In vitro selection and characterization of buffelgrass somaclones with different responses to water stress
Buffelgrass is a forage grass that reproduces mainly by apomixis. In species with this reproduction mode, in vitro selection allows the incorporation of alternatives in a breeding program. The aims were to define a protocol for in vitro selection, provide a molecular and morphological characterizati...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://link.springer.com/article/10.1007/s11240-017-1220-9 http://hdl.handle.net/20.500.12123/3716 https://doi.org/10.1007/s11240-017-1220-9 |
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| author | Carloni, Edgardo José Tommasino, Exequiel Arturo Lopez Colomba, Eliana Ribotta, Andrea Noemi Quiroga, Mariana Paola Griffa, Sabrina Mariana Grunberg, Karina |
| author_browse | Carloni, Edgardo José Griffa, Sabrina Mariana Grunberg, Karina Lopez Colomba, Eliana Quiroga, Mariana Paola Ribotta, Andrea Noemi Tommasino, Exequiel Arturo |
| author_facet | Carloni, Edgardo José Tommasino, Exequiel Arturo Lopez Colomba, Eliana Ribotta, Andrea Noemi Quiroga, Mariana Paola Griffa, Sabrina Mariana Grunberg, Karina |
| author_sort | Carloni, Edgardo José |
| collection | INTA Digital |
| description | Buffelgrass is a forage grass that reproduces mainly by apomixis. In species with this reproduction mode, in vitro selection allows the incorporation of alternatives in a breeding program. The aims were to define a protocol for in vitro selection, provide a molecular and morphological characterization of the progenies of regenerated plants, and evaluate them under water stress conditions. In the embryogenic callus induction medium (IM), the highest values of the variables fresh weight of embryogenic calli, proportion of embryogenic calli and number of regenerated seedlings (NRS) were obtained in the 25 mM mannitol treatment. The remaining concentrations of the osmotic agent (50, 75, 100 and 150 mM) had a negative effect on these variables. In the regeneration medium (RM), NRS was reduced at all mannitol concentrations. When embryogenic calli were induced and seedlings were regenerated maintaining mannitol concentrations in IM and RM, the highest NRS values were recorded at 25 mM mannitol. In vitro regenerated seedlings transplanted to an experimental plot exhibited different morphological characteristics from those of the anther donor plant. ISSR primers detected 22% of polymorphic bands and divergence between 0.20 and 0.37 in in vitro regenerated plants. Finally, water stress assays confirmed that S1 progenies exhibited a differential behavior from that of the parent material. Under 100 mM of mannitol used as selection pressure in IM or in both IM and RM, S1 progenies of two regenerated materials had higher height, fresh weight and dry weight at the end of water stress assay. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3716 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA37162018-10-26T11:50:08Z In vitro selection and characterization of buffelgrass somaclones with different responses to water stress Carloni, Edgardo José Tommasino, Exequiel Arturo Lopez Colomba, Eliana Ribotta, Andrea Noemi Quiroga, Mariana Paola Griffa, Sabrina Mariana Grunberg, Karina Cenchrus Ciliaris Genotipos Fitomejoramiento Experimentación in Vitro Variación Somaclonal Apomixis Estrés Osmótico Genotypes Plant Breeding In Vitro Experimentation Somaclonal Variation Osmotic Stress Pasto buffel Estrés Hídrico Buffel Grass Buffelgrass is a forage grass that reproduces mainly by apomixis. In species with this reproduction mode, in vitro selection allows the incorporation of alternatives in a breeding program. The aims were to define a protocol for in vitro selection, provide a molecular and morphological characterization of the progenies of regenerated plants, and evaluate them under water stress conditions. In the embryogenic callus induction medium (IM), the highest values of the variables fresh weight of embryogenic calli, proportion of embryogenic calli and number of regenerated seedlings (NRS) were obtained in the 25 mM mannitol treatment. The remaining concentrations of the osmotic agent (50, 75, 100 and 150 mM) had a negative effect on these variables. In the regeneration medium (RM), NRS was reduced at all mannitol concentrations. When embryogenic calli were induced and seedlings were regenerated maintaining mannitol concentrations in IM and RM, the highest NRS values were recorded at 25 mM mannitol. In vitro regenerated seedlings transplanted to an experimental plot exhibited different morphological characteristics from those of the anther donor plant. ISSR primers detected 22% of polymorphic bands and divergence between 0.20 and 0.37 in in vitro regenerated plants. Finally, water stress assays confirmed that S1 progenies exhibited a differential behavior from that of the parent material. Under 100 mM of mannitol used as selection pressure in IM or in both IM and RM, S1 progenies of two regenerated materials had higher height, fresh weight and dry weight at the end of water stress assay. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Tommasino, Exequiel Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Lopez Colomba, Eliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Ribotta, Andrea Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Quiroga, Mariana Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Griffa, Sabrina Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo de Nacional Investigaciones Científicas y Técnicas; Argentina 2018-10-26T11:48:22Z 2018-10-26T11:48:22Z 2017-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s11240-017-1220-9 http://hdl.handle.net/20.500.12123/3716 0167-6857 1573-5044 https://doi.org/10.1007/s11240-017-1220-9 eng info:eu-repo/semantics/restrictedAccess application/pdf Plant Cell, Tissue and Organ Culture 130 (2) : 265–277 (August 2017) |
| spellingShingle | Cenchrus Ciliaris Genotipos Fitomejoramiento Experimentación in Vitro Variación Somaclonal Apomixis Estrés Osmótico Genotypes Plant Breeding In Vitro Experimentation Somaclonal Variation Osmotic Stress Pasto buffel Estrés Hídrico Buffel Grass Carloni, Edgardo José Tommasino, Exequiel Arturo Lopez Colomba, Eliana Ribotta, Andrea Noemi Quiroga, Mariana Paola Griffa, Sabrina Mariana Grunberg, Karina In vitro selection and characterization of buffelgrass somaclones with different responses to water stress |
| title | In vitro selection and characterization of buffelgrass somaclones with different responses to water stress |
| title_full | In vitro selection and characterization of buffelgrass somaclones with different responses to water stress |
| title_fullStr | In vitro selection and characterization of buffelgrass somaclones with different responses to water stress |
| title_full_unstemmed | In vitro selection and characterization of buffelgrass somaclones with different responses to water stress |
| title_short | In vitro selection and characterization of buffelgrass somaclones with different responses to water stress |
| title_sort | in vitro selection and characterization of buffelgrass somaclones with different responses to water stress |
| topic | Cenchrus Ciliaris Genotipos Fitomejoramiento Experimentación in Vitro Variación Somaclonal Apomixis Estrés Osmótico Genotypes Plant Breeding In Vitro Experimentation Somaclonal Variation Osmotic Stress Pasto buffel Estrés Hídrico Buffel Grass |
| url | https://link.springer.com/article/10.1007/s11240-017-1220-9 http://hdl.handle.net/20.500.12123/3716 https://doi.org/10.1007/s11240-017-1220-9 |
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