Highly GC-rich heterochromatin in chili peppers (Capsicum-Solanaceae): A cytogenetic and molecular characterization
Capsicum comprises 35 species of chili peppers and five of them are cultivated worldwide as spices or vegetables. Diploid karyotypes based in x = 12 and x = 13 are common in Capsicum and the constitutive heterochromatin (cHet) is of particular interest in the genus since it is largely variable, part...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/3735 https://www.sciencedirect.com/science/article/pii/S0304423818303157?via%3Dihub#! https://doi.org/10.1016/j.scienta.2018.04.060 |
| _version_ | 1855035194249052160 |
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| author | Grabiele, Mauro Debat, Humberto Julio Scaldaferro, Marisel Analía Aguilera, Patricia Mabel Moscone, Eduardo Alberto Seijo, José Guillermo Ducasse, Daniel Adrian |
| author_browse | Aguilera, Patricia Mabel Debat, Humberto Julio Ducasse, Daniel Adrian Grabiele, Mauro Moscone, Eduardo Alberto Scaldaferro, Marisel Analía Seijo, José Guillermo |
| author_facet | Grabiele, Mauro Debat, Humberto Julio Scaldaferro, Marisel Analía Aguilera, Patricia Mabel Moscone, Eduardo Alberto Seijo, José Guillermo Ducasse, Daniel Adrian |
| author_sort | Grabiele, Mauro |
| collection | INTA Digital |
| description | Capsicum comprises 35 species of chili peppers and five of them are cultivated worldwide as spices or vegetables. Diploid karyotypes based in x = 12 and x = 13 are common in Capsicum and the constitutive heterochromatin (cHet) is of particular interest in the genus since it is largely variable, particularly its highly GC-rich fraction. However, the repetitive DNA components of this heterochromatic regions are unknown. Given the co-localization of rDNA loci with the CMA+DAPI– heterochromatic bands, we tested the hypothesis that the highly GC-rich cHet fraction is composed of the whole 18S-25S ribosomal DNA (rDNA) unit or some of its components. Here we report on a novel satellite for Capsicum and Solanaceae composed of the complete rDNA unit. We physically mapped six Capsicum derived specific 18S-25S rDNA probes that covered the entire span of the rDNA unit and analysed a DraI restriction product on eight chromosomally different taxa of Capsicum, representative of the major phylogenetic clades of chili peppers. The co-localization of every gene and spacer probes of the 18S-25S rDNA unit suggest their structural function as a major repetitive component of the highly GC-rich cHet in Capsicum species with x = 12. In addition, analyses of the clones derived from restriction assays in C. pubescens suggested that the differential functional status of 18S-25S rDNA loci (nucleolar organizer regions -NORs- or cHet) in this species is related to a divergence in a short sequence upstream the regulatory transcription initiation site (TIS) of the intergenic spacer (IGS). The results here provided evidence that an rDNA mega satellite played a significant role in the evolution of the karyotype features of x = 12 Capsicum species. The finding of a mega satellite family derived from the whole rDNA unit is a novelty for plant genomes |
| format | info:ar-repo/semantics/artículo |
| id | INTA3735 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA37352018-10-29T18:07:24Z Highly GC-rich heterochromatin in chili peppers (Capsicum-Solanaceae): A cytogenetic and molecular characterization Grabiele, Mauro Debat, Humberto Julio Scaldaferro, Marisel Analía Aguilera, Patricia Mabel Moscone, Eduardo Alberto Seijo, José Guillermo Ducasse, Daniel Adrian Heterocromatina Capsicum Hibridación Citogenetica Heterochromatin Hybridization Cytogenetics 18S-25S rDNA Nucleolar Organizer Region Fluorescence in Situ Hybridization Cytogenetic Analysis Capsicum comprises 35 species of chili peppers and five of them are cultivated worldwide as spices or vegetables. Diploid karyotypes based in x = 12 and x = 13 are common in Capsicum and the constitutive heterochromatin (cHet) is of particular interest in the genus since it is largely variable, particularly its highly GC-rich fraction. However, the repetitive DNA components of this heterochromatic regions are unknown. Given the co-localization of rDNA loci with the CMA+DAPI– heterochromatic bands, we tested the hypothesis that the highly GC-rich cHet fraction is composed of the whole 18S-25S ribosomal DNA (rDNA) unit or some of its components. Here we report on a novel satellite for Capsicum and Solanaceae composed of the complete rDNA unit. We physically mapped six Capsicum derived specific 18S-25S rDNA probes that covered the entire span of the rDNA unit and analysed a DraI restriction product on eight chromosomally different taxa of Capsicum, representative of the major phylogenetic clades of chili peppers. The co-localization of every gene and spacer probes of the 18S-25S rDNA unit suggest their structural function as a major repetitive component of the highly GC-rich cHet in Capsicum species with x = 12. In addition, analyses of the clones derived from restriction assays in C. pubescens suggested that the differential functional status of 18S-25S rDNA loci (nucleolar organizer regions -NORs- or cHet) in this species is related to a divergence in a short sequence upstream the regulatory transcription initiation site (TIS) of the intergenic spacer (IGS). The results here provided evidence that an rDNA mega satellite played a significant role in the evolution of the karyotype features of x = 12 Capsicum species. The finding of a mega satellite family derived from the whole rDNA unit is a novelty for plant genomes Instituto de Patología Vegetal Fil: Grabiele, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Scaldaferro, Marisel Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Aguilera, Patricia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina Fil: Moscone, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Seijo, Jose Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste; Argentina Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias. Laboratorio de Biología Molecular; Argentina 2018-10-29T17:47:35Z 2018-10-29T17:47:35Z 2018-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3735 https://www.sciencedirect.com/science/article/pii/S0304423818303157?via%3Dihub#! 0304-4238 https://doi.org/10.1016/j.scienta.2018.04.060 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Scientia horticulturae 238 : 391-399. (19 August 2018) |
| spellingShingle | Heterocromatina Capsicum Hibridación Citogenetica Heterochromatin Hybridization Cytogenetics 18S-25S rDNA Nucleolar Organizer Region Fluorescence in Situ Hybridization Cytogenetic Analysis Grabiele, Mauro Debat, Humberto Julio Scaldaferro, Marisel Analía Aguilera, Patricia Mabel Moscone, Eduardo Alberto Seijo, José Guillermo Ducasse, Daniel Adrian Highly GC-rich heterochromatin in chili peppers (Capsicum-Solanaceae): A cytogenetic and molecular characterization |
| title | Highly GC-rich heterochromatin in chili peppers (Capsicum-Solanaceae): A cytogenetic and molecular characterization |
| title_full | Highly GC-rich heterochromatin in chili peppers (Capsicum-Solanaceae): A cytogenetic and molecular characterization |
| title_fullStr | Highly GC-rich heterochromatin in chili peppers (Capsicum-Solanaceae): A cytogenetic and molecular characterization |
| title_full_unstemmed | Highly GC-rich heterochromatin in chili peppers (Capsicum-Solanaceae): A cytogenetic and molecular characterization |
| title_short | Highly GC-rich heterochromatin in chili peppers (Capsicum-Solanaceae): A cytogenetic and molecular characterization |
| title_sort | highly gc rich heterochromatin in chili peppers capsicum solanaceae a cytogenetic and molecular characterization |
| topic | Heterocromatina Capsicum Hibridación Citogenetica Heterochromatin Hybridization Cytogenetics 18S-25S rDNA Nucleolar Organizer Region Fluorescence in Situ Hybridization Cytogenetic Analysis |
| url | http://hdl.handle.net/20.500.12123/3735 https://www.sciencedirect.com/science/article/pii/S0304423818303157?via%3Dihub#! https://doi.org/10.1016/j.scienta.2018.04.060 |
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