Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina
Cassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and the causal agent, Cassava common mosaic virus (CsCMV), has been identified as a mechanically transmitted potexvirus (Alphaflexiviridae). In Argentina, cassava is grown mainly in the nor...
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/3652 https://onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12869 https://doi.org/10.1111/ppa.12869 |
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| author | Zanini, Andrea Alejandra Cuellar, Wilmer J. Celli, Marcos Giovani Luque, Andres Vicente Medina, Ricardo Daniel Conci, Vilma Cecilia Di Feo, Liliana Del Valle |
| author_browse | Celli, Marcos Giovani Conci, Vilma Cecilia Cuellar, Wilmer J. Di Feo, Liliana Del Valle Luque, Andres Vicente Medina, Ricardo Daniel Zanini, Andrea Alejandra |
| author_facet | Zanini, Andrea Alejandra Cuellar, Wilmer J. Celli, Marcos Giovani Luque, Andres Vicente Medina, Ricardo Daniel Conci, Vilma Cecilia Di Feo, Liliana Del Valle |
| author_sort | Zanini, Andrea Alejandra |
| collection | INTA Digital |
| description | Cassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and
the causal agent, Cassava common mosaic virus (CsCMV), has been identified as a mechanically transmitted potexvirus
(Alphaflexiviridae). In Argentina, cassava is grown mainly in the northeast (NEA) region that shares borders with Brazil
and Paraguay. Increasing incidences of CCMD were observed during the years 2014 to 2016 associated with severe leaf
mosaic symptoms and yield reductions where the occurrence of CsCMV was confirmed by RT-PCR and sequencing. In
this work, the virus has been successfully purified and a double-antibody sandwich (DAS-) ELISA test has been developed
from an Argentinean isolate of CsCMV to extend the diagnostics of the disease. A collection of 726 samples was screened
and CsCMV was detected with 100% prevalence in the NEA region. Additional co-infecting viruses were detected in
some plants (64.4%); in these, CCMD symptoms correlated with CsCMV only, although more severe symptoms could
be observed in mixed infected plants. Sequence analysis of the conserved RdRp domain showed a wider diversity of
CsCMV isolates. Interestingly, a separate phylogenetic cluster was formed by isolates from the NEA region that only
shared 77.1% to 80.3% nucleotide identity with the other clusters. These results indicate the presence of mixed strains
occurring in the NEA region and suggest the presence of geographically distinct strains of CsCMV in South America. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3652 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA36522018-10-19T18:11:30Z Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina Zanini, Andrea Alejandra Cuellar, Wilmer J. Celli, Marcos Giovani Luque, Andres Vicente Medina, Ricardo Daniel Conci, Vilma Cecilia Di Feo, Liliana Del Valle Mandioca Cassava Manihot Esculenta Virus de las Plantas Plant Viruses African Cassava Mosaic Virus Geminivirus Latente de la Yuca Africana Vigilancia de Enfermedades Disease Surveillance Cassava Common Mosaic Disease Argentina Cassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and the causal agent, Cassava common mosaic virus (CsCMV), has been identified as a mechanically transmitted potexvirus (Alphaflexiviridae). In Argentina, cassava is grown mainly in the northeast (NEA) region that shares borders with Brazil and Paraguay. Increasing incidences of CCMD were observed during the years 2014 to 2016 associated with severe leaf mosaic symptoms and yield reductions where the occurrence of CsCMV was confirmed by RT-PCR and sequencing. In this work, the virus has been successfully purified and a double-antibody sandwich (DAS-) ELISA test has been developed from an Argentinean isolate of CsCMV to extend the diagnostics of the disease. A collection of 726 samples was screened and CsCMV was detected with 100% prevalence in the NEA region. Additional co-infecting viruses were detected in some plants (64.4%); in these, CCMD symptoms correlated with CsCMV only, although more severe symptoms could be observed in mixed infected plants. Sequence analysis of the conserved RdRp domain showed a wider diversity of CsCMV isolates. Interestingly, a separate phylogenetic cluster was formed by isolates from the NEA region that only shared 77.1% to 80.3% nucleotide identity with the other clusters. These results indicate the presence of mixed strains occurring in the NEA region and suggest the presence of geographically distinct strains of CsCMV in South America. Instituto de Patología Vegetal Fil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cuellar, Wilmer J. International Center for Tropical Agriculture. Agrobiodiversity Research Area. Virology Laboratory; Colombia. Consortium of International Agricultural Research Centers. Research Program on Roots Tubers and Bananas; Colombia Fil: Celli, Marcos Giovani. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Luque, Andres Vicente. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Di Feo, Liliana Del Valle. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-10-19T18:03:00Z 2018-10-19T18:03:00Z 2018-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3652 https://onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12869 1365-3059 https://doi.org/10.1111/ppa.12869 eng info:eu-repo/semantics/restrictedAccess application/pdf Argentina (nation) Wiley Plant pathology 67 (8) : 1814-1820. (October 2018) |
| spellingShingle | Mandioca Cassava Manihot Esculenta Virus de las Plantas Plant Viruses African Cassava Mosaic Virus Geminivirus Latente de la Yuca Africana Vigilancia de Enfermedades Disease Surveillance Cassava Common Mosaic Disease Argentina Zanini, Andrea Alejandra Cuellar, Wilmer J. Celli, Marcos Giovani Luque, Andres Vicente Medina, Ricardo Daniel Conci, Vilma Cecilia Di Feo, Liliana Del Valle Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina |
| title | Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina |
| title_full | Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina |
| title_fullStr | Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina |
| title_full_unstemmed | Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina |
| title_short | Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina |
| title_sort | distinct strains of the re emergent cassava common mosaic virus genus potexvirus infecting cassava in argentina |
| topic | Mandioca Cassava Manihot Esculenta Virus de las Plantas Plant Viruses African Cassava Mosaic Virus Geminivirus Latente de la Yuca Africana Vigilancia de Enfermedades Disease Surveillance Cassava Common Mosaic Disease Argentina |
| url | http://hdl.handle.net/20.500.12123/3652 https://onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12869 https://doi.org/10.1111/ppa.12869 |
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