Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic micro particles as bio-affinity platform
In this article, we report the first integrated microfluidic immunosensor coupled to a screen-printed carbon electrode (SPCE) applied to determination of clenbuterol (CLB) in bovine hair samples. CLB is a member of the β2-agonist drugs which is used in animal production and is banned in Argentine an...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2018
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S0956566312005441 http://hdl.handle.net/20.500.12123/3739 https://doi.org/10.1016/j.bios.2012.08.020 |
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| author | Regiart, Daniel Matias Gaston Fernández Baldo, Martín Alejandro Spotorno, Viviana Graciela Bertolino, Franco Adrián Raba, Julio |
| author_browse | Bertolino, Franco Adrián Fernández Baldo, Martín Alejandro Raba, Julio Regiart, Daniel Matias Gaston Spotorno, Viviana Graciela |
| author_facet | Regiart, Daniel Matias Gaston Fernández Baldo, Martín Alejandro Spotorno, Viviana Graciela Bertolino, Franco Adrián Raba, Julio |
| author_sort | Regiart, Daniel Matias Gaston |
| collection | INTA Digital |
| description | In this article, we report the first integrated microfluidic immunosensor coupled to a screen-printed carbon electrode (SPCE) applied to determination of clenbuterol (CLB) in bovine hair samples. CLB is a member of the β2-agonist drugs which is used in animal production and is banned in Argentine and the European Union. It represents a potential risk and has to be carefully monitored to avoid the illegal use of high amounts of this compound that could result in human food poisoning. In order to perform the CLB detection, the SPCE was modified by gold nanoparticles (AuNPs) electrodeposition. Quantitative determination of CLB was carried out using a competitive indirect immunoassay, method based on the use of anti-CLB antibodies immobilized on magnetic micro particles. The CLB present in bovine hair samples competes immunologically with alkaline phosphatase (AP) enzyme-labeled CLB conjugate for the anti-CLB specific antibodies. Later, p-aminophenyl phosphate was converted to p-aminophenol by AP, and the electroactive product was quantified on AuNPs/SPCE at +0.1 V. The limit of detection for electrochemical method was 0.008 ng mL−1 and the intra- and inter-assay coefficients of variation were below 6%. This being a veterinary control tool very useful for rapid, sensitive and selective detection of CLB in an “in vitro” technique. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3739 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA37392018-10-30T12:31:28Z Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic micro particles as bio-affinity platform Regiart, Daniel Matias Gaston Fernández Baldo, Martín Alejandro Spotorno, Viviana Graciela Bertolino, Franco Adrián Raba, Julio Clenbuterol Ganado Bovino Nanotecnología Inocuidad Alimentaria Medicamentos Neurotrópicos Cattle Nanotechnology Food Safety Neurotropic Drugs Inmunosensores In this article, we report the first integrated microfluidic immunosensor coupled to a screen-printed carbon electrode (SPCE) applied to determination of clenbuterol (CLB) in bovine hair samples. CLB is a member of the β2-agonist drugs which is used in animal production and is banned in Argentine and the European Union. It represents a potential risk and has to be carefully monitored to avoid the illegal use of high amounts of this compound that could result in human food poisoning. In order to perform the CLB detection, the SPCE was modified by gold nanoparticles (AuNPs) electrodeposition. Quantitative determination of CLB was carried out using a competitive indirect immunoassay, method based on the use of anti-CLB antibodies immobilized on magnetic micro particles. The CLB present in bovine hair samples competes immunologically with alkaline phosphatase (AP) enzyme-labeled CLB conjugate for the anti-CLB specific antibodies. Later, p-aminophenyl phosphate was converted to p-aminophenol by AP, and the electroactive product was quantified on AuNPs/SPCE at +0.1 V. The limit of detection for electrochemical method was 0.008 ng mL−1 and the intra- and inter-assay coefficients of variation were below 6%. This being a veterinary control tool very useful for rapid, sensitive and selective detection of CLB in an “in vitro” technique. Instituto de Recursos Biológicos Fil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina Fil: Spotorno, Viviana Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina 2018-10-30T12:29:49Z 2018-10-30T12:29:49Z 2013-03-15 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0956566312005441 http://hdl.handle.net/20.500.12123/3739 0956-5663 https://doi.org/10.1016/j.bios.2012.08.020 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Biosensors and Bioelectronics 41 : 211-217 (March 2013) |
| spellingShingle | Clenbuterol Ganado Bovino Nanotecnología Inocuidad Alimentaria Medicamentos Neurotrópicos Cattle Nanotechnology Food Safety Neurotropic Drugs Inmunosensores Regiart, Daniel Matias Gaston Fernández Baldo, Martín Alejandro Spotorno, Viviana Graciela Bertolino, Franco Adrián Raba, Julio Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic micro particles as bio-affinity platform |
| title | Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic micro particles as bio-affinity platform |
| title_full | Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic micro particles as bio-affinity platform |
| title_fullStr | Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic micro particles as bio-affinity platform |
| title_full_unstemmed | Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic micro particles as bio-affinity platform |
| title_short | Ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic micro particles as bio-affinity platform |
| title_sort | ultra sensitive microfluidic immunosensor for determination of clenbuterol in bovine hair samples using electrodeposited gold nanoparticles and magnetic micro particles as bio affinity platform |
| topic | Clenbuterol Ganado Bovino Nanotecnología Inocuidad Alimentaria Medicamentos Neurotrópicos Cattle Nanotechnology Food Safety Neurotropic Drugs Inmunosensores |
| url | https://www.sciencedirect.com/science/article/pii/S0956566312005441 http://hdl.handle.net/20.500.12123/3739 https://doi.org/10.1016/j.bios.2012.08.020 |
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