Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes
Modern breeding programs might have caused a reduction in plant responsiveness to arbuscular mycorrhizal fungi (AMF). Flavonoids and polyamines (PAs) are hypothesized to play a role in this symbiosis. We tested the effects of them in AMF roots of improved (I-1) and unimproved (UI-4) soybean genotype...
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2018
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| Online Access: | http://hdl.handle.net/20.500.12123/3675 https://link.springer.com/article/10.1007%2Fs13199-018-0558-z https://doi.org/10.1007/s13199-018-0558-z |
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| author | Salloum, María Soraya Menduni, Maria Florencia Benavides, María Patricia Larrauri, Mariana Luna, Celina Mercedes Silvente, Sonia Teresa |
| author_browse | Benavides, María Patricia Larrauri, Mariana Luna, Celina Mercedes Menduni, Maria Florencia Salloum, María Soraya Silvente, Sonia Teresa |
| author_facet | Salloum, María Soraya Menduni, Maria Florencia Benavides, María Patricia Larrauri, Mariana Luna, Celina Mercedes Silvente, Sonia Teresa |
| author_sort | Salloum, María Soraya |
| collection | INTA Digital |
| description | Modern breeding programs might have caused a reduction in plant responsiveness to arbuscular mycorrhizal fungi (AMF). Flavonoids and polyamines (PAs) are hypothesized to play a role in this symbiosis. We tested the effects of them in AMF roots of improved (I-1) and unimproved (UI-4) soybean genotypes, under the hypothesis that domestication decreased their concentration in roots, affecting AMF colonization, particularly arbuscule formation. After 20 days of treatment, AMF roots of UI-4 genotype had greater amount of total flavonoids/phenols and PAs while in I-1 genotype no differences were observed between roots of mycorrhizal (M) and non mycorrhizal (NM) plants. Exogenous application of flavonoids led to an increase in arbuscules in both genotypes. Improved-1 genotype needed higher levels of flavonoids to reach the percentage of mycorrhization achieved by UI-4 control. In regard to PAs, mycorrhizal plants of both genotypes had higher endogenous concentration than NM plants, however, the highest concentration, especially of putrescine (put) was in UI-4 M genotype. To check the participation of put in symbiosis we used RNAi silencing methodology. Down regulation of the GmADC transcript, involved in put formation, had a profound negative effect on mycorrhizal colonization and also affected the normal development of the plant. By contrast, down regulation of GmDAO, in which ADC transcript was expressed, arbuscule formation was similar to control plant. Our results suggest that mycorrhizal colonization is affected by soybean domestication particularly arbuscule formation and this effect seems to be mediated by the endogenous roots levels of flavonoids and PAs, especially put. |
| format | Artículo |
| id | INTA3675 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA36752022-08-17T13:42:09Z Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes Salloum, María Soraya Menduni, Maria Florencia Benavides, María Patricia Larrauri, Mariana Luna, Celina Mercedes Silvente, Sonia Teresa Soja Glycine Max Poliaminas Flavonoides Genotipos Micorrizas Arbusculares Soybeans Polyamines Flavonoids Genotypes Arbuscular Mycorrhiza Modern breeding programs might have caused a reduction in plant responsiveness to arbuscular mycorrhizal fungi (AMF). Flavonoids and polyamines (PAs) are hypothesized to play a role in this symbiosis. We tested the effects of them in AMF roots of improved (I-1) and unimproved (UI-4) soybean genotypes, under the hypothesis that domestication decreased their concentration in roots, affecting AMF colonization, particularly arbuscule formation. After 20 days of treatment, AMF roots of UI-4 genotype had greater amount of total flavonoids/phenols and PAs while in I-1 genotype no differences were observed between roots of mycorrhizal (M) and non mycorrhizal (NM) plants. Exogenous application of flavonoids led to an increase in arbuscules in both genotypes. Improved-1 genotype needed higher levels of flavonoids to reach the percentage of mycorrhization achieved by UI-4 control. In regard to PAs, mycorrhizal plants of both genotypes had higher endogenous concentration than NM plants, however, the highest concentration, especially of putrescine (put) was in UI-4 M genotype. To check the participation of put in symbiosis we used RNAi silencing methodology. Down regulation of the GmADC transcript, involved in put formation, had a profound negative effect on mycorrhizal colonization and also affected the normal development of the plant. By contrast, down regulation of GmDAO, in which ADC transcript was expressed, arbuscule formation was similar to control plant. Our results suggest that mycorrhizal colonization is affected by soybean domestication particularly arbuscule formation and this effect seems to be mediated by the endogenous roots levels of flavonoids and PAs, especially put. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Salloum, María Soraya. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Menduni, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Fondo para la Investigación Científica y Tecnológica; Argentina Fil: Benavides, María Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina Fil: Larrauri, Mariana. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Luna, Celina Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Silvente, Sonia. Universidad Nacional de Chilecito. Instituto de Ambiente de Montaña y Regiones Áridas (IAMRA); Argentina 2018-10-23T14:24:19Z 2018-10-23T14:24:19Z 2018-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3675 https://link.springer.com/article/10.1007%2Fs13199-018-0558-z 0334-5114 1878-7665 (Online) https://doi.org/10.1007/s13199-018-0558-z eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Symbiosis 76 (3) : 265–275. (November 2018) |
| spellingShingle | Soja Glycine Max Poliaminas Flavonoides Genotipos Micorrizas Arbusculares Soybeans Polyamines Flavonoids Genotypes Arbuscular Mycorrhiza Salloum, María Soraya Menduni, Maria Florencia Benavides, María Patricia Larrauri, Mariana Luna, Celina Mercedes Silvente, Sonia Teresa Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes |
| title | Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes |
| title_full | Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes |
| title_fullStr | Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes |
| title_full_unstemmed | Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes |
| title_short | Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes |
| title_sort | polyamines and flavonoids key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes |
| topic | Soja Glycine Max Poliaminas Flavonoides Genotipos Micorrizas Arbusculares Soybeans Polyamines Flavonoids Genotypes Arbuscular Mycorrhiza |
| url | http://hdl.handle.net/20.500.12123/3675 https://link.springer.com/article/10.1007%2Fs13199-018-0558-z https://doi.org/10.1007/s13199-018-0558-z |
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