Differential salt-stress response during germination and vegetative growth in in vitro selected somaclonal mutants of Cenchrus ciliaris L.
Four somaclonal mutants (S1, S4, S6 and M10) and their parental Cenchrus ciliaris L. cultivar Biloela were characterized under salinity conditions at germination and vegetative growth stages. Seeds of all somaclonal mutants had higher germination percentages than cv. Biloela seeds in the control and...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S0254629913002184 http://hdl.handle.net/20.500.12123/3709 https://doi.org/10.1016/j.sajb.2013.03.008 |
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| author | Lopez Colomba, Eliana Tommasino, Exequiel Arturo Luna, Celina Mercedes Griffa, Sabrina Mariana Carloni, Edgardo José Ribotta, Andrea Noemi Quiroga, Mariana Paola Grunberg, Karina |
| author_browse | Carloni, Edgardo José Griffa, Sabrina Mariana Grunberg, Karina Lopez Colomba, Eliana Luna, Celina Mercedes Quiroga, Mariana Paola Ribotta, Andrea Noemi Tommasino, Exequiel Arturo |
| author_facet | Lopez Colomba, Eliana Tommasino, Exequiel Arturo Luna, Celina Mercedes Griffa, Sabrina Mariana Carloni, Edgardo José Ribotta, Andrea Noemi Quiroga, Mariana Paola Grunberg, Karina |
| author_sort | Lopez Colomba, Eliana |
| collection | INTA Digital |
| description | Four somaclonal mutants (S1, S4, S6 and M10) and their parental Cenchrus ciliaris L. cultivar Biloela were characterized under salinity conditions at germination and vegetative growth stages. Seeds of all somaclonal mutants had higher germination percentages than cv. Biloela seeds in the control and salt treatments. At 150 mM, germination was significantly higher in M10, S6 and S4 (72.3%, 66.3% and 61.8%, respectively) than in cv. Biloela (35.5%). Mutants grown under salinity along with cv. Biloela for 35 days had a different relative growth rate. S6 had the highest growth rate, indicating its potential tolerance to salt stress, whereas M10 was the most sensitive, with Bi, S4 and S1 being intermediate tolerant genotypes. Catalase enzyme activity (CAT) in M10 decreased in response to salt stress and was significantly associated with malondialdehide content, suggesting salt injury, whereas higher levels of CAT activity in S6 during salt stress were associated with increased salinity tolerance. The present results indicate that somaclonal variation and in vitro mutagenesis offer an effective tool for improvement of C. ciliaris because the somaclonal mutants showed differential tolerance to salt stress with respect to their parental and could be a better choice for use in a breeding program. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3709 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA37092018-10-25T15:00:00Z Differential salt-stress response during germination and vegetative growth in in vitro selected somaclonal mutants of Cenchrus ciliaris L. Lopez Colomba, Eliana Tommasino, Exequiel Arturo Luna, Celina Mercedes Griffa, Sabrina Mariana Carloni, Edgardo José Ribotta, Andrea Noemi Quiroga, Mariana Paola Grunberg, Karina Cenchrus Ciliaris Estrés Osmótico Variación Somaclonal Mutantes Germinación Periodo Vegetativo Osmotic Stress Somaclonal Variation Mutants Germination Vegetative Period Pasto buffel Buffel Grass Estres Salino Four somaclonal mutants (S1, S4, S6 and M10) and their parental Cenchrus ciliaris L. cultivar Biloela were characterized under salinity conditions at germination and vegetative growth stages. Seeds of all somaclonal mutants had higher germination percentages than cv. Biloela seeds in the control and salt treatments. At 150 mM, germination was significantly higher in M10, S6 and S4 (72.3%, 66.3% and 61.8%, respectively) than in cv. Biloela (35.5%). Mutants grown under salinity along with cv. Biloela for 35 days had a different relative growth rate. S6 had the highest growth rate, indicating its potential tolerance to salt stress, whereas M10 was the most sensitive, with Bi, S4 and S1 being intermediate tolerant genotypes. Catalase enzyme activity (CAT) in M10 decreased in response to salt stress and was significantly associated with malondialdehide content, suggesting salt injury, whereas higher levels of CAT activity in S6 during salt stress were associated with increased salinity tolerance. The present results indicate that somaclonal variation and in vitro mutagenesis offer an effective tool for improvement of C. ciliaris because the somaclonal mutants showed differential tolerance to salt stress with respect to their parental and could be a better choice for use in a breeding program. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Lopez Colomba, Eliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Tommasino, Exequiel Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Luna, Celina Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Griffa, Sabrina Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Ribotta, Andrea Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Quiroga, Mariana Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina 2018-10-25T14:58:40Z 2018-10-25T14:58:40Z 2013-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0254629913002184 http://hdl.handle.net/20.500.12123/3709 0254-6299 https://doi.org/10.1016/j.sajb.2013.03.008 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf South African Journal of Botany 87 : 157-163 (July 2013) |
| spellingShingle | Cenchrus Ciliaris Estrés Osmótico Variación Somaclonal Mutantes Germinación Periodo Vegetativo Osmotic Stress Somaclonal Variation Mutants Germination Vegetative Period Pasto buffel Buffel Grass Estres Salino Lopez Colomba, Eliana Tommasino, Exequiel Arturo Luna, Celina Mercedes Griffa, Sabrina Mariana Carloni, Edgardo José Ribotta, Andrea Noemi Quiroga, Mariana Paola Grunberg, Karina Differential salt-stress response during germination and vegetative growth in in vitro selected somaclonal mutants of Cenchrus ciliaris L. |
| title | Differential salt-stress response during germination and vegetative growth in in vitro selected somaclonal mutants of Cenchrus ciliaris L. |
| title_full | Differential salt-stress response during germination and vegetative growth in in vitro selected somaclonal mutants of Cenchrus ciliaris L. |
| title_fullStr | Differential salt-stress response during germination and vegetative growth in in vitro selected somaclonal mutants of Cenchrus ciliaris L. |
| title_full_unstemmed | Differential salt-stress response during germination and vegetative growth in in vitro selected somaclonal mutants of Cenchrus ciliaris L. |
| title_short | Differential salt-stress response during germination and vegetative growth in in vitro selected somaclonal mutants of Cenchrus ciliaris L. |
| title_sort | differential salt stress response during germination and vegetative growth in in vitro selected somaclonal mutants of cenchrus ciliaris l |
| topic | Cenchrus Ciliaris Estrés Osmótico Variación Somaclonal Mutantes Germinación Periodo Vegetativo Osmotic Stress Somaclonal Variation Mutants Germination Vegetative Period Pasto buffel Buffel Grass Estres Salino |
| url | https://www.sciencedirect.com/science/article/pii/S0254629913002184 http://hdl.handle.net/20.500.12123/3709 https://doi.org/10.1016/j.sajb.2013.03.008 |
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