Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates
ESAT-6, CFP-10 and EspC are virulence factors that have been extensively assayed for bovine and human tuberculosis diagnosis due their potent T-cell inducing activities. While polymorphisms of ESAT-6 and CFP-10 were analyzed, with the description of CFP-10 variants in M. tuberculosis, this fact has...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2018
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| Online Access: | https://www.sciencedirect.com/science/article/pii/S1472979217304924?via%3Dihub http://hdl.handle.net/20.500.12123/3664 https://doi.org/10.1016/j.tube.2018.06.007 |
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| author | Encinas, Micaela Marfil, Maria Jimena Garbaccio, Sergio Gabriel Barandiaran, Soledad Huertas, Pablo Sebastian Morsella, Claudia Graciela Macias, Analía Magnano, Gabriel Zapata, L. Bigi, Fabiana Cataldi, Angel Adrian Paolicchi, Fernando Zumarraga, Martin Jose Eirin, Maria Emilia |
| author_browse | Barandiaran, Soledad Bigi, Fabiana Cataldi, Angel Adrian Eirin, Maria Emilia Encinas, Micaela Garbaccio, Sergio Gabriel Huertas, Pablo Sebastian Macias, Analía Magnano, Gabriel Marfil, Maria Jimena Morsella, Claudia Graciela Paolicchi, Fernando Zapata, L. Zumarraga, Martin Jose |
| author_facet | Encinas, Micaela Marfil, Maria Jimena Garbaccio, Sergio Gabriel Barandiaran, Soledad Huertas, Pablo Sebastian Morsella, Claudia Graciela Macias, Analía Magnano, Gabriel Zapata, L. Bigi, Fabiana Cataldi, Angel Adrian Paolicchi, Fernando Zumarraga, Martin Jose Eirin, Maria Emilia |
| author_sort | Encinas, Micaela |
| collection | INTA Digital |
| description | ESAT-6, CFP-10 and EspC are virulence factors that have been extensively assayed for bovine and human tuberculosis diagnosis due their potent T-cell inducing activities. While polymorphisms of ESAT-6 and CFP-10 were analyzed, with the description of CFP-10 variants in M. tuberculosis, this fact has not been explored in M. bovis field isolates. The coding sequences of esxA (ESAT-6), esxB (CFP-10) and mb3645c (EspC) from 58 M. bovis strains exhibiting genomic variability (spoligotyping) were analyzed. Two genes –esxA and esxB – remained invariant while mb3645c exhibited one synonymous polymorphism (G to A mutation, position 66bp) in one isolate, compared to M. bovis AF2122/97 reference strain. All isolates exhibited a synonymous nucleotide polymorphism simultaneously (G to A mutation, position 255bp), compared to M. tuberculosis H37Rv reference strain. This study confirms the high conservation for ESAT-6, CFP-10 and EspC in local M. bovis field isolates and reinforce the use of these three antigens in the diagnosis of bovine tuberculosis. Further studies should be performed to globally confirm these findings. |
| format | Artículo |
| id | INTA3664 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA36642019-06-11T14:47:00Z Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates Encinas, Micaela Marfil, Maria Jimena Garbaccio, Sergio Gabriel Barandiaran, Soledad Huertas, Pablo Sebastian Morsella, Claudia Graciela Macias, Analía Magnano, Gabriel Zapata, L. Bigi, Fabiana Cataldi, Angel Adrian Paolicchi, Fernando Zumarraga, Martin Jose Eirin, Maria Emilia Mycobacterium bovis Infections Polymorphism Antigens Isolation Techniques Infección por Mycobacterium bovis Polimorfismo Mycobacterium bovis Antígenos Técnicas de Aislamiento Bovine Tuberculosis Tuberculosis Bovina ESAT-6, CFP-10 and EspC are virulence factors that have been extensively assayed for bovine and human tuberculosis diagnosis due their potent T-cell inducing activities. While polymorphisms of ESAT-6 and CFP-10 were analyzed, with the description of CFP-10 variants in M. tuberculosis, this fact has not been explored in M. bovis field isolates. The coding sequences of esxA (ESAT-6), esxB (CFP-10) and mb3645c (EspC) from 58 M. bovis strains exhibiting genomic variability (spoligotyping) were analyzed. Two genes –esxA and esxB – remained invariant while mb3645c exhibited one synonymous polymorphism (G to A mutation, position 66bp) in one isolate, compared to M. bovis AF2122/97 reference strain. All isolates exhibited a synonymous nucleotide polymorphism simultaneously (G to A mutation, position 255bp), compared to M. tuberculosis H37Rv reference strain. This study confirms the high conservation for ESAT-6, CFP-10 and EspC in local M. bovis field isolates and reinforce the use of these three antigens in the diagnosis of bovine tuberculosis. Further studies should be performed to globally confirm these findings. Instituto de Biotecnología Fil: Encinas, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Marfil, María Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Huertas, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Macias, Analía. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Magnano, Gabriel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Zapata, L. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Paoliochi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Eirin, Maria Emilia.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-10-23T11:02:39Z 2018-10-23T11:02:39Z 2018-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S1472979217304924?via%3Dihub http://hdl.handle.net/20.500.12123/3664 1472-9792 https://doi.org/10.1016/j.tube.2018.06.007 eng info:eu-repograntAgreement/INTA/PNBIO/1131034/AR./Inmunología molecular y genómica funcional aplicadas a interacciones patógeno hospedador de interés pecuario. info:eu-repograntAgreement/INTA/PNSA/1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional. info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Tuberculosis 111: 143–146 (Julio 2018) |
| spellingShingle | Mycobacterium bovis Infections Polymorphism Antigens Isolation Techniques Infección por Mycobacterium bovis Polimorfismo Mycobacterium bovis Antígenos Técnicas de Aislamiento Bovine Tuberculosis Tuberculosis Bovina Encinas, Micaela Marfil, Maria Jimena Garbaccio, Sergio Gabriel Barandiaran, Soledad Huertas, Pablo Sebastian Morsella, Claudia Graciela Macias, Analía Magnano, Gabriel Zapata, L. Bigi, Fabiana Cataldi, Angel Adrian Paolicchi, Fernando Zumarraga, Martin Jose Eirin, Maria Emilia Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates |
| title | Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates |
| title_full | Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates |
| title_fullStr | Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates |
| title_full_unstemmed | Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates |
| title_short | Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates |
| title_sort | mycobacterium bovis esat 6 cfp 10 and espc antigens show high conservation among field isolates |
| topic | Mycobacterium bovis Infections Polymorphism Antigens Isolation Techniques Infección por Mycobacterium bovis Polimorfismo Mycobacterium bovis Antígenos Técnicas de Aislamiento Bovine Tuberculosis Tuberculosis Bovina |
| url | https://www.sciencedirect.com/science/article/pii/S1472979217304924?via%3Dihub http://hdl.handle.net/20.500.12123/3664 https://doi.org/10.1016/j.tube.2018.06.007 |
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