Cysteine proteinase C1A paralog profiles correspond with phylogenetic lineages of pathogenic piroplasmids

Piroplasmid parasites comprising of Babesia, Theileria, and Cytauxzoon are transmitted by ticks to farm and pet animals and have a significant impact on livestock industries and animal health in tropical and subtropical regions worldwide. In addition, diverse Babesia spp. infect humans as opportunis...

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Autores principales: Ascencio, Mariano, Florin-Christensen, Mónica, Mamoun, Choukri B., Weir, William, Shiels, Brian, Schnittger, Leonhard
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.mdpi.com/2306-7381/5/2/41
http://hdl.handle.net/20.500.12123/3751
https://doi.org/10.3390/vetsci5020041
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author Ascencio, Mariano
Florin-Christensen, Mónica
Mamoun, Choukri B.
Weir, William
Shiels, Brian
Schnittger, Leonhard
author_browse Ascencio, Mariano
Florin-Christensen, Mónica
Mamoun, Choukri B.
Schnittger, Leonhard
Shiels, Brian
Weir, William
author_facet Ascencio, Mariano
Florin-Christensen, Mónica
Mamoun, Choukri B.
Weir, William
Shiels, Brian
Schnittger, Leonhard
author_sort Ascencio, Mariano
collection INTA Digital
description Piroplasmid parasites comprising of Babesia, Theileria, and Cytauxzoon are transmitted by ticks to farm and pet animals and have a significant impact on livestock industries and animal health in tropical and subtropical regions worldwide. In addition, diverse Babesia spp. infect humans as opportunistic hosts. Molecular phylogeny has demonstrated at least six piroplasmid lineages exemplified by B. microti, B. duncani, C. felis, T. equi, Theileria sensu stricto (T. annulata, T. parva, and T. orientalis) and Babesia sensu stricto (B. bovis, B. bigemina, and B. ovis). C1A cysteine-proteinases (C1A-Cp) are papain-like enzymes implicated in pathogenic and vital steps of the parasite life cycle such as nutrition and host cell egress. An expansion of C1A-Cp of T. annulata and T. parva with respect to B. bovis and B. ovis was previously described. In the present work, C1A-Cp paralogs were identified in available genomes of species pertaining to each piroplasmid lineage. Phylogenetic analysis revealed eight C1A-Cp groups. The profile of C1A-Cp paralogs across these groups corroborates and defines the existence of six piroplasmid lineages. C. felis, T. equi and Theileria s.s. each showed characteristic expansions into extensive families of C1A-Cp paralogs in two of the eight groups. Underlying gene duplications have occurred as independent unique evolutionary events that allow distinguishing these three piroplasmid lineages. We hypothesize that C1A-Cp paralog families may be associated with the advent of the schizont stage. Differences in the invertebrate tick host specificity and/or mode of transmission in piroplasmid lineages might also be associated with the observed C1A-Cp paralog profiles.
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spelling INTA37512021-11-15T16:35:38Z Cysteine proteinase C1A paralog profiles correspond with phylogenetic lineages of pathogenic piroplasmids Ascencio, Mariano Florin-Christensen, Mónica Mamoun, Choukri B. Weir, William Shiels, Brian Schnittger, Leonhard Babesia Cisteína Cytauxzoon Theileria Genómica Filogenia Piroplasmea Taxonomía Metastigmata Enfermedades transmitidas por garrapatas Cysteine Genomics Phylogeny Taxonomy Tickborne diseases Piroplasmid parasites comprising of Babesia, Theileria, and Cytauxzoon are transmitted by ticks to farm and pet animals and have a significant impact on livestock industries and animal health in tropical and subtropical regions worldwide. In addition, diverse Babesia spp. infect humans as opportunistic hosts. Molecular phylogeny has demonstrated at least six piroplasmid lineages exemplified by B. microti, B. duncani, C. felis, T. equi, Theileria sensu stricto (T. annulata, T. parva, and T. orientalis) and Babesia sensu stricto (B. bovis, B. bigemina, and B. ovis). C1A cysteine-proteinases (C1A-Cp) are papain-like enzymes implicated in pathogenic and vital steps of the parasite life cycle such as nutrition and host cell egress. An expansion of C1A-Cp of T. annulata and T. parva with respect to B. bovis and B. ovis was previously described. In the present work, C1A-Cp paralogs were identified in available genomes of species pertaining to each piroplasmid lineage. Phylogenetic analysis revealed eight C1A-Cp groups. The profile of C1A-Cp paralogs across these groups corroborates and defines the existence of six piroplasmid lineages. C. felis, T. equi and Theileria s.s. each showed characteristic expansions into extensive families of C1A-Cp paralogs in two of the eight groups. Underlying gene duplications have occurred as independent unique evolutionary events that allow distinguishing these three piroplasmid lineages. We hypothesize that C1A-Cp paralog families may be associated with the advent of the schizont stage. Differences in the invertebrate tick host specificity and/or mode of transmission in piroplasmid lineages might also be associated with the observed C1A-Cp paralog profiles. Instituto de Patobiología Fil: Ascencio, Mariano E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mamoun, Choukri B. Yale School of Medicine. Department of Internal Medicine; Estados Unidos Fil: Weir, William. University of Glasgow. Institute of Biodiversity, Animal Health and Comparative Medicine; Reino Unido Fil: Shiels, Brian. University of Glasgow. Institute of Biodiversity, Animal Health and Comparative Medicine; Reino Unido Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-10-31T18:39:33Z 2018-10-31T18:39:33Z 2018-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.mdpi.com/2306-7381/5/2/41 http://hdl.handle.net/20.500.12123/3751 2306-7381 https://doi.org/10.3390/vetsci5020041 eng info:eu-repograntAgreement/INTA/PNBIO/1131034/AR./Inmunología molecular y genómica funcional aplicadas a interacciones patógeno hospedador de interés pecuario. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Veterinary Sciences 5 (2) : 41 (Abril 2018)
spellingShingle Babesia
Cisteína
Cytauxzoon
Theileria
Genómica
Filogenia
Piroplasmea
Taxonomía
Metastigmata
Enfermedades transmitidas por garrapatas
Cysteine
Genomics
Phylogeny
Taxonomy
Tickborne diseases
Ascencio, Mariano
Florin-Christensen, Mónica
Mamoun, Choukri B.
Weir, William
Shiels, Brian
Schnittger, Leonhard
Cysteine proteinase C1A paralog profiles correspond with phylogenetic lineages of pathogenic piroplasmids
title Cysteine proteinase C1A paralog profiles correspond with phylogenetic lineages of pathogenic piroplasmids
title_full Cysteine proteinase C1A paralog profiles correspond with phylogenetic lineages of pathogenic piroplasmids
title_fullStr Cysteine proteinase C1A paralog profiles correspond with phylogenetic lineages of pathogenic piroplasmids
title_full_unstemmed Cysteine proteinase C1A paralog profiles correspond with phylogenetic lineages of pathogenic piroplasmids
title_short Cysteine proteinase C1A paralog profiles correspond with phylogenetic lineages of pathogenic piroplasmids
title_sort cysteine proteinase c1a paralog profiles correspond with phylogenetic lineages of pathogenic piroplasmids
topic Babesia
Cisteína
Cytauxzoon
Theileria
Genómica
Filogenia
Piroplasmea
Taxonomía
Metastigmata
Enfermedades transmitidas por garrapatas
Cysteine
Genomics
Phylogeny
Taxonomy
Tickborne diseases
url https://www.mdpi.com/2306-7381/5/2/41
http://hdl.handle.net/20.500.12123/3751
https://doi.org/10.3390/vetsci5020041
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