Cell remodeling and subtilase gene expression in the actinorhizal plant Discaria trinervis highlight host orchestration of intercellular Frankia colonization
Nitrogen‐fixing filamentous Frankia colonize the root tissues of its actinorhizal host Discaria trinervis via an exclusively intercellular pathway. Here we present studies aimed at uncovering mechanisms associated with this little‐researched mode of root entry, and in particular the extent to which...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | http://hdl.handle.net/20.500.12123/3693 https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.15216 https://doi.org/10.1111/nph.15216 |
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| author | Fournier, Joëlle Imanishi, Leandro Ezequiel Chabaud, Mireille Abdou‐Pavy, Iltaf Genre, Andrea Brichet, Lukas Lascano, Hernan Ramiro Muñoz, Nacira Belen Vayssières, Alice Pirolles, Elodie Brottier, Laurent Gherbi, Hassen Hocher, Valérie Svistoonoff, Sergio Barker, David G. Wall, Luis Gabriel |
| author_browse | Abdou‐Pavy, Iltaf Barker, David G. Brichet, Lukas Brottier, Laurent Chabaud, Mireille Fournier, Joëlle Genre, Andrea Gherbi, Hassen Hocher, Valérie Imanishi, Leandro Ezequiel Lascano, Hernan Ramiro Muñoz, Nacira Belen Pirolles, Elodie Svistoonoff, Sergio Vayssières, Alice Wall, Luis Gabriel |
| author_facet | Fournier, Joëlle Imanishi, Leandro Ezequiel Chabaud, Mireille Abdou‐Pavy, Iltaf Genre, Andrea Brichet, Lukas Lascano, Hernan Ramiro Muñoz, Nacira Belen Vayssières, Alice Pirolles, Elodie Brottier, Laurent Gherbi, Hassen Hocher, Valérie Svistoonoff, Sergio Barker, David G. Wall, Luis Gabriel |
| author_sort | Fournier, Joëlle |
| collection | INTA Digital |
| description | Nitrogen‐fixing filamentous Frankia colonize the root tissues of its actinorhizal host Discaria trinervis via an exclusively intercellular pathway. Here we present studies aimed at uncovering mechanisms associated with this little‐researched mode of root entry, and in particular the extent to which the host plant is an active partner during this process.
Detailed characterization of the expression patterns of infection‐associated actinorhizal host genes has provided valuable tools to identify intercellular infection sites, thus allowing in vivo confocal microscopic studies of the early stages of Frankia colonization.
The subtilisin‐like serine protease gene Dt12, as well as its Casuarina glauca homolog Cg12, are specifically expressed at sites of Frankia intercellular colonization of D. trinervis outer root tissues. This is accompanied by nucleo‐cytoplasmic reorganization in the adjacent host cells and major remodeling of the intercellular apoplastic compartment.
These findings lead us to propose that the actinorhizal host plays a major role in modifying both the size and composition of the intercellular apoplast in order to accommodate the filamentous microsymbiont. The implications of these findings are discussed in the light of the analogies that can be made with the orchestrating role of host legumes during intracellular root hair colonization by nitrogen‐fixing rhizobia. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3693 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA36932024-08-23T12:10:44Z Cell remodeling and subtilase gene expression in the actinorhizal plant Discaria trinervis highlight host orchestration of intercellular Frankia colonization Fournier, Joëlle Imanishi, Leandro Ezequiel Chabaud, Mireille Abdou‐Pavy, Iltaf Genre, Andrea Brichet, Lukas Lascano, Hernan Ramiro Muñoz, Nacira Belen Vayssières, Alice Pirolles, Elodie Brottier, Laurent Gherbi, Hassen Hocher, Valérie Svistoonoff, Sergio Barker, David G. Wall, Luis Gabriel Frankia Bacteria Bacteria Fijadora del Nitrógeno Expresión Génica Huéspedes Fijación Biológica del Nitrógeno Suelo Nitrogen Fixing Bacteria Gene Expression Hosts Biological Nitrogen Fixation Soil Discaria trinervis Nitrogen‐fixing filamentous Frankia colonize the root tissues of its actinorhizal host Discaria trinervis via an exclusively intercellular pathway. Here we present studies aimed at uncovering mechanisms associated with this little‐researched mode of root entry, and in particular the extent to which the host plant is an active partner during this process. Detailed characterization of the expression patterns of infection‐associated actinorhizal host genes has provided valuable tools to identify intercellular infection sites, thus allowing in vivo confocal microscopic studies of the early stages of Frankia colonization. The subtilisin‐like serine protease gene Dt12, as well as its Casuarina glauca homolog Cg12, are specifically expressed at sites of Frankia intercellular colonization of D. trinervis outer root tissues. This is accompanied by nucleo‐cytoplasmic reorganization in the adjacent host cells and major remodeling of the intercellular apoplastic compartment. These findings lead us to propose that the actinorhizal host plays a major role in modifying both the size and composition of the intercellular apoplast in order to accommodate the filamentous microsymbiont. The implications of these findings are discussed in the light of the analogies that can be made with the orchestrating role of host legumes during intracellular root hair colonization by nitrogen‐fixing rhizobia. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Fournier, Joëlle. Université de Toulouse. LIPM; Francia. INRA-CNRS; Francia Fil: Imanishi, Leandro Ezequiel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioquímica, Microbiología e Interacciones Biológicas en el Suelo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Chabaud, Mireille. Université de Toulouse. LIPM; Francia. INRA-CNRS; Francia Fil: Abdou‐Pavy, Iltaf. Université de Toulouse. LIPM; Francia. INRA-CNRS; Francia Fil: Genre, Andrea. University of Torino. Department of Life Sciences and Systems Biology; Italia Fil: Brichet, Lukas. Université de Toulouse. LIPM; Francia. INRA-CNRS; Francia Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Vayssières, Alice. IRD-INRA-CIRAD-Université de Montpellier-Supagro. Laboratoire des Symbioses Tropicales et Méditerranéennes; Francia Fil: Pirolles, Elodie. IRD-INRA-CIRAD-Université de Montpellier-Supagro. Laboratoire des Symbioses Tropicales et Méditerranéennes; Francia Fil: Brottier, Laurent. IRD-INRA-CIRAD-Université de Montpellier-Supagro. Laboratoire des Symbioses Tropicales et Méditerranéennes; Francia Fil: Gherbi, Hassen. IRD-INRA-CIRAD-Université de Montpellier-Supagro. Laboratoire des Symbioses Tropicales et Méditerranéennes; Francia Fil: Hocher, Valérie. IRD-INRA-CIRAD-Université de Montpellier-Supagro. Laboratoire des Symbioses Tropicales et Méditerranéennes; Francia Fil: Svistoonoff, Sergio. IRD-INRA-CIRAD-Université de Montpellier-Supagro. Laboratoire des Symbioses Tropicales et Méditerranéennes; Francia. Centre de Recherche de Bel Air. Laboratoire Mixte International Adaptation des Plantes et Microorganismes Associés aux Stress Environnementaux; Senegal. Institut de Recherche pour le Développement-Institut Sénégalais des Recherches Agricoles-Université Cheikh Anta Diop. Laboratoire Commun de Microbiologie; Senegal Fil: Barker, David G. Université de Toulouse. LIPM; Francia. INRA-CNRS; Francia Fil: Wall, Luis Gabriel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioquímica, Microbiología e Interacciones Biológicas en el Suelo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-10-24T14:10:15Z 2018-10-24T14:10:15Z 2018-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3693 https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.15216 0028-646X 1469-8137 https://doi.org/10.1111/nph.15216 eng info:eu-repo/semantics/restrictedAccess application/pdf New Phytologist 219 (3) : 1018-1030 (August 2018) |
| spellingShingle | Frankia Bacteria Bacteria Fijadora del Nitrógeno Expresión Génica Huéspedes Fijación Biológica del Nitrógeno Suelo Nitrogen Fixing Bacteria Gene Expression Hosts Biological Nitrogen Fixation Soil Discaria trinervis Fournier, Joëlle Imanishi, Leandro Ezequiel Chabaud, Mireille Abdou‐Pavy, Iltaf Genre, Andrea Brichet, Lukas Lascano, Hernan Ramiro Muñoz, Nacira Belen Vayssières, Alice Pirolles, Elodie Brottier, Laurent Gherbi, Hassen Hocher, Valérie Svistoonoff, Sergio Barker, David G. Wall, Luis Gabriel Cell remodeling and subtilase gene expression in the actinorhizal plant Discaria trinervis highlight host orchestration of intercellular Frankia colonization |
| title | Cell remodeling and subtilase gene expression in the actinorhizal plant Discaria trinervis highlight host orchestration of intercellular Frankia colonization |
| title_full | Cell remodeling and subtilase gene expression in the actinorhizal plant Discaria trinervis highlight host orchestration of intercellular Frankia colonization |
| title_fullStr | Cell remodeling and subtilase gene expression in the actinorhizal plant Discaria trinervis highlight host orchestration of intercellular Frankia colonization |
| title_full_unstemmed | Cell remodeling and subtilase gene expression in the actinorhizal plant Discaria trinervis highlight host orchestration of intercellular Frankia colonization |
| title_short | Cell remodeling and subtilase gene expression in the actinorhizal plant Discaria trinervis highlight host orchestration of intercellular Frankia colonization |
| title_sort | cell remodeling and subtilase gene expression in the actinorhizal plant discaria trinervis highlight host orchestration of intercellular frankia colonization |
| topic | Frankia Bacteria Bacteria Fijadora del Nitrógeno Expresión Génica Huéspedes Fijación Biológica del Nitrógeno Suelo Nitrogen Fixing Bacteria Gene Expression Hosts Biological Nitrogen Fixation Soil Discaria trinervis |
| url | http://hdl.handle.net/20.500.12123/3693 https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.15216 https://doi.org/10.1111/nph.15216 |
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