Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba
Luffa aegyptiaca (sponge gourd) plants showing severe leaf mosaic and deformation symptoms were observed in Villa Clara, Cuba. Electron microscopy observations of leaf dip preparations revealed flexuous filamentous particles, which were identified as Papaya ringspot virus (PRSV) by ELISA test. PRSV...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/3647 https://link.springer.com/article/10.1007%2Fs13314-017-0229-6#citeas https://doi.org/10.1007/s13314-017-0229-6 |
| _version_ | 1855035178487906304 |
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| author | Cabrera Mederos, Dariel Giolitti, Fabian Nome Docampo, Claudia Bejerman, Nicolas Esteban Portal, Orelvis |
| author_browse | Bejerman, Nicolas Esteban Cabrera Mederos, Dariel Giolitti, Fabian Nome Docampo, Claudia Portal, Orelvis |
| author_facet | Cabrera Mederos, Dariel Giolitti, Fabian Nome Docampo, Claudia Bejerman, Nicolas Esteban Portal, Orelvis |
| author_sort | Cabrera Mederos, Dariel |
| collection | INTA Digital |
| description | Luffa aegyptiaca (sponge gourd) plants showing severe leaf mosaic and deformation symptoms were observed in Villa Clara, Cuba. Electron microscopy observations of leaf dip preparations revealed flexuous filamentous particles, which were identified as Papaya ringspot virus (PRSV) by ELISA test. PRSV was mechanically transmitted to healthy Cucurbita moschata plants. Sequence analysis of the RT-PCR product obtained from the capsid protein gene showed highest identity with other PRSV isolates from United States of America, Australia, Venezuela, Brazil, India and Cuba, ranging between 93.2–96.5% and 93.4–98.1% for nucleotide and amino acid sequences, respectively. Phylogenetic analysis showed two main clusters. Cluster I included isolates from the Americas-Australia group and India, including sponge gourd isolate, while the cluster II included isolates from China, Thailand and Taiwan. To our knowledge, this is the first report of PRSV-W infecting sponge gourd in Cuba. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3647 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA36472018-10-19T15:02:05Z Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba Cabrera Mederos, Dariel Giolitti, Fabian Nome Docampo, Claudia Bejerman, Nicolas Esteban Portal, Orelvis Papayas Virus de las Plantas Plant Viruses Luffa Aegyptica Papaya Ringspot Virus Virus de la Mancha de Anillo de la Papaya Sponge Gourd Cuba Sequencing Secuenciación Luffa aegyptiaca (sponge gourd) plants showing severe leaf mosaic and deformation symptoms were observed in Villa Clara, Cuba. Electron microscopy observations of leaf dip preparations revealed flexuous filamentous particles, which were identified as Papaya ringspot virus (PRSV) by ELISA test. PRSV was mechanically transmitted to healthy Cucurbita moschata plants. Sequence analysis of the RT-PCR product obtained from the capsid protein gene showed highest identity with other PRSV isolates from United States of America, Australia, Venezuela, Brazil, India and Cuba, ranging between 93.2–96.5% and 93.4–98.1% for nucleotide and amino acid sequences, respectively. Phylogenetic analysis showed two main clusters. Cluster I included isolates from the Americas-Australia group and India, including sponge gourd isolate, while the cluster II included isolates from China, Thailand and Taiwan. To our knowledge, this is the first report of PRSV-W infecting sponge gourd in Cuba. Instituto de Patología Vegetal Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias. Departamento de Agronomía; Cuba Fil. Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Portal, Orelvis. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias. Departamento de Biología; Cuba 2018-10-19T14:57:42Z 2018-10-19T14:57:42Z 2017-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3647 https://link.springer.com/article/10.1007%2Fs13314-017-0229-6#citeas 1833-928X (Online) https://doi.org/10.1007/s13314-017-0229-6 eng info:eu-repo/semantics/restrictedAccess application/pdf Cuba (nation) Springer Australasian plant disease notes 12 : 5. (December 2017) |
| spellingShingle | Papayas Virus de las Plantas Plant Viruses Luffa Aegyptica Papaya Ringspot Virus Virus de la Mancha de Anillo de la Papaya Sponge Gourd Cuba Sequencing Secuenciación Cabrera Mederos, Dariel Giolitti, Fabian Nome Docampo, Claudia Bejerman, Nicolas Esteban Portal, Orelvis Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba |
| title | Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba |
| title_full | Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba |
| title_fullStr | Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba |
| title_full_unstemmed | Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba |
| title_short | Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba |
| title_sort | papaya ringspot virus w infecting luffa aegyptiaca in cuba |
| topic | Papayas Virus de las Plantas Plant Viruses Luffa Aegyptica Papaya Ringspot Virus Virus de la Mancha de Anillo de la Papaya Sponge Gourd Cuba Sequencing Secuenciación |
| url | http://hdl.handle.net/20.500.12123/3647 https://link.springer.com/article/10.1007%2Fs13314-017-0229-6#citeas https://doi.org/10.1007/s13314-017-0229-6 |
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