Complete nucleotide sequence of Alfalfa mosaic virus isolated from alfalfa (Medicago sativa L.) in Argentina
The complete nucleotide sequence of an Alfalfa mosaic virus (AMV) isolate infecting alfalfa (Medicago sativa L.) in Argentina, AMV-Arg, was determined. The virus genome has the typical organization described for AMV, and comprises 3,643, 2,593, and 2,038 nucleotides for RNA1, 2 and 3, respectively....
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/3750 https://doi.org/10.1007/s11262-014-1045-0 |
| _version_ | 1855483295983206400 |
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| author | Trucco, Veronica Milagros De Breuil, Soledad Bejerman, Nicolas Esteban Lenardon, Sergio Luis Giolitti, Fabian |
| author_browse | Bejerman, Nicolas Esteban De Breuil, Soledad Giolitti, Fabian Lenardon, Sergio Luis Trucco, Veronica Milagros |
| author_facet | Trucco, Veronica Milagros De Breuil, Soledad Bejerman, Nicolas Esteban Lenardon, Sergio Luis Giolitti, Fabian |
| author_sort | Trucco, Veronica Milagros |
| collection | INTA Digital |
| description | The complete nucleotide sequence of an Alfalfa mosaic virus (AMV) isolate infecting alfalfa (Medicago sativa L.) in Argentina, AMV-Arg, was determined. The virus genome has the typical organization described for AMV, and comprises 3,643, 2,593, and 2,038 nucleotides for RNA1, 2 and 3, respectively. The whole genome sequence and each encoding region were compared with those of other four isolates that have been completely sequenced from China, Italy, Spain and USA. The nucleotide identity percentages ranged from 95.9 to 99.1 % for the three RNAs and from 93.7 to 99 % for the protein 1 (P1), protein 2 (P2), movement protein and coat protein (CP) encoding regions, whereas the amino acid identity percentages of these proteins ranged from 93.4 to 99.5 %, the lowest value corresponding to P2. CP sequences of AMV-Arg were compared with those of other 25 available isolates, and the phylogenetic analysis based on the CP gene was carried out. The highest percentage of nucleotide sequence identity of the CP gene was 98.3 % with a Chinese isolate and 98.6 % at the amino acid level with four isolates, two from Italy, one from Brazil and the remaining one from China. The phylogenetic analysis showed that AMV-Arg is closely related to subgroup I of AMV isolates. To our knowledge, this is the first report of a complete nucleotide sequence of AMV from South America and the first worldwide report of complete nucleotide sequence of AMV isolated from alfalfa as natural host. |
| format | Artículo |
| id | INTA3750 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA37502018-10-31T18:13:53Z Complete nucleotide sequence of Alfalfa mosaic virus isolated from alfalfa (Medicago sativa L.) in Argentina Trucco, Veronica Milagros De Breuil, Soledad Bejerman, Nicolas Esteban Lenardon, Sergio Luis Giolitti, Fabian Medicago Sativa Secuencia Nucleotídica Genomas Nucleotide Sequence Genomes Alfalfa Argentina Virus del Mosaico de la Alfalfa Relaciones Filogenéticas Alfalfa Mosaic Virus Phylogenetic Relationships The complete nucleotide sequence of an Alfalfa mosaic virus (AMV) isolate infecting alfalfa (Medicago sativa L.) in Argentina, AMV-Arg, was determined. The virus genome has the typical organization described for AMV, and comprises 3,643, 2,593, and 2,038 nucleotides for RNA1, 2 and 3, respectively. The whole genome sequence and each encoding region were compared with those of other four isolates that have been completely sequenced from China, Italy, Spain and USA. The nucleotide identity percentages ranged from 95.9 to 99.1 % for the three RNAs and from 93.7 to 99 % for the protein 1 (P1), protein 2 (P2), movement protein and coat protein (CP) encoding regions, whereas the amino acid identity percentages of these proteins ranged from 93.4 to 99.5 %, the lowest value corresponding to P2. CP sequences of AMV-Arg were compared with those of other 25 available isolates, and the phylogenetic analysis based on the CP gene was carried out. The highest percentage of nucleotide sequence identity of the CP gene was 98.3 % with a Chinese isolate and 98.6 % at the amino acid level with four isolates, two from Italy, one from Brazil and the remaining one from China. The phylogenetic analysis showed that AMV-Arg is closely related to subgroup I of AMV isolates. To our knowledge, this is the first report of a complete nucleotide sequence of AMV from South America and the first worldwide report of complete nucleotide sequence of AMV isolated from alfalfa as natural host. Instituto de Patología Vegetal Fil: Trucco, Veronica Milagros: Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2018-10-31T18:07:56Z 2018-10-31T18:07:56Z 2014-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3750 0920-8569 1572-994X (Online) https://doi.org/10.1007/s11262-014-1045-0 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) Springer Virus genes 48 (3) : 562–565. (June 2014) |
| spellingShingle | Medicago Sativa Secuencia Nucleotídica Genomas Nucleotide Sequence Genomes Alfalfa Argentina Virus del Mosaico de la Alfalfa Relaciones Filogenéticas Alfalfa Mosaic Virus Phylogenetic Relationships Trucco, Veronica Milagros De Breuil, Soledad Bejerman, Nicolas Esteban Lenardon, Sergio Luis Giolitti, Fabian Complete nucleotide sequence of Alfalfa mosaic virus isolated from alfalfa (Medicago sativa L.) in Argentina |
| title | Complete nucleotide sequence of Alfalfa mosaic virus isolated from alfalfa (Medicago sativa L.) in Argentina |
| title_full | Complete nucleotide sequence of Alfalfa mosaic virus isolated from alfalfa (Medicago sativa L.) in Argentina |
| title_fullStr | Complete nucleotide sequence of Alfalfa mosaic virus isolated from alfalfa (Medicago sativa L.) in Argentina |
| title_full_unstemmed | Complete nucleotide sequence of Alfalfa mosaic virus isolated from alfalfa (Medicago sativa L.) in Argentina |
| title_short | Complete nucleotide sequence of Alfalfa mosaic virus isolated from alfalfa (Medicago sativa L.) in Argentina |
| title_sort | complete nucleotide sequence of alfalfa mosaic virus isolated from alfalfa medicago sativa l in argentina |
| topic | Medicago Sativa Secuencia Nucleotídica Genomas Nucleotide Sequence Genomes Alfalfa Argentina Virus del Mosaico de la Alfalfa Relaciones Filogenéticas Alfalfa Mosaic Virus Phylogenetic Relationships |
| url | http://hdl.handle.net/20.500.12123/3750 https://doi.org/10.1007/s11262-014-1045-0 |
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