Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina
Sunflower (Helianthus annuus L.) plants showing capitulum with virescence, phyllody and flower malformation, shortened internodes and abnormal branches were found in a field in Pedro Luro (Buenos Aires province, Argentina). Pleomorphic bodies resembling phytoplasmas were observed in sieve tube eleme...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/3763 https://link.springer.com/article/10.1007%2Fs10658-013-0352-y#citeas https://doi.org/10.1007/s10658-013-0352-y |
| _version_ | 1855483298297413632 |
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| author | Guzmán, Fabiana Giolitti, Fabian Fernandez, Franco Daniel Nome Docampo, Claudia Lenardon, Sergio Luis Conci, Luis Rogelio |
| author_browse | Conci, Luis Rogelio Fernandez, Franco Daniel Giolitti, Fabian Guzmán, Fabiana Lenardon, Sergio Luis Nome Docampo, Claudia |
| author_facet | Guzmán, Fabiana Giolitti, Fabian Fernandez, Franco Daniel Nome Docampo, Claudia Lenardon, Sergio Luis Conci, Luis Rogelio |
| author_sort | Guzmán, Fabiana |
| collection | INTA Digital |
| description | Sunflower (Helianthus annuus L.) plants showing capitulum with virescence, phyllody and flower malformation, shortened internodes and abnormal branches were found in a field in Pedro Luro (Buenos Aires province, Argentina). Pleomorphic bodies resembling phytoplasmas were observed in sieve tube elements of symptomatic plants but not in healthy ones. DNA from all symptomatic sunflower plants analysed yielded, in direct PCR with phytoplasma universal primers P1/P7 and R16F2n/R2, fragments of expected size 1.8 kb and 1.2 kb, respectively. The phytoplasma associated with the disease, was named Sunflower Phyllody (SunPhy). Real and putative RFLP of the 16S rDNA showed the affiliation of SunPhy to 16SrIII (X-disease group), subgroup J. The 16S rDNA sequence from SunPhy showed the highest identity (99 %) with 16SrIII members and the phylogenetic tree confirmed a closer relationship to subgroup J of the 16SIII ribosomal group. This is the first report of a phytoplasma related to the 16SrIII group affecting sunflower. |
| format | Artículo |
| id | INTA3763 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA37632022-04-20T16:11:57Z Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina Guzmán, Fabiana Giolitti, Fabian Fernandez, Franco Daniel Nome Docampo, Claudia Lenardon, Sergio Luis Conci, Luis Rogelio Phytoplasmas Fitoplasmas Helianthus Annuus Argentina Girasol RFLP-PCR Secuenciación Sunflower Sequencing 16SrDNA X-disease Sunflower (Helianthus annuus L.) plants showing capitulum with virescence, phyllody and flower malformation, shortened internodes and abnormal branches were found in a field in Pedro Luro (Buenos Aires province, Argentina). Pleomorphic bodies resembling phytoplasmas were observed in sieve tube elements of symptomatic plants but not in healthy ones. DNA from all symptomatic sunflower plants analysed yielded, in direct PCR with phytoplasma universal primers P1/P7 and R16F2n/R2, fragments of expected size 1.8 kb and 1.2 kb, respectively. The phytoplasma associated with the disease, was named Sunflower Phyllody (SunPhy). Real and putative RFLP of the 16S rDNA showed the affiliation of SunPhy to 16SrIII (X-disease group), subgroup J. The 16S rDNA sequence from SunPhy showed the highest identity (99 %) with 16SrIII members and the phylogenetic tree confirmed a closer relationship to subgroup J of the 16SIII ribosomal group. This is the first report of a phytoplasma related to the 16SrIII group affecting sunflower. Instituto de Patología Vegetal Fil: Guzmán, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Agronomía y Veterinaria; Argentina 2018-11-01T17:27:20Z 2018-11-01T17:27:20Z 2014-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3763 https://link.springer.com/article/10.1007%2Fs10658-013-0352-y#citeas 0929-1873 1573-8469 (Online) https://doi.org/10.1007/s10658-013-0352-y eng info:eu-repo/semantics/restrictedAccess application/pdf Springer European journal of plant pathology 138 (4) : 679–683. (April 2014) |
| spellingShingle | Phytoplasmas Fitoplasmas Helianthus Annuus Argentina Girasol RFLP-PCR Secuenciación Sunflower Sequencing 16SrDNA X-disease Guzmán, Fabiana Giolitti, Fabian Fernandez, Franco Daniel Nome Docampo, Claudia Lenardon, Sergio Luis Conci, Luis Rogelio Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina |
| title | Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina |
| title_full | Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina |
| title_fullStr | Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina |
| title_full_unstemmed | Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina |
| title_short | Identification and molecular characterization of a phytoplasma associated with sunflower in Argentina |
| title_sort | identification and molecular characterization of a phytoplasma associated with sunflower in argentina |
| topic | Phytoplasmas Fitoplasmas Helianthus Annuus Argentina Girasol RFLP-PCR Secuenciación Sunflower Sequencing 16SrDNA X-disease |
| url | http://hdl.handle.net/20.500.12123/3763 https://link.springer.com/article/10.1007%2Fs10658-013-0352-y#citeas https://doi.org/10.1007/s10658-013-0352-y |
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