yield, particularly under abiotic stress. Unfortunately, this translocation is assoc...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2018
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| Online Access: | http://hdl.handle.net/20.500.12123/3781 https://link.springer.com/article/10.1007/s00122-014-2408-6#citeas https://doi.org/10.1007/s00122-014-2408-6 |
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| author | Howell, Tyson Hale, Iago Jankuloski, Ljupcho Bonafede, Marcos Gilbert, Matthew Dubcovsky, Jorge |
| author_browse | Bonafede, Marcos Dubcovsky, Jorge Gilbert, Matthew Hale, Iago Howell, Tyson Jankuloski, Ljupcho |
| author_facet | Howell, Tyson Hale, Iago Jankuloski, Ljupcho Bonafede, Marcos Gilbert, Matthew Dubcovsky, Jorge |
| author_sort | Howell, Tyson |
| collection | INTA Digital |
| description | The translocation of the short arm of rye (Secale cereale L.) chromosome one (1RS) onto wheat (Triticum aestivum L.) chromosome 1B (1RS.1BL) is used in wheat breeding programs worldwide due to its positive effect on yield, particularly under abiotic stress. Unfortunately, this translocation is associated with poor bread-making quality. To mitigate this problem, the 1RS arm was engineered by the removal and replacement of two interstitial rye segments with wheat chromatin: a distal segment to introduce the Glu-B3/Gli-B1 loci from wheat, and a proximal segment to remove the rye Sec-1 locus. We used this engineered 1RS chromosome (henceforth 1RSWW) to develop and evaluate two sets of 1RS/1RSWW near isogenic lines (NILs). Field trials showed that standard 1RS lines had significantly higher yield and better canopy water status than the 1RSWW NILs in both well-watered and water-stressed environments. We intercrossed the 1RS and 1RSWW lines and generated two additional NILs, one carrying the distal (1RSRW) and the other carrying the proximal (1RSWR) wheat segment. Lines not carrying the distal wheat region (1RS and 1RSWR) showed significant improvements in grain yield and canopy water status compared to NILs carrying the distal wheat segment (1RSWW and 1RSRW), indicating that the 1RS region replaced by the distal wheat segment carries the beneficial allele(s). NILs without the distal wheat segment also showed higher carbon isotope discrimination and increased stomatal conductance, suggesting that these plants had improved access to water. The 1RSWW, 1RSWR and 1RSRW NILs have been deposited in the National Small Grains Collection. |
| format | Artículo |
| id | INTA3781 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA37812018-11-05T13:26:58Z Mapping a region within the 1RS.1BL translocation in common wheat affecting grain yield and canopy water status Howell, Tyson Hale, Iago Jankuloski, Ljupcho Bonafede, Marcos Gilbert, Matthew Dubcovsky, Jorge Trigo Triticum Aestivum Conductancia Estomática Riego Rendimiento Centeno Cromosomas Wheat Irrigation Stomatal Conductance Yields Rye Chromosomes Irrigation Treatment Carbon Isotope Discrimination Canopy Spectral Reflectance Discriminación de Isótopos de Carbono The translocation of the short arm of rye (Secale cereale L.) chromosome one (1RS) onto wheat (Triticum aestivum L.) chromosome 1B (1RS.1BL) is used in wheat breeding programs worldwide due to its positive effect on yield, particularly under abiotic stress. Unfortunately, this translocation is associated with poor bread-making quality. To mitigate this problem, the 1RS arm was engineered by the removal and replacement of two interstitial rye segments with wheat chromatin: a distal segment to introduce the Glu-B3/Gli-B1 loci from wheat, and a proximal segment to remove the rye Sec-1 locus. We used this engineered 1RS chromosome (henceforth 1RSWW) to develop and evaluate two sets of 1RS/1RSWW near isogenic lines (NILs). Field trials showed that standard 1RS lines had significantly higher yield and better canopy water status than the 1RSWW NILs in both well-watered and water-stressed environments. We intercrossed the 1RS and 1RSWW lines and generated two additional NILs, one carrying the distal (1RSRW) and the other carrying the proximal (1RSWR) wheat segment. Lines not carrying the distal wheat region (1RS and 1RSWR) showed significant improvements in grain yield and canopy water status compared to NILs carrying the distal wheat segment (1RSWW and 1RSRW), indicating that the 1RS region replaced by the distal wheat segment carries the beneficial allele(s). NILs without the distal wheat segment also showed higher carbon isotope discrimination and increased stomatal conductance, suggesting that these plants had improved access to water. The 1RSWW, 1RSWR and 1RSRW NILs have been deposited in the National Small Grains Collection. Instituto de Recursos Biológicos Fil: Howell, Tyson. University of California. Department of Plant Sciences; Estados Unidos Fil: Hale, Iago. University of California. Department of Plant Sciences; Estados Unidos. University of New Hampshire. Department of Biological Sciences; Estados Unidos Fil: Jankuloski, Ljupcho. University of California. Department of Plant Sciences; Estados Unidos. Faculty of Agricultural Sciences and Food. Department of Genetics and Plant Breeding; Macedonia. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture. Plant Breeding and Genetics Section; Austria. Fil: Bonafede; Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina. University of California. Department of Plant Sciences; Estados Unidos Fil: Gilbert, Matthew. University of California. Department of Plant Sciences; Estados Unidos Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos 2018-11-05T13:21:56Z 2018-11-05T13:21:56Z 2014-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3781 https://link.springer.com/article/10.1007/s00122-014-2408-6#citeas 0040-5752 1432-2242 (Online) https://doi.org/10.1007/s00122-014-2408-6 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Theoretical and applied genetics 127 (12) : 2695–2709. (December 2014) |
| spellingShingle | Trigo Triticum Aestivum Conductancia Estomática Riego Rendimiento Centeno Cromosomas Wheat Irrigation Stomatal Conductance Yields Rye Chromosomes Irrigation Treatment Carbon Isotope Discrimination Canopy Spectral Reflectance Discriminación de Isótopos de Carbono Howell, Tyson Hale, Iago Jankuloski, Ljupcho Bonafede, Marcos Gilbert, Matthew Dubcovsky, Jorge Mapping a region within the 1RS.1BL translocation in common wheat affecting grain yield and canopy water status |
| title | Mapping a region within the 1RS.1BL translocation in common wheat affecting grain yield and canopy water status |
| title_full | Mapping a region within the 1RS.1BL translocation in common wheat affecting grain yield and canopy water status |
| title_fullStr | Mapping a region within the 1RS.1BL translocation in common wheat affecting grain yield and canopy water status |
| title_full_unstemmed | Mapping a region within the 1RS.1BL translocation in common wheat affecting grain yield and canopy water status |
| title_short | Mapping a region within the 1RS.1BL translocation in common wheat affecting grain yield and canopy water status |
| title_sort | mapping a region within the 1rs 1bl translocation in common wheat affecting grain yield and canopy water status |
| topic | Trigo Triticum Aestivum Conductancia Estomática Riego Rendimiento Centeno Cromosomas Wheat Irrigation Stomatal Conductance Yields Rye Chromosomes Irrigation Treatment Carbon Isotope Discrimination Canopy Spectral Reflectance Discriminación de Isótopos de Carbono |
| url | http://hdl.handle.net/20.500.12123/3781 https://link.springer.com/article/10.1007/s00122-014-2408-6#citeas https://doi.org/10.1007/s00122-014-2408-6 |
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