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Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates

ESAT-6, CFP-10 and EspC are virulence factors that have been extensively assayed for bovine and human tuberculosis diagnosis due their potent T-cell inducing activities. While polymorphisms of ESAT-6 and CFP-10 were analyzed, with the description of CFP-10 variants in M. tuberculosis, this fact has...

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Main Authors: Encinas, Micaela, Marfil, Maria Jimena, Garbaccio, Sergio Gabriel, Barandiaran, Soledad, Huertas, Pablo Sebastian, Morsella, Claudia Graciela, Macias, Analía, Magnano, Gabriel, Zapata, L., Bigi, Fabiana, Cataldi, Angel Adrian, Paolicchi, Fernando, Zumarraga, Martin Jose, Eirin, Maria Emilia
Format: Artículo
Language:Inglés
Published: Elsevier 2018
Subjects:
Mycobacterium bovis Infections
Polymorphism
Antigens
Isolation Techniques
Infección por Mycobacterium bovis
Polimorfismo
Mycobacterium bovis
Antígenos
Técnicas de Aislamiento
Bovine Tuberculosis
Tuberculosis Bovina
Online Access:https://www.sciencedirect.com/science/article/pii/S1472979217304924?via%3Dihub
http://hdl.handle.net/20.500.12123/3664
https://doi.org/10.1016/j.tube.2018.06.007
Summary:ESAT-6, CFP-10 and EspC are virulence factors that have been extensively assayed for bovine and human tuberculosis diagnosis due their potent T-cell inducing activities. While polymorphisms of ESAT-6 and CFP-10 were analyzed, with the description of CFP-10 variants in M. tuberculosis, this fact has not been explored in M. bovis field isolates. The coding sequences of esxA (ESAT-6), esxB (CFP-10) and mb3645c (EspC) from 58 M. bovis strains exhibiting genomic variability (spoligotyping) were analyzed. Two genes –esxA and esxB – remained invariant while mb3645c exhibited one synonymous polymorphism (G to A mutation, position 66bp) in one isolate, compared to M. bovis AF2122/97 reference strain. All isolates exhibited a synonymous nucleotide polymorphism simultaneously (G to A mutation, position 255bp), compared to M. tuberculosis H37Rv reference strain. This study confirms the high conservation for ESAT-6, CFP-10 and EspC in local M. bovis field isolates and reinforce the use of these three antigens in the diagnosis of bovine tuberculosis. Further studies should be performed to globally confirm these findings.

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