Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
Bacillus amyloliquefaciens PGPBacCA1 was studied regarding its aptitude to protect common bean seeds from their intrinsic pathogens. Also, the inhibition of different environmental phytopathogenic fungi was tested. Two cultivars of Phaseolus vulgaris L. were evaluated: cv. Nag (black bean) and cv. A...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S1049964416302419 http://hdl.handle.net/20.500.12123/3674 https://doi.org/10.1016/j.biocontrol.2016.12.001 |
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| author | Torres, María Julia Perez Brandan, Carolina Sabate, Daniela Constanza Petroselli, Gabriela Erra Balsells, Rosa Audisio, Marcela Carina |
| author_browse | Audisio, Marcela Carina Erra Balsells, Rosa Perez Brandan, Carolina Petroselli, Gabriela Sabate, Daniela Constanza Torres, María Julia |
| author_facet | Torres, María Julia Perez Brandan, Carolina Sabate, Daniela Constanza Petroselli, Gabriela Erra Balsells, Rosa Audisio, Marcela Carina |
| author_sort | Torres, María Julia |
| collection | INTA Digital |
| description | Bacillus amyloliquefaciens PGPBacCA1 was studied regarding its aptitude to protect common bean seeds from their intrinsic pathogens. Also, the inhibition of different environmental phytopathogenic fungi was tested. Two cultivars of Phaseolus vulgaris L. were evaluated: cv. Nag (black bean) and cv. Alubia (white bean). Aspergillus spp., Penicillium spp. and Fusarium spp. constituted the natural fungal biota of both seeds, whereas white bean and black bean also exhibited Cladosporium spp. and Rhizopus spp., respectively. B. amyloliquefaciens PGPBacCA1 prevented the development of the endophytic fungi of black bean, while only Cladosporium spp. survived in the white variety. Growth chamber assays were carried out and bacilli cells were applied on seeds without affecting neither the vigor nor the germination potential of either type of bean. In addition, B. amyloliquefaciens PGPBacCA1, by dual cultures, was able to inhibit the development of the following phytopathogenic fungi: Sclerotium rolfsii (35%), Sclerotinia sclerotiorum (76.5%), Rhizoctonia solani (73%), Fusarium solani (56.5%), and Penicillium spp. (71.5%). The UV-MALDI TOF MS analysis showed that B. amyloliquefaciens PGPBacCA1 co-produces different homologues of the lipopeptides surfactin, iturin and fengycin in the presence of S. sclerotiorum and F. solani. These compounds were identified as the main responsible for the antagonistic effect. SEM analysis confirmed the antifungal effects of the lipopeptides, which also caused damage to chlamydospores and sclerotia of Fusarium and Sclerotinia, respectively. B. amyloliquefaciens PGPBacCA1 can thus be applied to these bean seeds varieties as a potential bioprotection agent. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3674 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA36742018-10-23T13:52:58Z Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens Torres, María Julia Perez Brandan, Carolina Sabate, Daniela Constanza Petroselli, Gabriela Erra Balsells, Rosa Audisio, Marcela Carina Bacillus Phaseolus vulgaris Fríjol (phaseolus) Fusarium Sclerotinia sclerotiorum Péptidos Kidney Beans Peptides Bacillus amyloliquefaciens Poroto Fusarium Solani Lipopeptidos Bacillus amyloliquefaciens PGPBacCA1 was studied regarding its aptitude to protect common bean seeds from their intrinsic pathogens. Also, the inhibition of different environmental phytopathogenic fungi was tested. Two cultivars of Phaseolus vulgaris L. were evaluated: cv. Nag (black bean) and cv. Alubia (white bean). Aspergillus spp., Penicillium spp. and Fusarium spp. constituted the natural fungal biota of both seeds, whereas white bean and black bean also exhibited Cladosporium spp. and Rhizopus spp., respectively. B. amyloliquefaciens PGPBacCA1 prevented the development of the endophytic fungi of black bean, while only Cladosporium spp. survived in the white variety. Growth chamber assays were carried out and bacilli cells were applied on seeds without affecting neither the vigor nor the germination potential of either type of bean. In addition, B. amyloliquefaciens PGPBacCA1, by dual cultures, was able to inhibit the development of the following phytopathogenic fungi: Sclerotium rolfsii (35%), Sclerotinia sclerotiorum (76.5%), Rhizoctonia solani (73%), Fusarium solani (56.5%), and Penicillium spp. (71.5%). The UV-MALDI TOF MS analysis showed that B. amyloliquefaciens PGPBacCA1 co-produces different homologues of the lipopeptides surfactin, iturin and fengycin in the presence of S. sclerotiorum and F. solani. These compounds were identified as the main responsible for the antagonistic effect. SEM analysis confirmed the antifungal effects of the lipopeptides, which also caused damage to chlamydospores and sclerotia of Fusarium and Sclerotinia, respectively. B. amyloliquefaciens PGPBacCA1 can thus be applied to these bean seeds varieties as a potential bioprotection agent. EEA Salta Fil: Torres, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina Fil: Pérez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Sabate, Daniela Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; Argentina Fil: Petroselli, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Erra Balsells, Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química; Argentina. Universidad Nacional de Salta. Instituto de Investigación para la Industria Química; Argentina 2018-10-23T13:50:35Z 2018-10-23T13:50:35Z 2017-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S1049964416302419 http://hdl.handle.net/20.500.12123/3674 1049-9644 https://doi.org/10.1016/j.biocontrol.2016.12.001 eng info:eu-repo/semantics/restrictedAccess application/pdf Biological Control 105 : 93-99 (February 2017) |
| spellingShingle | Bacillus Phaseolus vulgaris Fríjol (phaseolus) Fusarium Sclerotinia sclerotiorum Péptidos Kidney Beans Peptides Bacillus amyloliquefaciens Poroto Fusarium Solani Lipopeptidos Torres, María Julia Perez Brandan, Carolina Sabate, Daniela Constanza Petroselli, Gabriela Erra Balsells, Rosa Audisio, Marcela Carina Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens |
| title | Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens |
| title_full | Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens |
| title_fullStr | Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens |
| title_full_unstemmed | Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens |
| title_short | Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens |
| title_sort | biological activity of the lipopeptide producing bacillus amyloliquefaciens pgpbacca1 on common bean phaseolus vulgaris l pathogens |
| topic | Bacillus Phaseolus vulgaris Fríjol (phaseolus) Fusarium Sclerotinia sclerotiorum Péptidos Kidney Beans Peptides Bacillus amyloliquefaciens Poroto Fusarium Solani Lipopeptidos |
| url | https://www.sciencedirect.com/science/article/pii/S1049964416302419 http://hdl.handle.net/20.500.12123/3674 https://doi.org/10.1016/j.biocontrol.2016.12.001 |
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