Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina
The objective of this study was to perform the molecular and pathogenic characterization of Rhizoctonia solani in bean seed and soil samples from fields with root rot symptoms in northwestern Argentina. Fifty-two Rhizoctonia spp. isolates were collected in ten naturally infested bean fields and char...
| Autores principales: | , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/3609 https://doi.org/10.21475/ajcs.17.11.08.pne432 |
| _version_ | 1855035172196450304 |
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| author | Spedaletti, Yamila Andrea Mercado Cardenas, Guadalupe Eugenia Taboada, Gisel María Aban, Carla Luciana Aparicio Gonzalez, Monica Alicia Rodriguero, Marcela Silvina Vizgarra, Oscar Nicéforo Sühring, Silvia Susana Galindez, Guadalupe Galvan, Marta Zulema |
| author_browse | Aban, Carla Luciana Aparicio Gonzalez, Monica Alicia Galindez, Guadalupe Galvan, Marta Zulema Mercado Cardenas, Guadalupe Eugenia Rodriguero, Marcela Silvina Spedaletti, Yamila Andrea Sühring, Silvia Susana Taboada, Gisel María Vizgarra, Oscar Nicéforo |
| author_facet | Spedaletti, Yamila Andrea Mercado Cardenas, Guadalupe Eugenia Taboada, Gisel María Aban, Carla Luciana Aparicio Gonzalez, Monica Alicia Rodriguero, Marcela Silvina Vizgarra, Oscar Nicéforo Sühring, Silvia Susana Galindez, Guadalupe Galvan, Marta Zulema |
| author_sort | Spedaletti, Yamila Andrea |
| collection | INTA Digital |
| description | The objective of this study was to perform the molecular and pathogenic characterization of Rhizoctonia solani in bean seed and soil samples from fields with root rot symptoms in northwestern Argentina. Fifty-two Rhizoctonia spp. isolates were collected in ten naturally infested bean fields and characterized by morphological traits, DNA sequencing, and pathogenicity. The mycelium color, the pattern of sclerotia formation, and the number of nuclei per hyphal cell were determined for each isolate. According to the variability in the rDNA-ITS region, isolates were identified as R. solani (85%), Waitea circinata var. zeae (Rhizoctonia zeae) (2%) and Rhizoctonia spp. (13%). Most isolates of R. solani (92%) were found to belong to the anastomosis group (AG) AG 4, including seven AG 4 HG-I and nine AG 4 HG-III isolates. AGs obtained from soil samples were more variable than those obtained from seed samples. Molecular identification of the isolates was in agreement with their morphological characterization. In addition, aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. Four virulence categories were defined according to the disease reaction on root and foliar tissues, which showed great variability in virulence among the isolates. Our results suggest that both seed and soil-borne inoculum may play a significant role in pathogen dispersal in the region. This is the first study on Rhizoctonia species and AGs in bean seed and soil in this region and it may contribute towards an efficient control strategy for bean diseases caused by Rhizoctonia species. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3609 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA36092025-07-30T12:05:40Z Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina Spedaletti, Yamila Andrea Mercado Cardenas, Guadalupe Eugenia Taboada, Gisel María Aban, Carla Luciana Aparicio Gonzalez, Monica Alicia Rodriguero, Marcela Silvina Vizgarra, Oscar Nicéforo Sühring, Silvia Susana Galindez, Guadalupe Galvan, Marta Zulema Rhizoctonia solani Phaseolus vulgaris Fríjol (phaseolus) Enfermedades de las Plantas Patogenicidad Podredumbre de la Raíz Kidney Beans Plant Diseases Pathogenicity Root Rots Soil Suelo Argentina Poroto The objective of this study was to perform the molecular and pathogenic characterization of Rhizoctonia solani in bean seed and soil samples from fields with root rot symptoms in northwestern Argentina. Fifty-two Rhizoctonia spp. isolates were collected in ten naturally infested bean fields and characterized by morphological traits, DNA sequencing, and pathogenicity. The mycelium color, the pattern of sclerotia formation, and the number of nuclei per hyphal cell were determined for each isolate. According to the variability in the rDNA-ITS region, isolates were identified as R. solani (85%), Waitea circinata var. zeae (Rhizoctonia zeae) (2%) and Rhizoctonia spp. (13%). Most isolates of R. solani (92%) were found to belong to the anastomosis group (AG) AG 4, including seven AG 4 HG-I and nine AG 4 HG-III isolates. AGs obtained from soil samples were more variable than those obtained from seed samples. Molecular identification of the isolates was in agreement with their morphological characterization. In addition, aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. Four virulence categories were defined according to the disease reaction on root and foliar tissues, which showed great variability in virulence among the isolates. Our results suggest that both seed and soil-borne inoculum may play a significant role in pathogen dispersal in the region. This is the first study on Rhizoctonia species and AGs in bean seed and soil in this region and it may contribute towards an efficient control strategy for bean diseases caused by Rhizoctonia species. EEA Salta Fil: Spedaletti, Yamila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Mercado Cardenas, Guadalupe Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Taboada, Gisel María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Aban, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Aparicio Gonzalez, Monica Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Rodriguero, Marcela Silvina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Fil: Vizgarra, Oscar N. Estación Experimental Agroindustrial Obispo Colombres; Argentina Fil: Sühring, Silvia Susana. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina Fil: Galindez, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Fil: Galvan, Marta Zulema. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina 2018-10-17T13:29:46Z 2018-10-17T13:29:46Z 2017-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3609 1835-2693 1835-2707 https://doi.org/10.21475/ajcs.17.11.08.pne432 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Australian Journal of Crop Science 11 (8) : 952-959 (August 2017) |
| spellingShingle | Rhizoctonia solani Phaseolus vulgaris Fríjol (phaseolus) Enfermedades de las Plantas Patogenicidad Podredumbre de la Raíz Kidney Beans Plant Diseases Pathogenicity Root Rots Soil Suelo Argentina Poroto Spedaletti, Yamila Andrea Mercado Cardenas, Guadalupe Eugenia Taboada, Gisel María Aban, Carla Luciana Aparicio Gonzalez, Monica Alicia Rodriguero, Marcela Silvina Vizgarra, Oscar Nicéforo Sühring, Silvia Susana Galindez, Guadalupe Galvan, Marta Zulema Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina |
| title | Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina |
| title_full | Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina |
| title_fullStr | Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina |
| title_full_unstemmed | Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina |
| title_short | Molecular identification and pathogenicity of 'Rhizoctonia' spp. recovered from seed and soil samples of the main bean growing area of Argentina |
| title_sort | molecular identification and pathogenicity of rhizoctonia spp recovered from seed and soil samples of the main bean growing area of argentina |
| topic | Rhizoctonia solani Phaseolus vulgaris Fríjol (phaseolus) Enfermedades de las Plantas Patogenicidad Podredumbre de la Raíz Kidney Beans Plant Diseases Pathogenicity Root Rots Soil Suelo Argentina Poroto |
| url | http://hdl.handle.net/20.500.12123/3609 https://doi.org/10.21475/ajcs.17.11.08.pne432 |
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