Sequencing of two Sunflower chlorotic mottle virus isolates obtained from different natural hosts shed light on its evolutionary history
Sunflower chlorotic mottle virus (SuCMoV), the most prevalent virus of sunflower in Argentina, was reported naturally infecting not only sunflower but also weeds. To understand SuCMoV evolution and improve the knowledge on its variability, the complete genomic sequences of two SuCMoV isolates collec...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2018
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| Online Access: | http://hdl.handle.net/20.500.12123/3778 |
| _version_ | 1855035201866956800 |
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| author | Bejerman, Nicolas Esteban Giolitti, Fabian De Breuil, Soledad Lenardon, Sergio Luis |
| author_browse | Bejerman, Nicolas Esteban De Breuil, Soledad Giolitti, Fabian Lenardon, Sergio Luis |
| author_facet | Bejerman, Nicolas Esteban Giolitti, Fabian De Breuil, Soledad Lenardon, Sergio Luis |
| author_sort | Bejerman, Nicolas Esteban |
| collection | INTA Digital |
| description | Sunflower chlorotic mottle virus (SuCMoV), the most prevalent virus of sunflower in Argentina, was reported naturally infecting not only sunflower but also weeds. To understand SuCMoV evolution and improve the knowledge on its variability, the complete genomic sequences of two SuCMoV isolates collected from Dipsacus fullonum (-dip) and Ibicella lutea (-ibi) were determined from three overlapping cDNA clones and subjected to phylogenetic and recombination analyses. SuCMoV-dip and -ibi genomes were 9,953-nucleotides (nt) long; their sequences contained an open reading frame of 9,561 nucleotides, which encoded a polyprotein of 3,187 amino acids flanked by a 5′-noncoding region (NCR) of 135 nt and a 3′-NCR of 257 nt. SuCMoV-dip and -ibi genome nucleotide sequences were 90.9 identical and displayed 90 and 94.6 % identity to that of SuCMoV-C, and 90.8 and 91.4 % identity to that of SuCMoV-CRS, respectively. P1 of SuCMoV-dip and -ibi was 3-nt longer than that of SuCMoV-CRS, but 12-nt shorter than that of SuCMoV-C. Two recombination events were detected in SuCMoV genome and the analysis of dN/dS ratio among SuCMoV complete sequences showed that the genomic regions are under different evolutionary constraints, suggesting that SuCMoV evolution would be conservative. Our findings provide evidence that mutation and recombination would have played important roles in the evolutionary history of SuCMoV. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3778 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA37782018-11-05T12:38:27Z Sequencing of two Sunflower chlorotic mottle virus isolates obtained from different natural hosts shed light on its evolutionary history Bejerman, Nicolas Esteban Giolitti, Fabian De Breuil, Soledad Lenardon, Sergio Luis Helianthus Annuus Potyvirus Genomas Potyviruses Genomes Girasol Secuenciación Análisis Filogenético Sunflower Sequencing Phylogenetic Analysis SuCMoV Sunflower chlorotic mottle virus (SuCMoV), the most prevalent virus of sunflower in Argentina, was reported naturally infecting not only sunflower but also weeds. To understand SuCMoV evolution and improve the knowledge on its variability, the complete genomic sequences of two SuCMoV isolates collected from Dipsacus fullonum (-dip) and Ibicella lutea (-ibi) were determined from three overlapping cDNA clones and subjected to phylogenetic and recombination analyses. SuCMoV-dip and -ibi genomes were 9,953-nucleotides (nt) long; their sequences contained an open reading frame of 9,561 nucleotides, which encoded a polyprotein of 3,187 amino acids flanked by a 5′-noncoding region (NCR) of 135 nt and a 3′-NCR of 257 nt. SuCMoV-dip and -ibi genome nucleotide sequences were 90.9 identical and displayed 90 and 94.6 % identity to that of SuCMoV-C, and 90.8 and 91.4 % identity to that of SuCMoV-CRS, respectively. P1 of SuCMoV-dip and -ibi was 3-nt longer than that of SuCMoV-CRS, but 12-nt shorter than that of SuCMoV-C. Two recombination events were detected in SuCMoV genome and the analysis of dN/dS ratio among SuCMoV complete sequences showed that the genomic regions are under different evolutionary constraints, suggesting that SuCMoV evolution would be conservative. Our findings provide evidence that mutation and recombination would have played important roles in the evolutionary history of SuCMoV. Instituto de Patología Vegetal Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2018-11-05T12:32:39Z 2018-11-05T12:32:39Z 2013-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3778 0920-8569 1572-994X (Online) eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Virus genes 46 (1) : 105–110. (February 2013) |
| spellingShingle | Helianthus Annuus Potyvirus Genomas Potyviruses Genomes Girasol Secuenciación Análisis Filogenético Sunflower Sequencing Phylogenetic Analysis SuCMoV Bejerman, Nicolas Esteban Giolitti, Fabian De Breuil, Soledad Lenardon, Sergio Luis Sequencing of two Sunflower chlorotic mottle virus isolates obtained from different natural hosts shed light on its evolutionary history |
| title | Sequencing of two Sunflower chlorotic mottle virus isolates obtained from different natural hosts shed light on its evolutionary history |
| title_full | Sequencing of two Sunflower chlorotic mottle virus isolates obtained from different natural hosts shed light on its evolutionary history |
| title_fullStr | Sequencing of two Sunflower chlorotic mottle virus isolates obtained from different natural hosts shed light on its evolutionary history |
| title_full_unstemmed | Sequencing of two Sunflower chlorotic mottle virus isolates obtained from different natural hosts shed light on its evolutionary history |
| title_short | Sequencing of two Sunflower chlorotic mottle virus isolates obtained from different natural hosts shed light on its evolutionary history |
| title_sort | sequencing of two sunflower chlorotic mottle virus isolates obtained from different natural hosts shed light on its evolutionary history |
| topic | Helianthus Annuus Potyvirus Genomas Potyviruses Genomes Girasol Secuenciación Análisis Filogenético Sunflower Sequencing Phylogenetic Analysis SuCMoV |
| url | http://hdl.handle.net/20.500.12123/3778 |
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