In vitro and in vivo characterization of a typical and a high pathogenic bovine viral diarrhea virus type ii strains
Non-cytopathic (ncp) type 2 bovine viral diarrhea virus (BVDV-2) is widely prevalent in Argentina causing high mortality rates in cattle herds. In this study, we characterized an Argentinean ncp BVDV-2 field isolate (98-124) compared to a high-virulence reference strain (NY-93), using in silico anal...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Frontiers Media
2018
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| Acceso en línea: | https://www.frontiersin.org/articles/10.3389/fvets.2018.00075/full http://hdl.handle.net/20.500.12123/3775 https://doi.org/10.3389/fvets.2018.00075 |
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| author | Malacari, Darío Amilcar Pecora, Andrea Perez Aguirreburialde, Maria Sol Cardoso, Nancy Patricia Odeon, Anselmo Carlos Capozzo, Alejandra Victoria |
| author_browse | Capozzo, Alejandra Victoria Cardoso, Nancy Patricia Malacari, Darío Amilcar Odeon, Anselmo Carlos Pecora, Andrea Perez Aguirreburialde, Maria Sol |
| author_facet | Malacari, Darío Amilcar Pecora, Andrea Perez Aguirreburialde, Maria Sol Cardoso, Nancy Patricia Odeon, Anselmo Carlos Capozzo, Alejandra Victoria |
| author_sort | Malacari, Darío Amilcar |
| collection | INTA Digital |
| description | Non-cytopathic (ncp) type 2 bovine viral diarrhea virus (BVDV-2) is widely prevalent in Argentina causing high mortality rates in cattle herds. In this study, we characterized an Argentinean ncp BVDV-2 field isolate (98-124) compared to a high-virulence reference strain (NY-93), using in silico analysis, in vitro assays, and in vivo infections of colostrum-deprived calves (CDC) to compare pathogenic characters and virulence. In vitro infection of bovine peripheral blood mononuclear cells (PBMC) with BVDV 98-124 induced necrosis shortly after infection while NY-93 strain increased the apoptotic rate in infected cells. Experimental infection of CDC (n = 4 each) with these strains caused an enteric syndrome. High pyrexia was detected in both groups. Viremia and shedding were more prolonged in the CDC infected with the NY-93 strain. In addition, NY-93 infection elicited a severe lymphopenia that lasted for 14 days, whereas 98-124 strain reduced the leukocyte counts for 5 days. All infected animals had a diminished lymphoproliferation activity in response to a mitogen. Neutralizing and anti-NS3 antibodies were detected 3 weeks after infection in all infected calves. Virulence was associated with a more severe clinical score, prolonged immune-suppression, and a greater window for transmission. Studies of apoptosis/necrosis performed after in vitro PBMC infection also revealed differences between both strains that might be correlated to the in vivo pathogenesis. Our results identified 98-124 as a low-virulence strain. |
| format | Artículo |
| id | INTA3775 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA37752019-01-23T14:07:30Z In vitro and in vivo characterization of a typical and a high pathogenic bovine viral diarrhea virus type ii strains Malacari, Darío Amilcar Pecora, Andrea Perez Aguirreburialde, Maria Sol Cardoso, Nancy Patricia Odeon, Anselmo Carlos Capozzo, Alejandra Victoria Bovine Diarrhoea Pestivirus Pathogenicity Virulence Experimental Infection Calves Pestivirus de la Diarrea Bovina Patogenicidad Virulencia Infección Experimental Ternero Argentina Non-cytopathic (ncp) type 2 bovine viral diarrhea virus (BVDV-2) is widely prevalent in Argentina causing high mortality rates in cattle herds. In this study, we characterized an Argentinean ncp BVDV-2 field isolate (98-124) compared to a high-virulence reference strain (NY-93), using in silico analysis, in vitro assays, and in vivo infections of colostrum-deprived calves (CDC) to compare pathogenic characters and virulence. In vitro infection of bovine peripheral blood mononuclear cells (PBMC) with BVDV 98-124 induced necrosis shortly after infection while NY-93 strain increased the apoptotic rate in infected cells. Experimental infection of CDC (n = 4 each) with these strains caused an enteric syndrome. High pyrexia was detected in both groups. Viremia and shedding were more prolonged in the CDC infected with the NY-93 strain. In addition, NY-93 infection elicited a severe lymphopenia that lasted for 14 days, whereas 98-124 strain reduced the leukocyte counts for 5 days. All infected animals had a diminished lymphoproliferation activity in response to a mitogen. Neutralizing and anti-NS3 antibodies were detected 3 weeks after infection in all infected calves. Virulence was associated with a more severe clinical score, prolonged immune-suppression, and a greater window for transmission. Studies of apoptosis/necrosis performed after in vitro PBMC infection also revealed differences between both strains that might be correlated to the in vivo pathogenesis. Our results identified 98-124 as a low-virulence strain. Instituto de Virología Fil: Malacari, Darío Amilcar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Pérez Aguirreburualde, María Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Cardoso, Nancy. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-11-05T11:02:20Z 2018-11-05T11:02:20Z 2018-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.frontiersin.org/articles/10.3389/fvets.2018.00075/full http://hdl.handle.net/20.500.12123/3775 2297-1769 https://doi.org/10.3389/fvets.2018.00075 eng info:eu-repograntAgreement/INTA/PNSA/1115053/AR./Biotecnologías reproductivas y desarrollo de metodologías de diagnóstico, control y prevención de las enfermedades infecciosas y parasitarias que afectan la concepción, gestación y período neonatal en especies de interés zootécnico. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Veterinary Science 5 : 75 (Abril 2018) |
| spellingShingle | Bovine Diarrhoea Pestivirus Pathogenicity Virulence Experimental Infection Calves Pestivirus de la Diarrea Bovina Patogenicidad Virulencia Infección Experimental Ternero Argentina Malacari, Darío Amilcar Pecora, Andrea Perez Aguirreburialde, Maria Sol Cardoso, Nancy Patricia Odeon, Anselmo Carlos Capozzo, Alejandra Victoria In vitro and in vivo characterization of a typical and a high pathogenic bovine viral diarrhea virus type ii strains |
| title | In vitro and in vivo characterization of a typical and a high pathogenic bovine viral diarrhea virus type ii strains |
| title_full | In vitro and in vivo characterization of a typical and a high pathogenic bovine viral diarrhea virus type ii strains |
| title_fullStr | In vitro and in vivo characterization of a typical and a high pathogenic bovine viral diarrhea virus type ii strains |
| title_full_unstemmed | In vitro and in vivo characterization of a typical and a high pathogenic bovine viral diarrhea virus type ii strains |
| title_short | In vitro and in vivo characterization of a typical and a high pathogenic bovine viral diarrhea virus type ii strains |
| title_sort | in vitro and in vivo characterization of a typical and a high pathogenic bovine viral diarrhea virus type ii strains |
| topic | Bovine Diarrhoea Pestivirus Pathogenicity Virulence Experimental Infection Calves Pestivirus de la Diarrea Bovina Patogenicidad Virulencia Infección Experimental Ternero Argentina |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2018.00075/full http://hdl.handle.net/20.500.12123/3775 https://doi.org/10.3389/fvets.2018.00075 |
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