Diversity and stress tolerance in rhizobia from Parque Chaqueño region of Argentina nodulating Prosopis alba

The aim of this work was to investigate the genetic diversity, symbiotic effectiveness, drought tolerance, and indole acetic acid production of indigenous rhizobial populations in the Parque Chaqueño of Argentina able to nodulate Prosopis alba, the dominant forest tree of this region. The population...

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Autores principales: Chávez Díaz, Lucía, Gonzalez, Pablo, Rubio, Esteban Julian, Melchiorre, Mariana
Formato: Artículo
Lenguaje:Inglés
Publicado: Springer 2018
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/3746
https://link.springer.com/article/10.1007/s00374-013-0814-6#citeas
https://doi.org/10.1007/s00374-013-0814-6
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author Chávez Díaz, Lucía
Gonzalez, Pablo
Rubio, Esteban Julian
Melchiorre, Mariana
author_browse Chávez Díaz, Lucía
Gonzalez, Pablo
Melchiorre, Mariana
Rubio, Esteban Julian
author_facet Chávez Díaz, Lucía
Gonzalez, Pablo
Rubio, Esteban Julian
Melchiorre, Mariana
author_sort Chávez Díaz, Lucía
collection INTA Digital
description The aim of this work was to investigate the genetic diversity, symbiotic effectiveness, drought tolerance, and indole acetic acid production of indigenous rhizobial populations in the Parque Chaqueño of Argentina able to nodulate Prosopis alba, the dominant forest tree of this region. The populations were sampled at five locations from the Arid, Semi-arid, and Humid Chaco in the Parque Chaqueño region. A set of rhizobial strains able to nodulate P. alba was obtained and selected based on their molecular diversity. Data obtained by BOX-PCR indicated that the highest molecular variability was observed in rhizobial isolates from Semi-arid Chaco. High level of indolic compound production and tolerance to osmotic treatment were significantly (p ≤ 0.05) correlated with water restrictions of the environments where the strains belonged. A small set of rhizobial strains that stimulate P. alba growth was selected from a large group of strains. The strains were identified by 16S rDNA sequencing as belonging to the genera Mesorhizobium, Bradyrhizobium, and Ensifer. To our knowledge, this is the first report of P. alba nodulation by strains other than Mesorhizobium chacoense, which was already described for the Parque Chaqueño.
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spelling INTA37462018-10-30T17:52:10Z Diversity and stress tolerance in rhizobia from Parque Chaqueño region of Argentina nodulating Prosopis alba Chávez Díaz, Lucía Gonzalez, Pablo Rubio, Esteban Julian Melchiorre, Mariana Prosopis Alba Simbiosis Rhizobiaceae Variación Genética Symbiosis Genetic Variation Drought Tolerance Tolerancia a la Sequía Región Chaqueña The aim of this work was to investigate the genetic diversity, symbiotic effectiveness, drought tolerance, and indole acetic acid production of indigenous rhizobial populations in the Parque Chaqueño of Argentina able to nodulate Prosopis alba, the dominant forest tree of this region. The populations were sampled at five locations from the Arid, Semi-arid, and Humid Chaco in the Parque Chaqueño region. A set of rhizobial strains able to nodulate P. alba was obtained and selected based on their molecular diversity. Data obtained by BOX-PCR indicated that the highest molecular variability was observed in rhizobial isolates from Semi-arid Chaco. High level of indolic compound production and tolerance to osmotic treatment were significantly (p ≤ 0.05) correlated with water restrictions of the environments where the strains belonged. A small set of rhizobial strains that stimulate P. alba growth was selected from a large group of strains. The strains were identified by 16S rDNA sequencing as belonging to the genera Mesorhizobium, Bradyrhizobium, and Ensifer. To our knowledge, this is the first report of P. alba nodulation by strains other than Mesorhizobium chacoense, which was already described for the Parque Chaqueño. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Chávez Díaz, Lucía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Gonzalez, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Rubio, Esteban Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Zoología y Microbiología Agrícola; Argentina Fil: Melchiorre, Mariana Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Zoología y Microbiología Agrícola; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; Argentina 2018-10-30T17:45:47Z 2018-10-30T17:45:47Z 2013-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3746 https://link.springer.com/article/10.1007/s00374-013-0814-6#citeas 0178-2762 1432-0789 (Online) https://doi.org/10.1007/s00374-013-0814-6 eng info:eu-repo/semantics/restrictedAccess application/pdf Chaco (province) Springer Biology and fertility of soils 49 (8) : 1153–1165. (November 2013)
spellingShingle Prosopis Alba
Simbiosis
Rhizobiaceae
Variación Genética
Symbiosis
Genetic Variation
Drought Tolerance
Tolerancia a la Sequía
Región Chaqueña
Chávez Díaz, Lucía
Gonzalez, Pablo
Rubio, Esteban Julian
Melchiorre, Mariana
Diversity and stress tolerance in rhizobia from Parque Chaqueño region of Argentina nodulating Prosopis alba
title Diversity and stress tolerance in rhizobia from Parque Chaqueño region of Argentina nodulating Prosopis alba
title_full Diversity and stress tolerance in rhizobia from Parque Chaqueño region of Argentina nodulating Prosopis alba
title_fullStr Diversity and stress tolerance in rhizobia from Parque Chaqueño region of Argentina nodulating Prosopis alba
title_full_unstemmed Diversity and stress tolerance in rhizobia from Parque Chaqueño region of Argentina nodulating Prosopis alba
title_short Diversity and stress tolerance in rhizobia from Parque Chaqueño region of Argentina nodulating Prosopis alba
title_sort diversity and stress tolerance in rhizobia from parque chaqueno region of argentina nodulating prosopis alba
topic Prosopis Alba
Simbiosis
Rhizobiaceae
Variación Genética
Symbiosis
Genetic Variation
Drought Tolerance
Tolerancia a la Sequía
Región Chaqueña
url http://hdl.handle.net/20.500.12123/3746
https://link.springer.com/article/10.1007/s00374-013-0814-6#citeas
https://doi.org/10.1007/s00374-013-0814-6
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