Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine
In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors w...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2018
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| Materias: | |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0034528813001161 http://hdl.handle.net/20.500.12123/3579 http://dx.doi.org/10.1016/j.rvsc.2013.03.016 |
| _version_ | 1855483264837353472 |
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| author | Gonzalez, Diego Rimondi, Agustina Perez Aguirreburialde, Maria Sol Mozgovoj, Marina Valeria Bellido, Demian Wigdorovitz, Andres Dus Santos, Maria Jose |
| author_browse | Bellido, Demian Dus Santos, Maria Jose Gonzalez, Diego Mozgovoj, Marina Valeria Perez Aguirreburialde, Maria Sol Rimondi, Agustina Wigdorovitz, Andres |
| author_facet | Gonzalez, Diego Rimondi, Agustina Perez Aguirreburialde, Maria Sol Mozgovoj, Marina Valeria Bellido, Demian Wigdorovitz, Andres Dus Santos, Maria Jose |
| author_sort | Gonzalez, Diego |
| collection | INTA Digital |
| description | In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors were involved in the protection observed. In this work, groups of cows (n = 4) were immunized in the last third of gestation with a bovine rotavirus (BRV) experimental vaccine and with a VP6 subunit vaccine. At birth, colostrums from vaccinated and non-vaccinated cows were processed and viable colostral mononuclear cells were obtained. With the purpose of determining the cytokine patterns generated by cells from immune secretions (colostrums and milk), a relative quantification by real time PCR was standardized. Quantitative real time PCR (qPCR) was used to determine transcript levels of IL-4, IL-6, IL-10, IL-12, IFN-γ and IFN-α from these cells. Colostral and milk mononuclear cells expressed a different cytokine transcript expression pattern regarding the vaccine used. These results demonstrated that the colostral cellular population was active and could exert its action influencing the final immune response. |
| format | Artículo |
| id | INTA3579 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA35792019-01-23T13:41:13Z Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine Gonzalez, Diego Rimondi, Agustina Perez Aguirreburialde, Maria Sol Mozgovoj, Marina Valeria Bellido, Demian Wigdorovitz, Andres Dus Santos, Maria Jose Gene Expression Colostrum PCR Cytokines Cow Milk Synthetic Vaccines Expresión Génica Calostro Citoquinas Leche de Vaca Vacuna Sintética VP6 Subunit Vaccine Polymerase Chain Reaction In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors were involved in the protection observed. In this work, groups of cows (n = 4) were immunized in the last third of gestation with a bovine rotavirus (BRV) experimental vaccine and with a VP6 subunit vaccine. At birth, colostrums from vaccinated and non-vaccinated cows were processed and viable colostral mononuclear cells were obtained. With the purpose of determining the cytokine patterns generated by cells from immune secretions (colostrums and milk), a relative quantification by real time PCR was standardized. Quantitative real time PCR (qPCR) was used to determine transcript levels of IL-4, IL-6, IL-10, IL-12, IFN-γ and IFN-α from these cells. Colostral and milk mononuclear cells expressed a different cytokine transcript expression pattern regarding the vaccine used. These results demonstrated that the colostral cellular population was active and could exert its action influencing the final immune response. Instituto de Virología Fil: Gonzalez, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Perez Aguirreburualde, Maria Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Bellido, Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina 2018-10-10T18:24:48Z 2018-10-10T18:24:48Z 2013-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0034528813001161 http://hdl.handle.net/20.500.12123/3579 0034-5288 http://dx.doi.org/10.1016/j.rvsc.2013.03.016 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Research in veterinary science 95 (2) : 703-708. (Octubre 2013) |
| spellingShingle | Gene Expression Colostrum PCR Cytokines Cow Milk Synthetic Vaccines Expresión Génica Calostro Citoquinas Leche de Vaca Vacuna Sintética VP6 Subunit Vaccine Polymerase Chain Reaction Gonzalez, Diego Rimondi, Agustina Perez Aguirreburialde, Maria Sol Mozgovoj, Marina Valeria Bellido, Demian Wigdorovitz, Andres Dus Santos, Maria Jose Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine |
| title | Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine |
| title_full | Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine |
| title_fullStr | Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine |
| title_full_unstemmed | Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine |
| title_short | Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine |
| title_sort | quantitation of cytokine gene expression by real time pcr in bovine milk and colostrum cells from cows immunized with abovine rotavirus vp6 experimental vaccine |
| topic | Gene Expression Colostrum PCR Cytokines Cow Milk Synthetic Vaccines Expresión Génica Calostro Citoquinas Leche de Vaca Vacuna Sintética VP6 Subunit Vaccine Polymerase Chain Reaction |
| url | https://www.sciencedirect.com/science/article/pii/S0034528813001161 http://hdl.handle.net/20.500.12123/3579 http://dx.doi.org/10.1016/j.rvsc.2013.03.016 |
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