Comparative histopathological and immunological study of two field strains of chicken anemia virus

Infection of poultry with chicken anemia virus (CAV) is implicated in several field problems in broiler flocks due to the immunosuppression generated and, consequently, the increased susceptibility to secondary infections. Recently, we have reported an increased occurrence of clinical cases caused b...

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Autores principales: Rimondi, Agustina, Pinto, Silvina, Olivera, Valeria Soledad, Dibarbora, Marina, Perez Filgueira, Daniel Mariano, Craig, María Isabel, Pereda, Ariel Julian
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: BioMed Central 2018
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/3582
https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-014-0102-y
https://doi.org/10.1186/s13567-014-0102-y
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author Rimondi, Agustina
Pinto, Silvina
Olivera, Valeria Soledad
Dibarbora, Marina
Perez Filgueira, Daniel Mariano
Craig, María Isabel
Pereda, Ariel Julian
author_browse Craig, María Isabel
Dibarbora, Marina
Olivera, Valeria Soledad
Pereda, Ariel Julian
Perez Filgueira, Daniel Mariano
Pinto, Silvina
Rimondi, Agustina
author_facet Rimondi, Agustina
Pinto, Silvina
Olivera, Valeria Soledad
Dibarbora, Marina
Perez Filgueira, Daniel Mariano
Craig, María Isabel
Pereda, Ariel Julian
author_sort Rimondi, Agustina
collection INTA Digital
description Infection of poultry with chicken anemia virus (CAV) is implicated in several field problems in broiler flocks due to the immunosuppression generated and, consequently, the increased susceptibility to secondary infections. Recently, we have reported an increased occurrence of clinical cases caused by CAV strains distantly related to those commonly used for vaccination. In order to understand the behavior of two Argentinean CAV strains (CAV-10 and CAV-18) in two-week-old chickens, an immune and histopathological study was performed. Neither mortality nor clinical signs were observed in the infected or control groups. Thymus lobes from chickens infected with both CAV viruses were smaller compared to the negative control group. At 14 days post-infection (dpi), only chickens inoculated with CAV-10 show a severe depletion of lymphocytes in the thymus cortex and in follicles from the bursa of Fabricius. Also thymopoiesis disorders, such as reduction in the percentage of total DP (CD4 + CD8α+) thymocytes and alteration in the percentages of DP subpopulations, were more important in animals inoculated with the CAV-10 than the CAV-18 strain. In addition, only animals infected with CAV-10 show a decrease in CD8αβ splenocytes. Altogether our results show that, although both Argentinean CAV strains produce subclinical infections in chickens causing immunosuppression at 14 dpi, they might differ in their in vivo pathogenicity.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA35822018-10-11T18:36:35Z Comparative histopathological and immunological study of two field strains of chicken anemia virus Rimondi, Agustina Pinto, Silvina Olivera, Valeria Soledad Dibarbora, Marina Perez Filgueira, Daniel Mariano Craig, María Isabel Pereda, Ariel Julian Chicken Anemia Virus Chickens Animal Diseases Histocytological Analysis Análisis Histocitológico Histopathology Infection Virus Anemia Pollo Pollo Enfermedades de los Animales Histopatología Infección Subclinical Infection Double Positive Cell Negative Control Group Argentina Infection of poultry with chicken anemia virus (CAV) is implicated in several field problems in broiler flocks due to the immunosuppression generated and, consequently, the increased susceptibility to secondary infections. Recently, we have reported an increased occurrence of clinical cases caused by CAV strains distantly related to those commonly used for vaccination. In order to understand the behavior of two Argentinean CAV strains (CAV-10 and CAV-18) in two-week-old chickens, an immune and histopathological study was performed. Neither mortality nor clinical signs were observed in the infected or control groups. Thymus lobes from chickens infected with both CAV viruses were smaller compared to the negative control group. At 14 days post-infection (dpi), only chickens inoculated with CAV-10 show a severe depletion of lymphocytes in the thymus cortex and in follicles from the bursa of Fabricius. Also thymopoiesis disorders, such as reduction in the percentage of total DP (CD4 + CD8α+) thymocytes and alteration in the percentages of DP subpopulations, were more important in animals inoculated with the CAV-10 than the CAV-18 strain. In addition, only animals infected with CAV-10 show a decrease in CD8αβ splenocytes. Altogether our results show that, although both Argentinean CAV strains produce subclinical infections in chickens causing immunosuppression at 14 dpi, they might differ in their in vivo pathogenicity. Instituto de Virología Fil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Pinto, Silvina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Patología Aviar; Argentina Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina Fil: Dibarbora, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina Fil: Pereda, Ariel Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina 2018-10-11T17:36:56Z 2018-10-11T17:36:56Z 2014-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3582 https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-014-0102-y 1297-9716 https://doi.org/10.1186/s13567-014-0102-y eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BioMed Central BMC veterinary research 45 : 102. (2014)
spellingShingle Chicken Anemia Virus
Chickens
Animal Diseases
Histocytological Analysis
Análisis Histocitológico
Histopathology
Infection
Virus Anemia Pollo
Pollo
Enfermedades de los Animales
Histopatología
Infección
Subclinical Infection
Double Positive Cell
Negative Control Group
Argentina
Rimondi, Agustina
Pinto, Silvina
Olivera, Valeria Soledad
Dibarbora, Marina
Perez Filgueira, Daniel Mariano
Craig, María Isabel
Pereda, Ariel Julian
Comparative histopathological and immunological study of two field strains of chicken anemia virus
title Comparative histopathological and immunological study of two field strains of chicken anemia virus
title_full Comparative histopathological and immunological study of two field strains of chicken anemia virus
title_fullStr Comparative histopathological and immunological study of two field strains of chicken anemia virus
title_full_unstemmed Comparative histopathological and immunological study of two field strains of chicken anemia virus
title_short Comparative histopathological and immunological study of two field strains of chicken anemia virus
title_sort comparative histopathological and immunological study of two field strains of chicken anemia virus
topic Chicken Anemia Virus
Chickens
Animal Diseases
Histocytological Analysis
Análisis Histocitológico
Histopathology
Infection
Virus Anemia Pollo
Pollo
Enfermedades de los Animales
Histopatología
Infección
Subclinical Infection
Double Positive Cell
Negative Control Group
Argentina
url http://hdl.handle.net/20.500.12123/3582
https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-014-0102-y
https://doi.org/10.1186/s13567-014-0102-y
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