Evaluation of total white blood cell count as a marker for proviral load of bovine leukemia virus in dairy cattle from herds with a high seroprevalence of antibodies against bovine leukemia virus
Objective—To determine the reference interval for WBC counts in Holstein dairy cows from herds with high seroprevalence for anti–bovine leukemia virus (BLV) antibodies, analyze the correlation of total WBC counts and blood proviral load (bPVL) in BLV-infected animals, and determine whether total WBC...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2018
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| Online Access: | https://avmajournals.avma.org/doi/abs/10.2460/ajvr.74.5.744?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed http://hdl.handle.net/20.500.12123/3584 https://doi.org/10.2460/ajvr.74.5.744 |
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| author | Alvarez, Irene Gutierrez, Gerónimo Gammella, Mariela Martínez, Cecilia Politzki, Romina Paula González, Cintia Beatríz Caviglia, Luciana Carignano, Hugo Fondevila, Norberto Antonio Poli, Mario Andres Trono, Karina Gabriela |
| author_browse | Alvarez, Irene Carignano, Hugo Caviglia, Luciana Fondevila, Norberto Antonio Gammella, Mariela González, Cintia Beatríz Gutierrez, Gerónimo Martínez, Cecilia Poli, Mario Andres Politzki, Romina Paula Trono, Karina Gabriela |
| author_facet | Alvarez, Irene Gutierrez, Gerónimo Gammella, Mariela Martínez, Cecilia Politzki, Romina Paula González, Cintia Beatríz Caviglia, Luciana Carignano, Hugo Fondevila, Norberto Antonio Poli, Mario Andres Trono, Karina Gabriela |
| author_sort | Alvarez, Irene |
| collection | INTA Digital |
| description | Objective—To determine the reference interval for WBC counts in Holstein dairy cows from herds with high seroprevalence for anti–bovine leukemia virus (BLV) antibodies, analyze the correlation of total WBC counts and blood proviral load (bPVL) in BLV-infected animals, and determine whether total WBC count can be used a hematologic marker for in vivo infection.
Animals—307 lactating cows from 16 dairy herds with high BLV seroprevalence.
Procedures—Blood samples were collected for assessment of plasma anti–BLV p24 antibody concentration (all cows), manual determination of WBC count (161 BLV-seronegative cows from 15 herds), and evaluation of bPVL (146 cows from another herd).
Results—The WBC count reference interval (ie, mean ± 2 SD) for BLV-seronegative dairy cows was 2,153 to 11,493 cells/μL. Of the 146 cows used to analyze the correlation between WBC count and bPVL, 107 (73%) had WBC counts within the reference interval; of those cows, only 21 (19.6%) had high bPVL. Most cows with high WBC counts (35/39) had high bPVL. Mean WBC count for cows with high bPVL was significantly higher than values for cows with low or undetectable bPVL. White blood cell counts and bPVL were significantly (ρ = 0.71) correlated.
Conclusions and Clinical Relevance—These data have provided an updated reference interval for WBC counts in Holstein cows from herds with high BLV seroprevalence. In dairy cattle under natural conditions, WBC count was correlated with bPVL; thus, WBC count determination could be a potential tool for monitoring BLV infection levels in attempts to control transmission. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3584 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA35842018-10-11T18:56:25Z Evaluation of total white blood cell count as a marker for proviral load of bovine leukemia virus in dairy cattle from herds with a high seroprevalence of antibodies against bovine leukemia virus Alvarez, Irene Gutierrez, Gerónimo Gammella, Mariela Martínez, Cecilia Politzki, Romina Paula González, Cintia Beatríz Caviglia, Luciana Carignano, Hugo Fondevila, Norberto Antonio Poli, Mario Andres Trono, Karina Gabriela Bovine Leukaemia Virus Dairy Cows Antibodies Proviruses Leukocytes Virus Leucemia Bovina Vacas Lecheras Anticuerpos Provirus Leucocitos Objective—To determine the reference interval for WBC counts in Holstein dairy cows from herds with high seroprevalence for anti–bovine leukemia virus (BLV) antibodies, analyze the correlation of total WBC counts and blood proviral load (bPVL) in BLV-infected animals, and determine whether total WBC count can be used a hematologic marker for in vivo infection. Animals—307 lactating cows from 16 dairy herds with high BLV seroprevalence. Procedures—Blood samples were collected for assessment of plasma anti–BLV p24 antibody concentration (all cows), manual determination of WBC count (161 BLV-seronegative cows from 15 herds), and evaluation of bPVL (146 cows from another herd). Results—The WBC count reference interval (ie, mean ± 2 SD) for BLV-seronegative dairy cows was 2,153 to 11,493 cells/μL. Of the 146 cows used to analyze the correlation between WBC count and bPVL, 107 (73%) had WBC counts within the reference interval; of those cows, only 21 (19.6%) had high bPVL. Most cows with high WBC counts (35/39) had high bPVL. Mean WBC count for cows with high bPVL was significantly higher than values for cows with low or undetectable bPVL. White blood cell counts and bPVL were significantly (ρ = 0.71) correlated. Conclusions and Clinical Relevance—These data have provided an updated reference interval for WBC counts in Holstein cows from herds with high BLV seroprevalence. In dairy cattle under natural conditions, WBC count was correlated with bPVL; thus, WBC count determination could be a potential tool for monitoring BLV infection levels in attempts to control transmission. Instituto de Virología Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Martinez, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Politzki, Romina Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Gonzalez, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Caviglia, Luciana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Fondevila, Norberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina 2018-10-11T18:47:59Z 2018-10-11T18:47:59Z 2013-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://avmajournals.avma.org/doi/abs/10.2460/ajvr.74.5.744?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed http://hdl.handle.net/20.500.12123/3584 0002-9645 https://doi.org/10.2460/ajvr.74.5.744 eng info:eu-repo/semantics/restrictedAccess application/pdf American journal of veterinary research 74 (5) : 744-749. (May 2013) |
| spellingShingle | Bovine Leukaemia Virus Dairy Cows Antibodies Proviruses Leukocytes Virus Leucemia Bovina Vacas Lecheras Anticuerpos Provirus Leucocitos Alvarez, Irene Gutierrez, Gerónimo Gammella, Mariela Martínez, Cecilia Politzki, Romina Paula González, Cintia Beatríz Caviglia, Luciana Carignano, Hugo Fondevila, Norberto Antonio Poli, Mario Andres Trono, Karina Gabriela Evaluation of total white blood cell count as a marker for proviral load of bovine leukemia virus in dairy cattle from herds with a high seroprevalence of antibodies against bovine leukemia virus |
| title | Evaluation of total white blood cell count as a marker for proviral load of bovine leukemia virus in dairy cattle from herds with a high seroprevalence of antibodies against bovine leukemia virus |
| title_full | Evaluation of total white blood cell count as a marker for proviral load of bovine leukemia virus in dairy cattle from herds with a high seroprevalence of antibodies against bovine leukemia virus |
| title_fullStr | Evaluation of total white blood cell count as a marker for proviral load of bovine leukemia virus in dairy cattle from herds with a high seroprevalence of antibodies against bovine leukemia virus |
| title_full_unstemmed | Evaluation of total white blood cell count as a marker for proviral load of bovine leukemia virus in dairy cattle from herds with a high seroprevalence of antibodies against bovine leukemia virus |
| title_short | Evaluation of total white blood cell count as a marker for proviral load of bovine leukemia virus in dairy cattle from herds with a high seroprevalence of antibodies against bovine leukemia virus |
| title_sort | evaluation of total white blood cell count as a marker for proviral load of bovine leukemia virus in dairy cattle from herds with a high seroprevalence of antibodies against bovine leukemia virus |
| topic | Bovine Leukaemia Virus Dairy Cows Antibodies Proviruses Leukocytes Virus Leucemia Bovina Vacas Lecheras Anticuerpos Provirus Leucocitos |
| url | https://avmajournals.avma.org/doi/abs/10.2460/ajvr.74.5.744?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed http://hdl.handle.net/20.500.12123/3584 https://doi.org/10.2460/ajvr.74.5.744 |
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