Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina
Background: The main pathogen of neonatal and post weaning diarrhea and edema disease (ED) is Escherichia coli and pathotypes involved are enterotoxigenic, enteropathogenic, and shiga toxigenic (ETEC, EPEC, and STEC, respectively). Those diseases cause economic loss in pig production. Aim: The aim...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/3656 http://www.veterinaryworld.org/Vol.10/August-2017/18.html https://doi.org/10.14202/vetworld.2017.939-945 |
| _version_ | 1855035180136267776 |
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| author | Bessone, Fernando Aníbal Bessone, Gabriela Marini, Sebastián José Conde, María Belén Alustiza, Fabrisio Eduardo Zielinski, Gustavo Carlos |
| author_browse | Alustiza, Fabrisio Eduardo Bessone, Fernando Aníbal Bessone, Gabriela Conde, María Belén Marini, Sebastián José Zielinski, Gustavo Carlos |
| author_facet | Bessone, Fernando Aníbal Bessone, Gabriela Marini, Sebastián José Conde, María Belén Alustiza, Fabrisio Eduardo Zielinski, Gustavo Carlos |
| author_sort | Bessone, Fernando Aníbal |
| collection | INTA Digital |
| description | Background: The main pathogen of neonatal and post weaning diarrhea and edema disease (ED) is Escherichia coli and pathotypes involved are enterotoxigenic, enteropathogenic, and shiga toxigenic (ETEC, EPEC, and STEC, respectively). Those diseases cause economic loss in pig production.
Aim: The aim of this work was to evaluate the presence of strains expressing virulence markers genes and the antibiotic susceptibility profiles of E. coli from clinical cases of post weaning diarrhea and ED in farms in the central area of Argentina.
Materials and Methods: Intensive pig farms from the central region of Argentina were sampled. Intestinal mucosa swabs from pigs with diarrhea were taken, seeded on MacConkey agar plates, biochemically typified and tested by polymerase chain reaction (PCR). Antibiograms were made by disk-diffusion method.
Results: A total of 54 strains from clinical cases studied showed PCR findings: 88.88% (48/54) expressed at least one gene coding for a virulence factor. Colonization factors found were: 39.58% of strains had F18, 33.33% were F4 and 31.25% adhesin involved in diffuse adherence-I; 29.17%, 25%, and 2.1% expressed LT, STb, and STa, respectively. 25% were STx and 16.67% were eae positive. Only 2.1% were STx2. The most active antibiotics against most strains were gentamicin and ceftiofur, but resistance profiles against many antibiotics were found.
Conclusion: High circulation of pathogens strains of E. coli among pigs with diarrhea with an extended antibiotic resistance profile. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3656 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA36562024-03-15T15:05:03Z Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina Bessone, Fernando Aníbal Bessone, Gabriela Marini, Sebastián José Conde, María Belén Alustiza, Fabrisio Eduardo Zielinski, Gustavo Carlos Cerdo Enfermedades de los Animales Escherichia coli Virulencia Resistencia a los Antibióticos Genética Swine Animal Diseases Virulence Resistance to Antibiotics Genetics Argentina Background: The main pathogen of neonatal and post weaning diarrhea and edema disease (ED) is Escherichia coli and pathotypes involved are enterotoxigenic, enteropathogenic, and shiga toxigenic (ETEC, EPEC, and STEC, respectively). Those diseases cause economic loss in pig production. Aim: The aim of this work was to evaluate the presence of strains expressing virulence markers genes and the antibiotic susceptibility profiles of E. coli from clinical cases of post weaning diarrhea and ED in farms in the central area of Argentina. Materials and Methods: Intensive pig farms from the central region of Argentina were sampled. Intestinal mucosa swabs from pigs with diarrhea were taken, seeded on MacConkey agar plates, biochemically typified and tested by polymerase chain reaction (PCR). Antibiograms were made by disk-diffusion method. Results: A total of 54 strains from clinical cases studied showed PCR findings: 88.88% (48/54) expressed at least one gene coding for a virulence factor. Colonization factors found were: 39.58% of strains had F18, 33.33% were F4 and 31.25% adhesin involved in diffuse adherence-I; 29.17%, 25%, and 2.1% expressed LT, STb, and STa, respectively. 25% were STx and 16.67% were eae positive. Only 2.1% were STx2. The most active antibiotics against most strains were gentamicin and ceftiofur, but resistance profiles against many antibiotics were found. Conclusion: High circulation of pathogens strains of E. coli among pigs with diarrhea with an extended antibiotic resistance profile. EEA Marcos Juárez Fil: Bessone, Fernando Aníbal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Bessone, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Marini, Sebastián José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Conde, María Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Zielinski, Gustavo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina 2018-10-22T11:49:09Z 2018-10-22T11:49:09Z 2017-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3656 http://www.veterinaryworld.org/Vol.10/August-2017/18.html 0972-8988 2231-0916 https://doi.org/10.14202/vetworld.2017.939-945 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Veterinary World 10 (8) : 939-945 (August 2017) |
| spellingShingle | Cerdo Enfermedades de los Animales Escherichia coli Virulencia Resistencia a los Antibióticos Genética Swine Animal Diseases Virulence Resistance to Antibiotics Genetics Argentina Bessone, Fernando Aníbal Bessone, Gabriela Marini, Sebastián José Conde, María Belén Alustiza, Fabrisio Eduardo Zielinski, Gustavo Carlos Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina |
| title | Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina |
| title_full | Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina |
| title_fullStr | Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina |
| title_full_unstemmed | Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina |
| title_short | Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina |
| title_sort | presence and characterization of escherichia coli virulence genes isolated from diseased pigs in the central region of argentina |
| topic | Cerdo Enfermedades de los Animales Escherichia coli Virulencia Resistencia a los Antibióticos Genética Swine Animal Diseases Virulence Resistance to Antibiotics Genetics Argentina |
| url | http://hdl.handle.net/20.500.12123/3656 http://www.veterinaryworld.org/Vol.10/August-2017/18.html https://doi.org/10.14202/vetworld.2017.939-945 |
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