Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells
We used transient expression assays to assess the function of the baculovirus Spodoptera frugiperda M nucleopolyhedrovirus (SfMNPV) homologs of Autographa californica MNPV (AcMNPV) factors involved in late gene expression (lefs), in the Sf9 insect cell-line, which is permissive for both viruses. It...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2018
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| Acceso en línea: | https://link.springer.com/article/10.1007%2Fs11262-012-0843-5 http://hdl.handle.net/20.500.12123/3598 https://doi.org/10.1007/s11262-012-0843-5 |
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| author | Berretta, Marcelo Facundo Lopez, Maria Gabriela Taboga, Oscar Alberto Sciocco, Alicia Ines Romanowski, Victor |
| author_browse | Berretta, Marcelo Facundo Lopez, Maria Gabriela Romanowski, Victor Sciocco, Alicia Ines Taboga, Oscar Alberto |
| author_facet | Berretta, Marcelo Facundo Lopez, Maria Gabriela Taboga, Oscar Alberto Sciocco, Alicia Ines Romanowski, Victor |
| author_sort | Berretta, Marcelo Facundo |
| collection | INTA Digital |
| description | We used transient expression assays to assess the function of the baculovirus Spodoptera frugiperda M nucleopolyhedrovirus (SfMNPV) homologs of Autographa californica MNPV (AcMNPV) factors involved in late gene expression (lefs), in the Sf9 insect cell-line, which is permissive for both viruses. It is well-established that nineteen AcMNPV lefs support optimal levels of activity from a late promoter-reporter gene cassette in this assay. A subgroup of SfMNPV lefs predicted to function in transcription-specific events substituted the corresponding AcMNPV lefs very efficiently. When all SfMNPV lefs were assayed, including replication lefs, activity was low, but addition of two AcMNPV lefs not encoded in SfMNPV genome, resulted in augmented reporter activity. SfMNPV IE-1 was able to activate an early promoter cis-linked to an hr-derived element from SfMNPV but not from AcMNPV. However, the level of early promoter activation with SfMNPV IE-1 was lower compared to AcMNPV IE-1. |
| format | Artículo |
| id | INTA3598 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA35982018-10-16T12:56:48Z Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells Berretta, Marcelo Facundo Lopez, Maria Gabriela Taboga, Oscar Alberto Sciocco, Alicia Ines Romanowski, Victor Spodoptera frugiperda Lepidoptera Expresión Génica Baculovirus Gene Expression Nucleopolyhedrovirus Sf9 Cells We used transient expression assays to assess the function of the baculovirus Spodoptera frugiperda M nucleopolyhedrovirus (SfMNPV) homologs of Autographa californica MNPV (AcMNPV) factors involved in late gene expression (lefs), in the Sf9 insect cell-line, which is permissive for both viruses. It is well-established that nineteen AcMNPV lefs support optimal levels of activity from a late promoter-reporter gene cassette in this assay. A subgroup of SfMNPV lefs predicted to function in transcription-specific events substituted the corresponding AcMNPV lefs very efficiently. When all SfMNPV lefs were assayed, including replication lefs, activity was low, but addition of two AcMNPV lefs not encoded in SfMNPV genome, resulted in augmented reporter activity. SfMNPV IE-1 was able to activate an early promoter cis-linked to an hr-derived element from SfMNPV but not from AcMNPV. However, the level of early promoter activation with SfMNPV IE-1 was lower compared to AcMNPV IE-1. Instituto de Microbiología y Zoología Agrícola Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Romanowski, Victor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina 2018-10-16T12:55:12Z 2018-10-16T12:55:12Z 2013-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://link.springer.com/article/10.1007%2Fs11262-012-0843-5 http://hdl.handle.net/20.500.12123/3598 0920-8569 1572-994X https://doi.org/10.1007/s11262-012-0843-5 eng info:eu-repo/semantics/restrictedAccess application/pdf Virus Genes 46 (1) : 152–161 (February 2013) |
| spellingShingle | Spodoptera frugiperda Lepidoptera Expresión Génica Baculovirus Gene Expression Nucleopolyhedrovirus Sf9 Cells Berretta, Marcelo Facundo Lopez, Maria Gabriela Taboga, Oscar Alberto Sciocco, Alicia Ines Romanowski, Victor Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells |
| title | Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells |
| title_full | Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells |
| title_fullStr | Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells |
| title_full_unstemmed | Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells |
| title_short | Functional analysis of Spodoptera frugiperda nucleopolyhedrovirus late expression factors in Sf9 cells |
| title_sort | functional analysis of spodoptera frugiperda nucleopolyhedrovirus late expression factors in sf9 cells |
| topic | Spodoptera frugiperda Lepidoptera Expresión Génica Baculovirus Gene Expression Nucleopolyhedrovirus Sf9 Cells |
| url | https://link.springer.com/article/10.1007%2Fs11262-012-0843-5 http://hdl.handle.net/20.500.12123/3598 https://doi.org/10.1007/s11262-012-0843-5 |
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