Identification and characterization of a new potyvirus infecting cucurbits
A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813...
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2018
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| Online Access: | http://hdl.handle.net/20.500.12123/3632 https://link.springer.com/article/10.1007%2Fs00705-017-3660-2#citeas https://doi.org/10.1007/s00705-017-3660-2 |
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| author | Perotto, Maria Cecilia Pozzi, Elizabeth Alicia Celli, Marcos Giovani Luciani, Cecilia Elizabeth Mitidieri, Mariel Silvina Conci, Vilma Cecilia |
| author_browse | Celli, Marcos Giovani Conci, Vilma Cecilia Luciani, Cecilia Elizabeth Mitidieri, Mariel Silvina Perotto, Maria Cecilia Pozzi, Elizabeth Alicia |
| author_facet | Perotto, Maria Cecilia Pozzi, Elizabeth Alicia Celli, Marcos Giovani Luciani, Cecilia Elizabeth Mitidieri, Mariel Silvina Conci, Vilma Cecilia |
| author_sort | Perotto, Maria Cecilia |
| collection | INTA Digital |
| description | A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus. |
| format | Artículo |
| id | INTA3632 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA36322022-11-09T11:29:40Z Identification and characterization of a new potyvirus infecting cucurbits Perotto, Maria Cecilia Pozzi, Elizabeth Alicia Celli, Marcos Giovani Luciani, Cecilia Elizabeth Mitidieri, Mariel Silvina Conci, Vilma Cecilia Potyviruses Identification Plant Viruses Squashes Potyvirus Cucurbitaceae Identificación Virus de las Plantas Calabazas San Pedro, Buenos Aires Illumina Sequencing A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus. Instituto de Patología Vegetal Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pozzi, E.A. Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba; Argentina Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba; Argentina Fil: Luciani, Cecilia E. Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba; Argentina Fil: Mitidieri, Mariel Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2018-10-18T15:21:25Z 2018-10-18T15:21:25Z 2018-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3632 https://link.springer.com/article/10.1007%2Fs00705-017-3660-2#citeas 0304-8608 1432-8798 (Online) https://doi.org/10.1007/s00705-017-3660-2 eng info:eu-repo/semantics/restrictedAccess application/pdf San Pedro (inhabited place) Buenos Aires (province) Springer Archives of virology 163 (3) : 719–724. (March 2018) |
| spellingShingle | Potyviruses Identification Plant Viruses Squashes Potyvirus Cucurbitaceae Identificación Virus de las Plantas Calabazas San Pedro, Buenos Aires Illumina Sequencing Perotto, Maria Cecilia Pozzi, Elizabeth Alicia Celli, Marcos Giovani Luciani, Cecilia Elizabeth Mitidieri, Mariel Silvina Conci, Vilma Cecilia Identification and characterization of a new potyvirus infecting cucurbits |
| title | Identification and characterization of a new potyvirus infecting cucurbits |
| title_full | Identification and characterization of a new potyvirus infecting cucurbits |
| title_fullStr | Identification and characterization of a new potyvirus infecting cucurbits |
| title_full_unstemmed | Identification and characterization of a new potyvirus infecting cucurbits |
| title_short | Identification and characterization of a new potyvirus infecting cucurbits |
| title_sort | identification and characterization of a new potyvirus infecting cucurbits |
| topic | Potyviruses Identification Plant Viruses Squashes Potyvirus Cucurbitaceae Identificación Virus de las Plantas Calabazas San Pedro, Buenos Aires Illumina Sequencing |
| url | http://hdl.handle.net/20.500.12123/3632 https://link.springer.com/article/10.1007%2Fs00705-017-3660-2#citeas https://doi.org/10.1007/s00705-017-3660-2 |
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