Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina
Common bean web blight (WB), caused by the fungus Rhizoctonia solani (teleomorph Thanatephorus cucumeris), is among the endemic fungal diseases of major impact in north‐western Argentina (NWA). This study aimed to analyse the genetic and pathogenic diversity of R. solani in Salta, NWA, where 97 isol...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | http://hdl.handle.net/20.500.12123/3619 https://onlinelibrary.wiley.com/doi/full/10.1111/jph.12526 https://doi.org/10.1111/jph.12526 |
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| author | Spedaletti, Yamila Andrea Aparicio Gonzalez, Monica Alicia Mercado Cardenas, Guadalupe Eugenia Rodriguero, Marcela Silvina Taboada, Gisel María Aban, Carla Luciana Sühring, Silvia Susana Vizgarra, Oscar Nicéforo Galvan, Marta Zulema |
| author_browse | Aban, Carla Luciana Aparicio Gonzalez, Monica Alicia Galvan, Marta Zulema Mercado Cardenas, Guadalupe Eugenia Rodriguero, Marcela Silvina Spedaletti, Yamila Andrea Sühring, Silvia Susana Taboada, Gisel María Vizgarra, Oscar Nicéforo |
| author_facet | Spedaletti, Yamila Andrea Aparicio Gonzalez, Monica Alicia Mercado Cardenas, Guadalupe Eugenia Rodriguero, Marcela Silvina Taboada, Gisel María Aban, Carla Luciana Sühring, Silvia Susana Vizgarra, Oscar Nicéforo Galvan, Marta Zulema |
| author_sort | Spedaletti, Yamila Andrea |
| collection | INTA Digital |
| description | Common bean web blight (WB), caused by the fungus Rhizoctonia solani (teleomorph Thanatephorus cucumeris), is among the endemic fungal diseases of major impact in north‐western Argentina (NWA). This study aimed to analyse the genetic and pathogenic diversity of R. solani in Salta, NWA, where 97 isolates were recovered from commercial bean cultivars and wild beans showing WB symptoms in a major bean production area. The isolates were characterized on the basis of specific primers, rDNA‐ITS sequences and morphological characteristics. All the isolates were identified as R. solani AG 2‐2WB, and they exhibited considerable intragroup variation. The phylogenetic tree generated with the ITS sequences confirmed the isolates identification. Aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. A great variability in virulence was observed among the isolates analysed. On the basis of the disease reaction on foliar tissues, the isolates were grouped into three virulence categories as follows: weakly virulent (30%), moderately virulent (38%) and highly virulent (32%). However, no correlation between virulence and geographical origin was detected. The information generated in this study provides initial data on the population variability of the WB pathogen in north‐western Argentina and represents a valuable contribution to regional breeding programmes aimed to obtain cultivars with durable resistance. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3619 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA36192025-07-30T12:05:33Z Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina Spedaletti, Yamila Andrea Aparicio Gonzalez, Monica Alicia Mercado Cardenas, Guadalupe Eugenia Rodriguero, Marcela Silvina Taboada, Gisel María Aban, Carla Luciana Sühring, Silvia Susana Vizgarra, Oscar Nicéforo Galvan, Marta Zulema Phaseolus vulgaris Fríjol (phaseolus) Rhizoctonia solani Enfermedades de las Plantas Patogenicidad Genética Podredumbre de la Raíz Kidney Beans Plant Diseases Pathogenicity Genetics Root Rots Poroto Argentina Common bean web blight (WB), caused by the fungus Rhizoctonia solani (teleomorph Thanatephorus cucumeris), is among the endemic fungal diseases of major impact in north‐western Argentina (NWA). This study aimed to analyse the genetic and pathogenic diversity of R. solani in Salta, NWA, where 97 isolates were recovered from commercial bean cultivars and wild beans showing WB symptoms in a major bean production area. The isolates were characterized on the basis of specific primers, rDNA‐ITS sequences and morphological characteristics. All the isolates were identified as R. solani AG 2‐2WB, and they exhibited considerable intragroup variation. The phylogenetic tree generated with the ITS sequences confirmed the isolates identification. Aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. A great variability in virulence was observed among the isolates analysed. On the basis of the disease reaction on foliar tissues, the isolates were grouped into three virulence categories as follows: weakly virulent (30%), moderately virulent (38%) and highly virulent (32%). However, no correlation between virulence and geographical origin was detected. The information generated in this study provides initial data on the population variability of the WB pathogen in north‐western Argentina and represents a valuable contribution to regional breeding programmes aimed to obtain cultivars with durable resistance. EEA Salta Fil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Aparicio Gonzalez, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Fil: Taboada, Gisel María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Aban, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Sühring, Silvia Susana. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina Fil: Vizgarra, Oscar N. Estación Experimental Agroindustrial Obispo Colombres; Argentina Fil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina 2018-10-17T17:44:17Z 2018-10-17T17:44:17Z 2016-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3619 https://onlinelibrary.wiley.com/doi/full/10.1111/jph.12526 0931-1785 1439-0434 https://doi.org/10.1111/jph.12526 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of Phytopathology 164 (11-12) : 1054-1063 (December 2016) |
| spellingShingle | Phaseolus vulgaris Fríjol (phaseolus) Rhizoctonia solani Enfermedades de las Plantas Patogenicidad Genética Podredumbre de la Raíz Kidney Beans Plant Diseases Pathogenicity Genetics Root Rots Poroto Argentina Spedaletti, Yamila Andrea Aparicio Gonzalez, Monica Alicia Mercado Cardenas, Guadalupe Eugenia Rodriguero, Marcela Silvina Taboada, Gisel María Aban, Carla Luciana Sühring, Silvia Susana Vizgarra, Oscar Nicéforo Galvan, Marta Zulema Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina |
| title | Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina |
| title_full | Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina |
| title_fullStr | Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina |
| title_full_unstemmed | Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina |
| title_short | Genetic characterization and pathogenicity of Rhizoctonia solani associated with common bean web blight in the main bean growing area of Argentina |
| title_sort | genetic characterization and pathogenicity of rhizoctonia solani associated with common bean web blight in the main bean growing area of argentina |
| topic | Phaseolus vulgaris Fríjol (phaseolus) Rhizoctonia solani Enfermedades de las Plantas Patogenicidad Genética Podredumbre de la Raíz Kidney Beans Plant Diseases Pathogenicity Genetics Root Rots Poroto Argentina |
| url | http://hdl.handle.net/20.500.12123/3619 https://onlinelibrary.wiley.com/doi/full/10.1111/jph.12526 https://doi.org/10.1111/jph.12526 |
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