First report of rice stripe necrosis virus in Argentina
Since 2008/2009, we have seen symptoms of viral infection in rice plants from crop regions in Argentina. The main objective of this work was to identify the causal agent of the rice plants' crinkling. We therefore analyzed samples of symptomatic rice plants from Berón de Astrada (Corrientes province...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/3704 https://www.sciencedirect.com/science/article/pii/S0261219418302163?via%3Dihub https://doi.org/10.1016/j.cropro.2018.08.012 |
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| author | Maurino, María Fernanda Gimenez Pecci, Maria de la Paz Kruger, Raúl Cúndom, María Águeda Gutierrez, Susana Alejandra Celli, Marcos Giovani |
| author_browse | Celli, Marcos Giovani Cúndom, María Águeda Gimenez Pecci, Maria de la Paz Gutierrez, Susana Alejandra Kruger, Raúl Maurino, María Fernanda |
| author_facet | Maurino, María Fernanda Gimenez Pecci, Maria de la Paz Kruger, Raúl Cúndom, María Águeda Gutierrez, Susana Alejandra Celli, Marcos Giovani |
| author_sort | Maurino, María Fernanda |
| collection | INTA Digital |
| description | Since 2008/2009, we have seen symptoms of viral infection in rice plants from crop regions in Argentina. The main objective of this work was to identify the causal agent of the rice plants' crinkling. We therefore analyzed samples of symptomatic rice plants from Berón de Astrada (Corrientes province) and San Javier (Santa Fe province) by serological, electron microscopy and RT-PCR methods, and determined the presence of Rice stripe necrosis virus (RSNV). Helically constructed rod-shaped virus particles, with an axial canal with an estimated modal length of 228 nm, 370 nm and a last interval of 87–181 nm and a diameter of 20 nm, were observed in ultrathin sections and ISEM-D from rice leaf samples. Transmission of RSNV by viruliferous soil was achieved. Phylogenetic analysis, based on a partial sequence (828 nt) of the helicase gene of 10 RSNV isolates, grouped the Argentinian sequences in the same clade as the African, rather than with the sequence from Colombia. This is the first report not only of the presence of RSNV in Argentina, but of a viral infection in rice plants in the country |
| format | info:ar-repo/semantics/artículo |
| id | INTA3704 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA37042019-01-14T17:21:45Z First report of rice stripe necrosis virus in Argentina Maurino, María Fernanda Gimenez Pecci, Maria de la Paz Kruger, Raúl Cúndom, María Águeda Gutierrez, Susana Alejandra Celli, Marcos Giovani Arroz Virus de las Plantas Oryza Sativa Técnicas Inmunológicas Rice Rice Stripe Virus Plant Viruses Immunological Techniques PCR Benyvirus RSNV Since 2008/2009, we have seen symptoms of viral infection in rice plants from crop regions in Argentina. The main objective of this work was to identify the causal agent of the rice plants' crinkling. We therefore analyzed samples of symptomatic rice plants from Berón de Astrada (Corrientes province) and San Javier (Santa Fe province) by serological, electron microscopy and RT-PCR methods, and determined the presence of Rice stripe necrosis virus (RSNV). Helically constructed rod-shaped virus particles, with an axial canal with an estimated modal length of 228 nm, 370 nm and a last interval of 87–181 nm and a diameter of 20 nm, were observed in ultrathin sections and ISEM-D from rice leaf samples. Transmission of RSNV by viruliferous soil was achieved. Phylogenetic analysis, based on a partial sequence (828 nt) of the helicase gene of 10 RSNV isolates, grouped the Argentinian sequences in the same clade as the African, rather than with the sequence from Colombia. This is the first report not only of the presence of RSNV in Argentina, but of a viral infection in rice plants in the country Instituto de Patología Vegetal Fil: Maurino, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gimenez, Maria de la Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Kruger, Raúl. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Corrientes; Argentina Fil: Cúndom, María Águeda. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina Fil: Gutierrez, Susana Alejandra. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-10-25T13:10:32Z 2018-10-25T13:10:32Z 2018-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3704 https://www.sciencedirect.com/science/article/pii/S0261219418302163?via%3Dihub 0261-2194 https://doi.org/10.1016/j.cropro.2018.08.012 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Crop protection 114 : 143-147. (December 2018) |
| spellingShingle | Arroz Virus de las Plantas Oryza Sativa Técnicas Inmunológicas Rice Rice Stripe Virus Plant Viruses Immunological Techniques PCR Benyvirus RSNV Maurino, María Fernanda Gimenez Pecci, Maria de la Paz Kruger, Raúl Cúndom, María Águeda Gutierrez, Susana Alejandra Celli, Marcos Giovani First report of rice stripe necrosis virus in Argentina |
| title | First report of rice stripe necrosis virus in Argentina |
| title_full | First report of rice stripe necrosis virus in Argentina |
| title_fullStr | First report of rice stripe necrosis virus in Argentina |
| title_full_unstemmed | First report of rice stripe necrosis virus in Argentina |
| title_short | First report of rice stripe necrosis virus in Argentina |
| title_sort | first report of rice stripe necrosis virus in argentina |
| topic | Arroz Virus de las Plantas Oryza Sativa Técnicas Inmunológicas Rice Rice Stripe Virus Plant Viruses Immunological Techniques PCR Benyvirus RSNV |
| url | http://hdl.handle.net/20.500.12123/3704 https://www.sciencedirect.com/science/article/pii/S0261219418302163?via%3Dihub https://doi.org/10.1016/j.cropro.2018.08.012 |
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