Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress
The establishment of a symbiotic interaction involves a signal exchange between the host legume (flavonoids) and the nitrogen-fixing rhizobia (nodulation factors (NFs)). Likewise, abiotic stress conditions, such as salinity and drought, strongly reduce the nodulation process, possibly affecting also...
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2018
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| Online Access: | https://link.springer.com/article/10.1007/s00374-013-0843-1 http://hdl.handle.net/20.500.12123/3765 https://doi.org/10.1007/s00374-013-0843-1 |
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| author | Muñoz, Nacira Belen Soria Díaz, Maria Eugenia Manyani, Hamid Contreras Sánchez Matamoros, Rocío Gil Serrano, Antonio Megías, Manuel Lascano, Hernan Ramiro |
| author_browse | Contreras Sánchez Matamoros, Rocío Gil Serrano, Antonio Lascano, Hernan Ramiro Manyani, Hamid Megías, Manuel Muñoz, Nacira Belen Soria Díaz, Maria Eugenia |
| author_facet | Muñoz, Nacira Belen Soria Díaz, Maria Eugenia Manyani, Hamid Contreras Sánchez Matamoros, Rocío Gil Serrano, Antonio Megías, Manuel Lascano, Hernan Ramiro |
| author_sort | Muñoz, Nacira Belen |
| collection | INTA Digital |
| description | The establishment of a symbiotic interaction involves a signal exchange between the host legume (flavonoids) and the nitrogen-fixing rhizobia (nodulation factors (NFs)). Likewise, abiotic stress conditions, such as salinity and drought, strongly reduce the nodulation process, possibly affecting also the signal exchange. In this work we characterized the structure and biological activity of NFs produced by Bradyrhizobium japonicum USDA 138 under control, salt, and osmotic stress conditions. This strain is the most widely used in Argentine soybean culture; under control conditions, it produces a mixture of four types of NFs (V(C16:0,MeFuc), V(C18:1,MeFuc), IV(C18:1), and V(C18:1,Ac,MeFuc)). Interestingly, under stress conditions, this strain produces new types of NFs, one common for both stress conditions (V(C16:1,MeFuc)) and another one only present under salt stress (IV(C18:1,MeFuc)). All mixtures of NFs, extracted from control, salt, and osmotic stress conditions, showed biological activity in soybean plants, such as root hair deformation, and the radical application of purified NFs induced systemic differences in dry matter accumulation. The inoculation of soybean with genistein-induced bacteria cultured under both control and stress conditions had a positive effect on the number of nodules formed and in some cases on dry matter accumulation. These responses are not related to changes in chlorophyll fluorescence or greenness index. |
| format | Artículo |
| id | INTA3765 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA37652018-11-02T12:18:43Z Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress Muñoz, Nacira Belen Soria Díaz, Maria Eugenia Manyani, Hamid Contreras Sánchez Matamoros, Rocío Gil Serrano, Antonio Megías, Manuel Lascano, Hernan Ramiro Soja Bradyrhizobium japonicum Estrés Osmótico Nodulación Estrés Abiótico Contenido de Materia Seca Soybeans Osmotic Stress Root Nodulation Abiotic Stress Dry Matter Content Estres Salino The establishment of a symbiotic interaction involves a signal exchange between the host legume (flavonoids) and the nitrogen-fixing rhizobia (nodulation factors (NFs)). Likewise, abiotic stress conditions, such as salinity and drought, strongly reduce the nodulation process, possibly affecting also the signal exchange. In this work we characterized the structure and biological activity of NFs produced by Bradyrhizobium japonicum USDA 138 under control, salt, and osmotic stress conditions. This strain is the most widely used in Argentine soybean culture; under control conditions, it produces a mixture of four types of NFs (V(C16:0,MeFuc), V(C18:1,MeFuc), IV(C18:1), and V(C18:1,Ac,MeFuc)). Interestingly, under stress conditions, this strain produces new types of NFs, one common for both stress conditions (V(C16:1,MeFuc)) and another one only present under salt stress (IV(C18:1,MeFuc)). All mixtures of NFs, extracted from control, salt, and osmotic stress conditions, showed biological activity in soybean plants, such as root hair deformation, and the radical application of purified NFs induced systemic differences in dry matter accumulation. The inoculation of soybean with genistein-induced bacteria cultured under both control and stress conditions had a positive effect on the number of nodules formed and in some cases on dry matter accumulation. These responses are not related to changes in chlorophyll fluorescence or greenness index. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Soria Díaz, Maria Eugenia. Universidad de Sevilla. Centro de Investigación, Tecnología e Innovación. Servicio de Espectrometría de Masas; España. Universidad de Sevilla. Departamento de Química Orgánica; España Fil: Manyani, Hamid. Universidad de Sevilla. Departamento de Microbiología y Parasitología; España Fil: Contreras Sánchez Matamoros, Rocío. Universidad de Sevilla. Departamento de Química Orgánica; España Fil: Gil Serrano, Antonio. Universidad de Sevilla. Departamento de Química Orgánica; España Fil: Megías, Manuel. Universidad de Sevilla. Departamento de Microbiología y Parasitología; España Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina 2018-11-02T12:16:07Z 2018-11-02T12:16:07Z 2014-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s00374-013-0843-1 http://hdl.handle.net/20.500.12123/3765 0178-2762 1432-0789 https://doi.org/10.1007/s00374-013-0843-1 eng info:eu-repo/semantics/restrictedAccess application/pdf Biology and Fertility of Soils 50 (2) : 207–215 (February 2014) |
| spellingShingle | Soja Bradyrhizobium japonicum Estrés Osmótico Nodulación Estrés Abiótico Contenido de Materia Seca Soybeans Osmotic Stress Root Nodulation Abiotic Stress Dry Matter Content Estres Salino Muñoz, Nacira Belen Soria Díaz, Maria Eugenia Manyani, Hamid Contreras Sánchez Matamoros, Rocío Gil Serrano, Antonio Megías, Manuel Lascano, Hernan Ramiro Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress |
| title | Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress |
| title_full | Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress |
| title_fullStr | Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress |
| title_full_unstemmed | Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress |
| title_short | Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress |
| title_sort | structure and biological activities of lipochitooligosaccharide nodulation signals produced by bradyrhizobium japonicum usda 138 under saline and osmotic stress |
| topic | Soja Bradyrhizobium japonicum Estrés Osmótico Nodulación Estrés Abiótico Contenido de Materia Seca Soybeans Osmotic Stress Root Nodulation Abiotic Stress Dry Matter Content Estres Salino |
| url | https://link.springer.com/article/10.1007/s00374-013-0843-1 http://hdl.handle.net/20.500.12123/3765 https://doi.org/10.1007/s00374-013-0843-1 |
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