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Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV)

A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical ino...

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Main Authors: Bejerman, Nicolas Esteban, Giolitti, Fabian, De Breuil, Soledad, Lenardon, Sergio Luis
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Springer; Virology Division, International Union of Microbiological Societies 2018
Subjects:
Helianthus Annuus
Virus de las Plantas
Clones
Potyvirus
Inoculación
Plant Viruses
Potyviruses
Inoculation
Girasol
Virus del Moteado Clorótico del Girasol
Sunflower
SuCMoV
Sunflower Chlorotic Mottle Virus
Infectious Clone
Online Access:http://hdl.handle.net/20.500.12123/3711
https://link.springer.com/article/10.1007/s00705-012-1497-2
Summary:A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome.

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