Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies
Species of Cedrela with a high economic value from Northwest and Northeastern Argentina are severely exploited. This work evaluates whether 51 nuclear SSRs, developed to study phylogenetically close species in the Meliaceae family (Cedrela odorata, Cedrela fissilis, Swietenia humilis and Swietenia m...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/3617 https://www.sciencedirect.com/science/article/pii/S0305197813002366?via%3Dihub https://doi.org/10.1016/j.bse.2013.12.003 |
| _version_ | 1855483271908950016 |
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| author | Soldati, Maria Cristina Inza, Maria Virginia Fornes, Luis Fernando Zelener, Noga |
| author_browse | Fornes, Luis Fernando Inza, Maria Virginia Soldati, Maria Cristina Zelener, Noga |
| author_facet | Soldati, Maria Cristina Inza, Maria Virginia Fornes, Luis Fernando Zelener, Noga |
| author_sort | Soldati, Maria Cristina |
| collection | INTA Digital |
| description | Species of Cedrela with a high economic value from Northwest and Northeastern Argentina are severely exploited. This work evaluates whether 51 nuclear SSRs, developed to study phylogenetically close species in the Meliaceae family (Cedrela odorata, Cedrela fissilis, Swietenia humilis and Swietenia macrophylla), can be used to study C. fissilis, Cedrela balansae, Cedrela saltensis and Cedrela angustifolia. A 62.8% of the total of 194 SSRs/species combinations showed a successful, homologous and cross-species amplification. As expected, a great success in SSRs transferability among Cedrela species was observed. Twenty-one screened SSRs showed a successful amplification pattern in all target species and many of them were polymorphic (9, 13, 13 and 7 SSRs for C. fissilis, C. balansae, C. saltensis and C. angustifolia, respectively). The high number of evaluated SSRs from the Cedrela genus and Meliaceae family, allowed us to obtain a suitable set of validated markers that are highly variable and easily scored, and also identify those which were less sturdy. We were able to retain a useful set of markers for three of the target species, but not for C. angustifolia. This could be due to its greater phylogenetic and morphological distances to the other three species. The lack of SSRs developed for our target species, transforms the transferred SSRs reported here in a valuable tool to monitor the genetic consequences of forest overexploitation on Cedrela species |
| format | Artículo |
| id | INTA3617 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA36172018-10-17T16:38:13Z Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies Soldati, Maria Cristina Inza, Maria Virginia Fornes, Luis Fernando Zelener, Noga Cedrela Genética de Poblaciones Especies en Peligro de Extinción Meliaceae Microsatélites Population Genetics Endangered Species Microsatellites Transferability Cross-species Transferibilidad Species of Cedrela with a high economic value from Northwest and Northeastern Argentina are severely exploited. This work evaluates whether 51 nuclear SSRs, developed to study phylogenetically close species in the Meliaceae family (Cedrela odorata, Cedrela fissilis, Swietenia humilis and Swietenia macrophylla), can be used to study C. fissilis, Cedrela balansae, Cedrela saltensis and Cedrela angustifolia. A 62.8% of the total of 194 SSRs/species combinations showed a successful, homologous and cross-species amplification. As expected, a great success in SSRs transferability among Cedrela species was observed. Twenty-one screened SSRs showed a successful amplification pattern in all target species and many of them were polymorphic (9, 13, 13 and 7 SSRs for C. fissilis, C. balansae, C. saltensis and C. angustifolia, respectively). The high number of evaluated SSRs from the Cedrela genus and Meliaceae family, allowed us to obtain a suitable set of validated markers that are highly variable and easily scored, and also identify those which were less sturdy. We were able to retain a useful set of markers for three of the target species, but not for C. angustifolia. This could be due to its greater phylogenetic and morphological distances to the other three species. The lack of SSRs developed for our target species, transforms the transferred SSRs reported here in a valuable tool to monitor the genetic consequences of forest overexploitation on Cedrela species Instituto de Recursos Biológicos Fil: Soldati, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Inza, Maria Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Fornes, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Zelener, Noga. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina 2018-10-17T16:32:52Z 2018-10-17T16:32:52Z 2014-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3617 https://www.sciencedirect.com/science/article/pii/S0305197813002366?via%3Dihub 0305-1978 https://doi.org/10.1016/j.bse.2013.12.003 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Biochemical systematics and ecology 53 : 8-16. (April 2014) |
| spellingShingle | Cedrela Genética de Poblaciones Especies en Peligro de Extinción Meliaceae Microsatélites Population Genetics Endangered Species Microsatellites Transferability Cross-species Transferibilidad Soldati, Maria Cristina Inza, Maria Virginia Fornes, Luis Fernando Zelener, Noga Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies |
| title | Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies |
| title_full | Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies |
| title_fullStr | Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies |
| title_full_unstemmed | Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies |
| title_short | Cross transferability of SSR markers to endangered Cedrela species that grow in Argentinean subtropical forests, as a valuable tool for population genetic studies |
| title_sort | cross transferability of ssr markers to endangered cedrela species that grow in argentinean subtropical forests as a valuable tool for population genetic studies |
| topic | Cedrela Genética de Poblaciones Especies en Peligro de Extinción Meliaceae Microsatélites Population Genetics Endangered Species Microsatellites Transferability Cross-species Transferibilidad |
| url | http://hdl.handle.net/20.500.12123/3617 https://www.sciencedirect.com/science/article/pii/S0305197813002366?via%3Dihub https://doi.org/10.1016/j.bse.2013.12.003 |
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