Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry
The use of antimicrobial growth promoters (AGPs) in sub-therapeutic doses for long periods promotes the selection of resistant microorganisms and the subsequent risk of spreading this resistance to the human population and the environment. Global concern about antimicrobial resistance development an...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Frontiers Media
2018
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/3774 https://www.frontiersin.org/articles/10.3389/fmicb.2018.01679/full https://doi.org/10.3389/fmicb.2018.01679 |
| _version_ | 1855035201126662144 |
|---|---|
| author | Dominguez, Johanna Elizabeth Redondo, Leandro Martín Figueroa Espinosa, Roque A. Cejas, Daniela Gutkind, Gabriel Omar Chacana, Pablo Di Conza, José Alejandro Fernandez Miyakawa, Mariano Enrique |
| author_browse | Cejas, Daniela Chacana, Pablo Di Conza, José Alejandro Dominguez, Johanna Elizabeth Fernandez Miyakawa, Mariano Enrique Figueroa Espinosa, Roque A. Gutkind, Gabriel Omar Redondo, Leandro Martín |
| author_facet | Dominguez, Johanna Elizabeth Redondo, Leandro Martín Figueroa Espinosa, Roque A. Cejas, Daniela Gutkind, Gabriel Omar Chacana, Pablo Di Conza, José Alejandro Fernandez Miyakawa, Mariano Enrique |
| author_sort | Dominguez, Johanna Elizabeth |
| collection | INTA Digital |
| description | The use of antimicrobial growth promoters (AGPs) in sub-therapeutic doses for long periods promotes the selection of resistant microorganisms and the subsequent risk of spreading this resistance to the human population and the environment. Global concern about antimicrobial resistance development and transference of resistance genes from animal to human has been rising. The goal of our research was to evaluate the susceptibility pattern to different classes of antimicrobials of colistin-resistant Escherichia coli from poultry production systems that use AGPs, and characterize the resistance determinants associated to transferable platforms. E. coli strains (n = 41) were obtained from fecal samples collected from typical Argentine commercial broiler farms and susceptibility for 23 antimicrobials, relevant for human or veterinary medicine, was determined. Isolates were tested by PCR for the presence of mcr-1, extended spectrum β-lactamase encoding genes and plasmid-mediated quinolone resistance (PMQR) coding genes. Conjugation and susceptibility patterns of the transconjugant studies were performed. ERIC-PCR and REP-PCR analysis showed a high diversity of the isolates. Resistance to several antimicrobials was determined and all colistin-resistant isolates harbored the mcr-1 gene. CTX-M-2 cefotaximase was the main mechanism responsible for third generation cephalosporins resistance, and PMQR determinants were also identified. In addition, co-transference of the qnrB determinant on the mcr-1-positive transconjugants was corroborated, which suggests that these resistance genes are likely to be located in the same plasmid. In this work a wide range of antimicrobial resistance mechanisms were identified in E. coli strains isolated from the environment of healthy chickens highlighting the risk of antimicrobial abuse/misuse in animals under intensive production systems and its consequences for public health. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3774 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA37742021-12-10T14:03:18Z Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry Dominguez, Johanna Elizabeth Redondo, Leandro Martín Figueroa Espinosa, Roque A. Cejas, Daniela Gutkind, Gabriel Omar Chacana, Pablo Di Conza, José Alejandro Fernandez Miyakawa, Mariano Enrique Colistin Foodborne Diseases Drug Resistance Poultry Colistina Enfermedades Transmitidas por Alimentos Escherichia coli Resistencia a Medicamentos Aves de Corral Antimicrobial Resistance Resistencia Antimicrobiana The use of antimicrobial growth promoters (AGPs) in sub-therapeutic doses for long periods promotes the selection of resistant microorganisms and the subsequent risk of spreading this resistance to the human population and the environment. Global concern about antimicrobial resistance development and transference of resistance genes from animal to human has been rising. The goal of our research was to evaluate the susceptibility pattern to different classes of antimicrobials of colistin-resistant Escherichia coli from poultry production systems that use AGPs, and characterize the resistance determinants associated to transferable platforms. E. coli strains (n = 41) were obtained from fecal samples collected from typical Argentine commercial broiler farms and susceptibility for 23 antimicrobials, relevant for human or veterinary medicine, was determined. Isolates were tested by PCR for the presence of mcr-1, extended spectrum β-lactamase encoding genes and plasmid-mediated quinolone resistance (PMQR) coding genes. Conjugation and susceptibility patterns of the transconjugant studies were performed. ERIC-PCR and REP-PCR analysis showed a high diversity of the isolates. Resistance to several antimicrobials was determined and all colistin-resistant isolates harbored the mcr-1 gene. CTX-M-2 cefotaximase was the main mechanism responsible for third generation cephalosporins resistance, and PMQR determinants were also identified. In addition, co-transference of the qnrB determinant on the mcr-1-positive transconjugants was corroborated, which suggests that these resistance genes are likely to be located in the same plasmid. In this work a wide range of antimicrobial resistance mechanisms were identified in E. coli strains isolated from the environment of healthy chickens highlighting the risk of antimicrobial abuse/misuse in animals under intensive production systems and its consequences for public health. Instituto de Patobiología Fil: Dominguez, Johana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Redondo, Leandro Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Figueroa Espinosa, Roque A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cejas, Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gutkind, Gabriel O. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Di Conza, José A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-11-02T17:01:55Z 2018-11-02T17:01:55Z 2018-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3774 https://www.frontiersin.org/articles/10.3389/fmicb.2018.01679/full 1664-302X https://doi.org/10.3389/fmicb.2018.01679 eng info:eu-repograntAgreement/INTA/PNSA/1115056/AR./Enfermedades infecciosas de las aves. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Microbiology 25 (9): 1679 (Julio 2018) |
| spellingShingle | Colistin Foodborne Diseases Drug Resistance Poultry Colistina Enfermedades Transmitidas por Alimentos Escherichia coli Resistencia a Medicamentos Aves de Corral Antimicrobial Resistance Resistencia Antimicrobiana Dominguez, Johanna Elizabeth Redondo, Leandro Martín Figueroa Espinosa, Roque A. Cejas, Daniela Gutkind, Gabriel Omar Chacana, Pablo Di Conza, José Alejandro Fernandez Miyakawa, Mariano Enrique Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry |
| title | Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry |
| title_full | Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry |
| title_fullStr | Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry |
| title_full_unstemmed | Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry |
| title_short | Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry |
| title_sort | simultaneous carriage of mcr 1 and other antimicrobial resistance determinants in escherichia coli from poultry |
| topic | Colistin Foodborne Diseases Drug Resistance Poultry Colistina Enfermedades Transmitidas por Alimentos Escherichia coli Resistencia a Medicamentos Aves de Corral Antimicrobial Resistance Resistencia Antimicrobiana |
| url | http://hdl.handle.net/20.500.12123/3774 https://www.frontiersin.org/articles/10.3389/fmicb.2018.01679/full https://doi.org/10.3389/fmicb.2018.01679 |
| work_keys_str_mv | AT dominguezjohannaelizabeth simultaneouscarriageofmcr1andotherantimicrobialresistancedeterminantsinescherichiacolifrompoultry AT redondoleandromartin simultaneouscarriageofmcr1andotherantimicrobialresistancedeterminantsinescherichiacolifrompoultry AT figueroaespinosaroquea simultaneouscarriageofmcr1andotherantimicrobialresistancedeterminantsinescherichiacolifrompoultry AT cejasdaniela simultaneouscarriageofmcr1andotherantimicrobialresistancedeterminantsinescherichiacolifrompoultry AT gutkindgabrielomar simultaneouscarriageofmcr1andotherantimicrobialresistancedeterminantsinescherichiacolifrompoultry AT chacanapablo simultaneouscarriageofmcr1andotherantimicrobialresistancedeterminantsinescherichiacolifrompoultry AT diconzajosealejandro simultaneouscarriageofmcr1andotherantimicrobialresistancedeterminantsinescherichiacolifrompoultry AT fernandezmiyakawamarianoenrique simultaneouscarriageofmcr1andotherantimicrobialresistancedeterminantsinescherichiacolifrompoultry |