Mapping QTL for resistance to new virulent races of wheat stripe rust from two Argentinean wheat cultivars
During the last two decades, new virulent and aggressive races of Puccinia striiformis Westend. f. sp. tritici (Pst) have spread worldwide, causing devastating epidemics and prompting the search for new sources of resistance in wheat (Triticum aestivum L.). Between 2012 and 2017, we mapped four stri...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Crop Science Society of America
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/3564 https://dl.sciencesocieties.org/publications/cs/articles/0/0/cropsci2018.04.0286 |
| _version_ | 1855035164570157056 |
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| author | Cobo, Nicolás Pfluger, Laura Alicia Chen, Xianming Dubcovsky, Jorge |
| author_browse | Chen, Xianming Cobo, Nicolás Dubcovsky, Jorge Pfluger, Laura Alicia |
| author_facet | Cobo, Nicolás Pfluger, Laura Alicia Chen, Xianming Dubcovsky, Jorge |
| author_sort | Cobo, Nicolás |
| collection | INTA Digital |
| description | During the last two decades, new virulent and aggressive races of Puccinia striiformis Westend. f. sp. tritici (Pst) have spread worldwide, causing devastating epidemics and prompting the search for new sources of resistance in wheat (Triticum aestivum L.). Between 2012 and 2017, we mapped four stripe rust resistance quantitative trait loci (QTL) effective against the Pst races present in California, USA, using recombinant inbred lines (RILs) developed from the cross between the Argentinean cultivars ‘Klein Proteo’ and ‘Klein Chajá’. The RIL population showed transgressive segregation in all six growing seasons relative to the parental lines, which showed moderate levels of Pst resistance. Analyses by year detected QTL conferring adult plant resistance on chromosomes 1BL, 2BS, 3D centromeric (from Klein Chajá), and 4DL (from Klein Proteo). QYr.ucw-1BL, mapped in the Yr29 resistance gene region, was significant in all seasons (P < 0.01) and explained on average 31.0 to 32.8% of the observed variation. QYr.ucw-2BS showed a stronger effect than QYr.ucw-1BL in 2013 but was ineffective in 2014 and 2016. This QTL also conferred seedling resistance, suggesting that it is an all-stage resistance gene. Centromeric QYr.ucw-3D and QYr.ucw-4DL showed smaller effects than the previous QTL and were significant only in some of the experiments. No significant interactions were detected among QTL, indicating the absence of digenic epistatic effects. The molecular markers identified in this study can be used to combine these genes and accelerate their deployment in wheat breeding programs. |
| format | info:ar-repo/semantics/artículo |
| id | INTA3564 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Crop Science Society of America |
| publisherStr | Crop Science Society of America |
| record_format | dspace |
| spelling | INTA35642018-10-08T14:10:08Z Mapping QTL for resistance to new virulent races of wheat stripe rust from two Argentinean wheat cultivars Cobo, Nicolás Pfluger, Laura Alicia Chen, Xianming Dubcovsky, Jorge Wheat Varieties Plant Diseases Rusts Disease Resistance Quantitative Trait Loci Genetic Markers Trigo Variedades Enfermedades de las Plantas Roya Resistencia a la Enfermedad Loci de Rasgos Cuantitativos Triticum Aestivum Puccinia Striiformis Marcadores Genéticos Argentina QTL During the last two decades, new virulent and aggressive races of Puccinia striiformis Westend. f. sp. tritici (Pst) have spread worldwide, causing devastating epidemics and prompting the search for new sources of resistance in wheat (Triticum aestivum L.). Between 2012 and 2017, we mapped four stripe rust resistance quantitative trait loci (QTL) effective against the Pst races present in California, USA, using recombinant inbred lines (RILs) developed from the cross between the Argentinean cultivars ‘Klein Proteo’ and ‘Klein Chajá’. The RIL population showed transgressive segregation in all six growing seasons relative to the parental lines, which showed moderate levels of Pst resistance. Analyses by year detected QTL conferring adult plant resistance on chromosomes 1BL, 2BS, 3D centromeric (from Klein Chajá), and 4DL (from Klein Proteo). QYr.ucw-1BL, mapped in the Yr29 resistance gene region, was significant in all seasons (P < 0.01) and explained on average 31.0 to 32.8% of the observed variation. QYr.ucw-2BS showed a stronger effect than QYr.ucw-1BL in 2013 but was ineffective in 2014 and 2016. This QTL also conferred seedling resistance, suggesting that it is an all-stage resistance gene. Centromeric QYr.ucw-3D and QYr.ucw-4DL showed smaller effects than the previous QTL and were significant only in some of the experiments. No significant interactions were detected among QTL, indicating the absence of digenic epistatic effects. The molecular markers identified in this study can be used to combine these genes and accelerate their deployment in wheat breeding programs. Instituto de Recursos Biológicos Fil: Cobo, Nicolás. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos Fil: Pfluger, Laura Alicia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Chen, Xianming. Washington State Univ. Wheat Health, Genetics and Quality Research Unit. Department of Plant Pathology; Estados Unidos Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos 2018-10-08T13:59:59Z 2018-10-08T13:59:59Z 2018 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3564 https://dl.sciencesocieties.org/publications/cs/articles/0/0/cropsci2018.04.0286 0011-183X 1435-0653 (Online) 10.2135/cropsci2018.04.0286 eng info:eu-repo/semantics/restrictedAccess application/pdf Crop Science Society of America Crop science 58 : 1-14. (November–december 2018) |
| spellingShingle | Wheat Varieties Plant Diseases Rusts Disease Resistance Quantitative Trait Loci Genetic Markers Trigo Variedades Enfermedades de las Plantas Roya Resistencia a la Enfermedad Loci de Rasgos Cuantitativos Triticum Aestivum Puccinia Striiformis Marcadores Genéticos Argentina QTL Cobo, Nicolás Pfluger, Laura Alicia Chen, Xianming Dubcovsky, Jorge Mapping QTL for resistance to new virulent races of wheat stripe rust from two Argentinean wheat cultivars |
| title | Mapping QTL for resistance to new virulent races of wheat stripe rust from two Argentinean wheat cultivars |
| title_full | Mapping QTL for resistance to new virulent races of wheat stripe rust from two Argentinean wheat cultivars |
| title_fullStr | Mapping QTL for resistance to new virulent races of wheat stripe rust from two Argentinean wheat cultivars |
| title_full_unstemmed | Mapping QTL for resistance to new virulent races of wheat stripe rust from two Argentinean wheat cultivars |
| title_short | Mapping QTL for resistance to new virulent races of wheat stripe rust from two Argentinean wheat cultivars |
| title_sort | mapping qtl for resistance to new virulent races of wheat stripe rust from two argentinean wheat cultivars |
| topic | Wheat Varieties Plant Diseases Rusts Disease Resistance Quantitative Trait Loci Genetic Markers Trigo Variedades Enfermedades de las Plantas Roya Resistencia a la Enfermedad Loci de Rasgos Cuantitativos Triticum Aestivum Puccinia Striiformis Marcadores Genéticos Argentina QTL |
| url | http://hdl.handle.net/20.500.12123/3564 https://dl.sciencesocieties.org/publications/cs/articles/0/0/cropsci2018.04.0286 |
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