Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina

In Argentina, more than 60 % of the tobacco crops are grown in the northwestern part of the country and where Rhizoctonia solani leads to a reduction in crop yield and quality. In this study, 35 isolates of Rhizoctonia were obtained from 32 tobacco fields in northwestern Argentina and characterized...

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Main Authors: Mercado Cardenas, Guadalupe Eugenia, Galvan, Marta Zulema, Barrera, Viviana Andrea, Rodriguero, Marcela Silvina, Carmona, Marcelo Anibal, March, Guillermo Juan, Ramallo, Ana Carolina, Shew, H. David
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: 2018
Subjects:
Online Access:https://link.springer.com/article/10.1007/s40858-015-0035-7
http://hdl.handle.net/20.500.12123/3622
https://doi.org/10.1007/s40858-015-0035-7
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author Mercado Cardenas, Guadalupe Eugenia
Galvan, Marta Zulema
Barrera, Viviana Andrea
Rodriguero, Marcela Silvina
Carmona, Marcelo Anibal
March, Guillermo Juan
Ramallo, Ana Carolina
Shew, H. David
author_browse Barrera, Viviana Andrea
Carmona, Marcelo Anibal
Galvan, Marta Zulema
March, Guillermo Juan
Mercado Cardenas, Guadalupe Eugenia
Ramallo, Ana Carolina
Rodriguero, Marcela Silvina
Shew, H. David
author_facet Mercado Cardenas, Guadalupe Eugenia
Galvan, Marta Zulema
Barrera, Viviana Andrea
Rodriguero, Marcela Silvina
Carmona, Marcelo Anibal
March, Guillermo Juan
Ramallo, Ana Carolina
Shew, H. David
author_sort Mercado Cardenas, Guadalupe Eugenia
collection INTA Digital
description In Argentina, more than 60 % of the tobacco crops are grown in the northwestern part of the country and where Rhizoctonia solani leads to a reduction in crop yield and quality. In this study, 35 isolates of Rhizoctonia were obtained from 32 tobacco fields in northwestern Argentina and characterized by both morphological and molecular approaches. Based on the variability in the ITS region, isolates were identified as R. solani (80 %), Waitea circinata var. zeae (Rhizoctonia zeae) (8 %) and binucleate Rhizoctonia (8 %). Most isolates of R. solani belonged to the anastomosis groups (AGs) AG 4 HG-I (44 %), AG 2-1 (41 %) and AG 4 HG-III (13 %). Isolates of binucleate Rhizoctonia belonged to AG-F and AG-P of Ceratobasidium sp. Morphological variability was higher within isolates of AG 2-1 and AG 4 HG-III than within those of AG 4 HG-I. Aggressiveness of the isolates towards tobacco seedlings was assessed in the greenhouse. Isolates of AG 2-1 were the most aggressive on leaves, causing target spot, whereas isolates of AG 4 HG-I were the most aggressive on stems and roots, causing damping-off.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA36222022-05-31T12:03:47Z Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina Mercado Cardenas, Guadalupe Eugenia Galvan, Marta Zulema Barrera, Viviana Andrea Rodriguero, Marcela Silvina Carmona, Marcelo Anibal March, Guillermo Juan Ramallo, Ana Carolina Shew, H. David Rhizoctonia solani Nicotiana tabacum Tabaco Enfermedades de las Plantas Rendimiento Calidad Tobacco Plant Diseases Yields Quality Región Noroeste, Argentina In Argentina, more than 60 % of the tobacco crops are grown in the northwestern part of the country and where Rhizoctonia solani leads to a reduction in crop yield and quality. In this study, 35 isolates of Rhizoctonia were obtained from 32 tobacco fields in northwestern Argentina and characterized by both morphological and molecular approaches. Based on the variability in the ITS region, isolates were identified as R. solani (80 %), Waitea circinata var. zeae (Rhizoctonia zeae) (8 %) and binucleate Rhizoctonia (8 %). Most isolates of R. solani belonged to the anastomosis groups (AGs) AG 4 HG-I (44 %), AG 2-1 (41 %) and AG 4 HG-III (13 %). Isolates of binucleate Rhizoctonia belonged to AG-F and AG-P of Ceratobasidium sp. Morphological variability was higher within isolates of AG 2-1 and AG 4 HG-III than within those of AG 4 HG-I. Aggressiveness of the isolates towards tobacco seedlings was assessed in the greenhouse. Isolates of AG 2-1 were the most aggressive on leaves, causing target spot, whereas isolates of AG 4 HG-I were the most aggressive on stems and roots, causing damping-off. EEA Salta Fil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Galvan, Marta Zulema. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Fil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Fil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía; Argentina Fil: March, Guillermo Juan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Ramallo, Ana C.. Universidad Nacional de Tucumán; Argentina Fil: Shew, H. David. University of North Carolina. Department of Plant Pathology; Estados Unidos 2018-10-18T12:38:37Z 2018-10-18T12:38:37Z 2015-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007/s40858-015-0035-7 http://hdl.handle.net/20.500.12123/3622 1983-2052 https://doi.org/10.1007/s40858-015-0035-7 eng info:eu-repo/semantics/restrictedAccess application/pdf Tropical Plant Pathology 40 (3) : 160–168 (June 2015)
spellingShingle Rhizoctonia solani
Nicotiana tabacum
Tabaco
Enfermedades de las Plantas
Rendimiento
Calidad
Tobacco
Plant Diseases
Yields
Quality
Región Noroeste, Argentina
Mercado Cardenas, Guadalupe Eugenia
Galvan, Marta Zulema
Barrera, Viviana Andrea
Rodriguero, Marcela Silvina
Carmona, Marcelo Anibal
March, Guillermo Juan
Ramallo, Ana Carolina
Shew, H. David
Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina
title Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina
title_full Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina
title_fullStr Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina
title_full_unstemmed Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina
title_short Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina
title_sort molecular identification and pathogenicity of rhizoctonia spp from tobacco growing areas in northwestern argentina
topic Rhizoctonia solani
Nicotiana tabacum
Tabaco
Enfermedades de las Plantas
Rendimiento
Calidad
Tobacco
Plant Diseases
Yields
Quality
Región Noroeste, Argentina
url https://link.springer.com/article/10.1007/s40858-015-0035-7
http://hdl.handle.net/20.500.12123/3622
https://doi.org/10.1007/s40858-015-0035-7
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